Summary
The cell membrane of smooth muscle cells from four large arteries of rabbits was investigated by freeze-fracture. On the surface of the smooth muscle smooth zones could be distinguished arranged in an alternate manner with zones characterised by numerous openings of surface vesicles. Vesiculate zones formed bands of variable width, generally parallel to the long axis of the cell, or irregular patches of different size. The density of vesicles within bands and patches varied from a very loose packing to a dense, quasi-hexagonal array. Comparative morphometric studies of the cell membrane replicas showed differences in the ratio vesiculate area: smooth area and also different surface vesicle frequencies expressed either as mean values or as values for vesiculate zones, in the arteries investigated. Communicating junctions (nexuses, gap junctions) characterized by a random arrangement of particles on the P-face and a more ordered, rhomboidal array of pits on the E-face were the only type of intercellular junctions found between arterial smooth muscle cells.
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References
Branton D, Bullivant S, Gilula NB, Karnovsky MJ, Moore H, Mühlethaler K, Northcote DH, Packer L, Satir B, Satir P, Speth V, Staehelin LA, Steere LR, Weinstein RS (1975) Freeze-etching nomenclature. Science (Wash) 190:54–56
Büssow H, Wulfhekel U (1972) Die Feinstruktur der glatten Muskelzellen in den großen muskulären Arterien der Vögel. Z Zellforsch 125:339–352
Caesar R, Edwards GA, Ruska H (1957) Architecture and nerve supply of mammalian smooth muscle tissue. J Biophys Biochem Cytol 3:867–878
Cliff WJ (1967) The aortic tunica media in growing rats studied with the electron microscope. Lab Invest 17:599–615
Cobb JLS, Bennett T (1969) A study of nexuses in visceral smooth muscle. J Cell Biol 41:287–297
Devine CE, Simpson FO (1968) The morphological basis for the sympathetic control of blood vessels. N Z Med J 67:326–334
Devine CE, Simpson FO, Bertaud WS (1971) Surface features of smooth muscle cells from the mesenteric artery and vas deferens. J Cell Sci 9:427–443
Devine CE, Somlyo AV, Somlyo AP (1972) Sarcoplasmic reticulum and excitation-contraction coupling in mammalian smooth muscle. J Cell Biol 52:690–718
Forbes MS, Rennels ML, Nelson E (1979) Caveolar systems and sarcoplasmic reticulum in coronary smooth muscle cells of the mouse. J Ultrastruct Res 67:325–339
Gabella G (1971) Caveolae intracellulares and sarcoplasmic reticulum in smooth muscle. J Cell Sci 8:601–609
Gorgas K, Böck P (1976) Studies on intra-arterial cushions. II. Distribution of horseradish peroxidase in cushions at the origins of intercostal arteries in mice. Anat Embryol (Berlin) 149:315–321
Henderson RM (1975) Types of cell contacts in arterial smooth muscle. Experientia (Basel) 31:103–105
Iwayama T (1971) Nexuses between areas of the surface membrane of the same arterial smooth muscle. J Cell Biol 49:521–525
Lane BP (1965) Alterations in the cytologic detail of intestinal smooth muscle cell in various stages of contraction. J Cell Biol 27:199–213
Muggli R, Baumgartner HR (1972) Patterns of membrane invaginations at the surface of smooth muscle cells of rabbit arteries. Experientia (Basel) 28:1212–1214
Nagasawa J, Suzuki T (1967) Electron microscopic study on the cellular interrelationships in the smooth muscle. Tohouku J Exp Med 91:299–313
Orci L, Perrelet A (1973) Membrane-associated particles: Increase at sites of pinocytosis demonstrated by freeze-etching. Science (Wash) 181:868–869
Pease DC, Molinari S (1960) Electron microscopy of muscular arteries: pial vessels of the cat and monkey. J Ultrastruct Res 3:447–468
Prosser CL, Burnstock G, Kahn J (1960) Conduction in smooth muscle: comparative structural properties. Am J Physiol 199:545–552
Rhodin JAG (1967) The ultrastructure of mammalian arterioles and precapillary sphincters. J Ultrastruct Res 18:181–223
Satir B, Schooley C, Satir P (1972) Membrane reorganization during secretion in Tetrahymena. Nature (Lond) 235:53–54
Satir B, Schooley C, Satir P (1973) Membrane fusion in a model system. Mucocyst secretion in Tetrahymena. J Cell Biol 56:153–176
Somlyo AP, Somlyo AV (1975) Ultrastructure of smooth muscle. In: Daniel EE, Paton DM (eds) Methods in pharmacology. Plenum Press, New York, pp 3–45
Tani E, Yamagata S, Ito Y (1977) Cell membrane structure of vascular smooth muscle of circle of Willis. Cell Tissue Res 179:131–142
Thyberg J, Hinek A, Nilsson J, Friberg U (1979) Electron microscopic and cytochemical studies of rat aorta intracellular vesicles containing elastin-like and collagen-like material. Histochem J 11:1–18
Uehara Y, Burnstock G (1970) Demonstration of “gap junction” between smooth muscle cells. J Cell Biol 44:215–217
Verity MA, Bevan JA (1968) Fine structural study on the terminal effector plexus, neuromuscular and intermuscular relationships in the pulmonary artery. J Anat (Lond) 103:49–63
Wells GS, Wolowyk MW (1971) Freeze-etch observations on a membrane structure in the smooth muscle of guinea-pig taenia coll. J Physiol (Lond) 218:11p-13p
Wootton GS, Goodford PJ (1975) An association between mitochondria and vesicles in smooth muscle. Cell Tiss Res 161:119–132
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Litwin, J.A. Cell membrane features of rabbit arterial smooth muscle. Cell Tissue Res. 212, 341–350 (1980). https://doi.org/10.1007/BF00233966
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DOI: https://doi.org/10.1007/BF00233966