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Transient gene expression in electroporated banana (Musa spp., cv. ‘Bluggoe’, ABB group) protoplasts isolated from regenerable embryogenetic cell suspensions

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Summary

Electroporation conditions were established for transient expression of introduced DNA in banana (Musa spp., cv. ‘Bluggoe’) protoplasts isolated from regenerable embryogenic cell suspensions. The following parameters were found to be highly influential: electroporation buffer, polyethylene glycol treatment and its duration before electroporation, use of a heat shock, and chimaeric gene constructs. The maximum frequency of DNA introduction as detected by an in situ assay for transient expression of the uidA gene, amounted to 1.8% of total protoplasts. Since plants have recently been regenerated from banana protoplasts at a high frequency, the present results may contribute to the production of transgenic banana.

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Abbreviations

AMV:

alfalfa mosaic virus

CaMV:

cauliflower mosaic virus

2,4-D:

2,4-dichlorophenoxyacetic acid

EGTA:

ethylene glycol-O-O'-bis(2-aminoethyl)-N,N,N',N'-tetraacetic acid

GUS:

βglucuronidase

HEPES:

4-(2-nydroxyethyl)piperazine-1-etnanesulfonic acid

MES:

2-morpholinoethanesulfonic acid

MS:

Murashige-Skoog

NOS:

nopaline synthase

NFTII:

neomycin phosphotransferase

PEG:

polyethylene glycol

TGE:

transient GUS expression

X-Gluc:

5-bromo-4-chloro-3-indolyl β-D-glucuronic acid

References

  • Bates GW, Piastuch W, Riggs CD, Rabussay D (1988) Plant Cell Tiss Org Cult 12:213–218

    Google Scholar 

  • Bekkaoui F, Datla RSS, Pilon M, Tautoms TE, Crosby WL, Dunstan DI (1990) Theor Appl Genet 79:353–359

    Google Scholar 

  • Boston RS, Becwar MR, Ryan RD, Goldsbrough PB, Larkins BA, Hodges TK (1987) Plant Physiol 83:742–746

    Google Scholar 

  • Bower R, Birch RG (1990) Plant Cell Rep 9:386–389

    Google Scholar 

  • Chupeau M-C, Bellini C, Guerche P, Maisonneuve B, Vastra G, Chupeau Y (1989) Bio/Technology 7:503–508

    Google Scholar 

  • Dhed'a D (1992) Ph. D. thesis. Faculty of Agricultural Sciences, Catholic University of Leuven, Belgium, 167 pp.

    Google Scholar 

  • Dhed'a D, Dumortier F, Panis B, Vuylsteke D, De Langhe E (1991) Fruits 46:125–135

    Google Scholar 

  • Dhir SK, Dhir S, Hepburn A, Widholm JM (1991) Plant Cell Rep 10:106–110

    Google Scholar 

  • Diaz I, Carbonero P (1992) Plant Cell Rep 10:595–598

    Google Scholar 

  • Escalant JV, Teisson C (1989) Plant Cell Rep 7:665–668

    Google Scholar 

  • FAO (1992) Production yearbook 1991. Food and Agriculture Organisation of the United Nations. Rome, Italy, 265 pp.

    Google Scholar 

  • Fromm M, Taylor LP, Walbot V (1985) Proc Natl Acad Sci USA 82:5824–5828

    Google Scholar 

  • Fromm ME, Taylor LP, Walbot V (1986) Nature 319:791–793

    Google Scholar 

  • Hauptmann RM, Ozias-Akins P, Vasil V, Tabaeizadeh Z, Rogers SG, Horsch RB, Vasil IK, Fraley RT (1987) Plant Cell Rep 6:265–270

