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Regeneration of simon poplar (Populus simonii) from protoplast culture

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Abstract

Simon poplar (Populus simonii) protoplasts were isolated from suspension cells, with protoplast yield of 3.8×107 g−1 F. W. They were cultured in a K8P liquid medium containing 13.57μM 2,4-D, 1.07μM NAA and 0.93 μM KT. Protoplast culture was influenced by the plating density, osmotic pressure, and the sources and amounts of nitrogen and carbon in the culture medium. Multiple shoots were produced from protoplast-derived callus after culture on MS medium containing 4.44 μM BA, 2.32μM KT, 2.28 μM ZT, and 0.54μM NAA. Shoots 2–3 cm in height were isolated from the calli and rooted on 1/2 MS medium. After transplantation into pots, the regenerated plants grew vigorously in greenhouse.

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Abbreviations

BA:

N6-benzyladenine

NAA:

1-naphthalene acetic acid

2,4-D:

2,4-dichlorophenoxy acetic acid

KT:

Kinetin

ZT:

Zeatin

2ip:

2-isopentenyl-adenine

FDA:

fluorescein diacetate

MES:

2-(N-morpholino) ethane sulfonic acid

MS:

Murashige and Skoog basal medium (1962)

K8P:

Kao basal medium (1977)

CPW:

Cell and Protoplast Wash solution (Power and Davey 1980)

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Authors and Affiliations

  1. Forest tree Genetics and Breeding Laboratory, Nanjing Forestry University, Longpan Road, 210037, Nanjing, China

    Y. Wang, M. R. Huang, D. M. Chen & N. Xu

  2. National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Academia Sinica, 200032, Shanghai, China

    Z. M. Wei & Z. H. Xu

  3. Institute of Genetics, Academia Sinica, 100101, Beijing, China

    Y. R. Sun & L. M. Zhang

Authors
  1. Y. Wang
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  2. M. R. Huang
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  3. Z. M. Wei
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  4. Y. R. Sun
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  5. D. M. Chen
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  6. Z. H. Xu
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  7. L. M. Zhang
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  8. N. Xu
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Communicated by G.C. Phillips

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Wang, Y., Huang, M.R., Wei, Z.M. et al. Regeneration of simon poplar (Populus simonii) from protoplast culture. Plant Cell Reports 14, 442–445 (1995). https://doi.org/10.1007/BF00234051

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  • DOI: https://doi.org/10.1007/BF00234051

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