Abstract
PCR with oligonucleotide primers that corresponded to two highly homologous regions, in terms of amino acid sequence, of plant peroxidases was used to amplify a specific DNA fragment from a mixture of rice (Oryza sativa L.) cDNAs. We then screened a cDNA library prepared from mRNAs of rice shoots utilizing the product of PCR as probe. Two cDNA clones, prxRPA and prxRPN, were isolated. They encode distinct isozymes of peroxidase. Sequence analysis indicated that the clones encode mature proteins of approximately 32 kDa, both of which possess a putative signal peptide. Comparison of the amino acid sequences of the two rice peroxidases showed that they are about 70% similar to each other but are only 40% to 50% similar to other plant peroxidases. RNA blot hybridization revealed that mRNAs that corresponded to prxRPA and prxRPN cDNAs accumulate at high levels in roots but only at low levels in stems and leaves. In various tissues of rice plants, levels of both mRNAs were stimulated by wounding and by ethephon. These results indicate that at least two isozymes of peroxidase are expressed not only in shoots but also in roots of rice plants, and that the expression of these genes is influenced by ethylene which is the simplest plant hormone.
Similar content being viewed by others
References
Abeles FB, Dunn LJ, Morgens P, Callahan A, Dinterman RE, Schmidt J (1988) Plant Physiol. 87: 609–615
Acosta M, Arnao MB, Casas JL, De Rio JA, Vioque B, Fernandez-Maculet JC (1992) Biochemical, Molecular, and Physiological Aspects of Plant Peroxidases (Lobarzewski J, Greppin H, Penel C, Caspar TH eds.), pp. 121–125, Imprimerie Nationale, Geneva
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1987) Current Protocols in Molecular Biology, Wiley, New York
Bowles DJ (1990) Annu. Rev. Biochem. 59: 873–907
Buffard D, Breda C, van Huystee RB, Asemota O, Pierre M, Dang Ha DB, Esnault R (1990) Proc. Natl. Acad. Sci. USA 87: 8874–8878
Espelie KE, Franceschi VR, Kolattukudy PE (1986) Plant Physiol. 81: 487–492
Fujiyama K, Takemura H, Shibayama S, Kobayashi K, Choi J-K, Shinmyo A, Takano M, Yamada Y, Okada H (1988) Eur. J. Biochem. 173: 681–687
Fujiyama K, Takemura H, Shinmyo A, Okada H, Takano M (1990) Gene 89: 163–169
Gubler U, Hoffman BJ (1983) Gene 25: 263–269
Hammerschmidt R, Nuckles E, Kuc J (1982) Physiol. Plant Pathol. 20: 73–82
Hertig C, Rebmann G, Bull J, Mauch F, Dudler R (1991) Plant Mol. Biol. 16: 171–174
Hinnman RL, Lang J (1965) Biochemistry 4:144–154
Intapruk C, Higashimura N, Yamamoto K, Okada N, Shinmyo A, Takano M (1990) Gene 98: 237–241
Ishino Y, Uemori T, Fujita K, Asada K, Kato I, J. Biol. Chem., submitted
Ito H, Hiraoka N, Ohbayashi A, Ohashi Y (1991) Agric. Biol. Chem. 55: 2445–2454
Kay LE, Basile DV (1987) Plant Physiol. 84: 99–105
Lagrimini LM, Rothstein S (1987) Plant Physiol. 84: 438–442
Lagrimini LM, Burkhart W, Moyer M, Rothstein S (1987) Proc. Natl. Acad. Sci. USA 84: 7542–7546
Mazza G, Welinder KG (1980) Eur. J. Biochem. 108: 481–489
Mittal R, Dubey S (1991) Plant Physiol. Biochem. 29: 31–40
Mohan R, Bajar AM, Kolattukudy PE (1993) Plant Mol. Biol. 21: 341–354
Morgens PH, Callahan AM, Dunn LJ, Abeles FB (1990) Plant Mol. Biol. 14: 715–725
Muller A, Grafe R (1978) Mol. Gen. Genet. 161: 67–76
Murray EE, Lotzer J, Eberle M (1989) Nucl. Acids Res. 17: 477–498
Murray MG, Thompson WF (1980) Nucl. Acids Res. 8: 4321–4325
Negrel J, Lherminier J, (1987) Planta 172: 494–501
Rasmussen SK, Welinder KG, Hejgaard J (1991) Plant Mol. Biol. 16: 317–327
Rebman G, Hertig C, Bull J, Mauch F, Dudler R (1991) Plant Mol. Biol. 16: 329–331
Roberts E, Kutchan T, Kolattukudy PE (1988) Plant Mol. Biol. 11: 15–26
Roberts E, Kolattukudy PE (1989) Mol. Gen. Genet. 217: 223–232
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) Proc. Natl. Acad. Sci. USA 74: 5463–5467
Sheehy RE, Honma M, Yamada M, Sasaki T, Martineau B, Hiatt WR (1991) J. Bacteriol. 173: 5260–5265
Sheen SJ, Diachun S (1978) Acta Phytopathologica Academiae Scientiarum Hungaricae 13: 21–28
Sheets MD, Ogg SC, Wickens MP (1990) Nucl. Acids Res. 18: 5799–5805
Shinmyo A (1988) Cell Technology 7: 588–590 (in Japanese)
Svalheim O, Robertsen B (1990) Plant Physiol. 78: 261–267
Theilade B, Rasmussen SK (1992) Gene 118: 261–266
van Huystee RB (1987) Annal. Rev. Plant physiol. 38: 205–219
Welinder KG (1979) Eur. J. Biochem. 96: 483–502
Yang SF, Hoffman NE (1984) Ann. Rev. Plant Physiol. 35: 155–189
Zheng X, van Huystee RB (1992) Plant Science 81: 47–56
Author information
Authors and Affiliations
Additional information
Communicated by A. Komamine
Rights and permissions
About this article
Cite this article
Ito, H., Kimizuka, F., Ohbayashi, A. et al. Molecular cloning and characterization of two complementary DNAs encoding putative peroxidases from rice (Oryza sativa L.) shoots. Plant Cell Reports 13, 361–366 (1994). https://doi.org/10.1007/BF00234138
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00234138