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An extensive hairy root production precedes shoot regeneration in protoplast-derived calli of cauliflower (Brassica oleracea var. botrytis)

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Summary

Protoplasts isolated from mesophyll tissues of cauliflower (Brassica oleraceavar. botrytis) were induced to divide in culture, with 2% of them producing calli. Upon transfer to a regeneration medium containing a low auxin/cytokinin balance (0.02mg/l 1-naphthaleneacetic and 2mg/l 6-benzylaminopurine), they displayed an extensive production of hairy roots before the regeneration of shoots. Negative effects of root differentiation on the subsequent caulogenesis by such calli were not observed, since 97% of the calli regenerated hairy roots and 93% gave shoots.

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Abbreviations

NAA:

1-naphthaleneacetic acid

BAP:

6-benzylaminopurine

2,4 D:

2,4-dichlorophenoxyacetic acid

GA3:

gibberellic acid

MES:

2-N-morpholinoethane sulfonate

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Authors and Affiliations

  1. Lab. Amel. Pl. et Biotechnologies, ENITHP, 2 rue le Nôtre, 49045, Angers, France

    N. Delpierre & J. Boccon-Gibod

Authors
  1. N. Delpierre
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  2. J. Boccon-Gibod
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Communicated by A. M. Boudet

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Delpierre, N., Boccon-Gibod, J. An extensive hairy root production precedes shoot regeneration in protoplast-derived calli of cauliflower (Brassica oleracea var. botrytis). Plant Cell Reports 11, 351–354 (1992). https://doi.org/10.1007/BF00233364

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  • DOI: https://doi.org/10.1007/BF00233364

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