Summary
A somatic hybridisation programme was undertaken to evaluate the expression of reducing sugar accumulation in potato. Interspecific hybrids created between the Solanum tuberosum cultivar Record and the diploid species Solanum phureja were evaluated at the morphological and molecular levels. These analyses indicated that the protoplast regenerants were partial (asymmetric) hybrids which had undergone elimination of S. phureja chromosomes. Tubers of the parents exhibited significant differences for reducing sugar accumulation during cold storage with S. phureja having lower levels of glucose and fructose than Record. The somatic hybrids resembled the S. phureja parent in terms of reducing sugar accumulation demonstrating that low reducing sugar accumulation is dominant to high reducing sugar accumulation in these particular genotypes. These results are discussed in relation to the exploitation of asymmetric hybridisation for the production of potato genotypes for the potato processing industry.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- BSA:
-
(Bovine serum albumin)
- GA3 :
-
(Gibberellic acid)
- MS:
-
(Murashige and Skoog)
- NAA:
-
(α-Naphthaleneacetic acid)
- RAPD:
-
(Randomly Amplified Polymorphic DNA)
References
Austin S, Baer M, Ehlenfeldt M, Kazmierczak, Helgeson PJ (1985a) Theor Appl Genet 71: 172–175.
Austin S, Baer M, Helgeson JP (1985b) Plant Sci Lett 39: 75–81.
Baird E, Cooper-Bland S, Waugh R, De Maine M, Powell W (1992) Mol Gen Genet 233: 469–475.
Barsby TL, Shepard JF, Kemble RJ, Wong R (1984) Plant Cell Rep 3: 165–169.
Brown J, Mackay GR, Bain H, Griffiths DW, Allison MJ (1990) Pot. Res. 33: 219–227.
Butenko RG, Kuchko AA (1980) In: Ferenczy L, Farkas GL (eds) Advances in Protoplast Research. Oxford, Pergamon Press, pp. 293–300.
Chaput MH, Sihachakr D, Ducreux G, Marie D, Barghi N (1990) Plant Cell Rep 98: 411–415.
Colon LT, Sijpkes, Hartmans KJ (1989) In: Louwes KM, Toussaint HAJM, Dellaent LMW (eds) Parental line breeding and selection in potato breeding. Pudoc, Wageningen, pp. 76–80
Fish N, Karp A (1986) Theor Appl Genet 72: 405–412.
Fish N, Karp A, Jones MGK (1987) In Vitro 23: 575–580.
Fish N, Karp A, Jones MGK (1988) Theor Appl Genet 76: 260–266.
Foulgar D, Jones MGK (1986) Plant Cell Rep 5: 72–76.
Glimelius K, Fahlesson J, Landgren M, Sjödin, Sundberg E (1991) Trends in Biotechnology 9: 24–30.
Machray G, Hedley P, Waugh R, Burch L, Cuthbert E, Davies HV (1991) SCRI Annual Report, 1991: 40–41.
Mackay GR, Brown J, Torrance CJW (1990) Pot Res 33: 211–218.
Mackay GR, Dale MFB (1990) ‘Quality in Potatoes’. SCRI Annual Report 1990.
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497.
Nelson S, Creissen GP, Bright SWJ (1983) Plant Sci Lett 30: 355–362.
Pijnacker LP, Ferwerda MA, Puite KJ, Roest S (1987) Theor Appl Genet 73: 878–882.
Pijnacker LP, Ferwerda MA, Puite KJ, Schaart JG (1989) Plant Cell Rep 8: 82–85.
Puite KJ, Roest S, Pijnacker LP (1986) Plant Cell Rep 5: 262–265.
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Proc Natl Acad Sci USA 81: 8014–8018.
Shepard JF, Totten RE (1977) Plant Physiol 60: 313–316.
Sidorov VA, Subko MK, Kuchko AA, Komarnitsky IK, Gleba YY (1987) Theor Appl Genet 74: 364–368.
Viola R, Davies HV (1992) Pot Res 35: 55–58.
Waugh R, Powell W (1992) Trends in Biotechnology 10: 186–191.
Author information
Authors and Affiliations
Additional information
Communicated by M. R. Davey
Rights and permissions
About this article
Cite this article
Craig, A.L., Morrison, I., Baird, E. et al. Expression of reducing sugar accumulation in interspecific somatic hybrids of potato. Plant Cell Reports 13, 401–405 (1994). https://doi.org/10.1007/BF00234147
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00234147