    Google Scholar 

  • Huang Y-W, Dennis ES (1989) Plant Cell Tiss Org Cult 18:281–296

    Google Scholar 

  • Jefferson RA (1987) Plant Mol Biol Rep 5:387–405

    CAS  Google Scholar 

  • Jefferson RA, Burgess SM, Hirsh D (1986) Proc Natl Acad Sci USA 83:8447–8451

    CAS  PubMed  Google Scholar 

  • Kao KN, Michayluk MR (1974) Planta 115:355–367

    Google Scholar 

  • Lindsey K, Jones MGK (1989) Plant Cell Rep 8:71–74

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol Plant 15:473–497

    CAS  Google Scholar 

  • Negrutiu I, Dewulf J, Pietrzak M, Botterman J, Rietveld E, Wurzer-Figurelli EM, Ye D, Jacobs M (1990) Physiol Plant 79:197–205

    Google Scholar 

  • Oard JH, Paige D, Dvorak J (1989) Plant Cell Rep 8:156–160

    Google Scholar 

  • Panis B, Dhed'a D, Swennen R (1991) In: Adams RP, Adams JE (eds) Conservation of plant genes: DNA banking and in vitro biotechnology. Academic Press, New York, pp 183–195

    Google Scholar 

  • Panis B, Van Wauwe A, Swennen R (1993) Plant Cell Rep 12:403–407

    Google Scholar 

  • Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) EMBO J 3:2717–2722

    Google Scholar 

  • Planckaert F, Walbot V (1989) Plant Cell Rep 8:144–147

    Google Scholar 

  • Potrykus I, Saul MW, Petruska J, Paszkowski J, Shillito RD (1985) Mol Gen Genet 199:183–188

    Google Scholar 

  • Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ (1988) Science 240:204–207

    Google Scholar 

  • Riggs CD, Bates GW (1986) Proc Natl Acad Sci USA 83:5602–5606

    Google Scholar 

  • Rouan D, Montoné M-H, Alibert G, Teissié J (1991) Plant Cell Rep 10:139–143

    Google Scholar 

  • Sagi L, Remy S, Panis B, Swennen R, Volckaert G (1992) Banana Newsletter 15:42

    Google Scholar 

  • Séguin A, Lalonde M (1988) Plant Cell Rep 7:367–370

    Google Scholar 

  • Shillito RD, Saul MW, Paszkowski J, Muller M, Potrykus I (1985) Bio/Technology 3:1099–1103

    Google Scholar 

  • Shimamoto K, Terada R, Izawa T, Fujimoto H (1989) Nature 338:274–276

    Google Scholar 

  • Tada Y, Sakamoto M, Fujimura T (1990) Theor Appl Genet 80:475–480

    Google Scholar 

  • Tautorus TE, Bekkaoui F, Pilon M, Datla RSS, Crosby WL, Fowke LC, Dunstan DI (1989) Theor Appl Genet 78:531–536

    Google Scholar 

  • Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Bio/Technology 6:1072–1074

    Google Scholar 

  • Tyagi S, Spoerlein B, Tyagi AK, Herrmann RG, Koop HU (1989) Theor Appl Genet 78:287–292

    Google Scholar 

  • van den Hoff MJB, Moorman AFM, Lamers WH (1992) Nucleic Acids Res 20:2902

    Google Scholar 

  • Vuylsteke D, De Langhe E (1985) Tropical Agriculture (Trinidad) 62:323–328

    Google Scholar 

  • Zhang W, Wu R (1988) Theor Appl Genet 76:835–840

    Google Scholar 

Download references

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Author notes

  1. Laszlo Sagi & Serge Remy

    Present address: Laboratory of Tropical Crop Husbandry, Catholic University of Leuven, Kardinaal Mercierlaan 92, B-3001, Leuven, Belgium

Authors and Affiliations

  1. Laboratory of Gene Technology, Catholic University of Leuven, Willem de Croylaan 42, B-3001, Leuven, Belgium

    Laszlo Sagi, Serge Remy & Guido Volckaert

  2. Laboratory of Tropical Crop Husbandry, Catholic University of Leuven, Kardinaal Mercierlaan 92, B-3001, Leuven, Belgium

    Bart Panis & Rony Swennen

Authors
  1. Laszlo Sagi
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  2. Serge Remy
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  3. Bart Panis
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  4. Rony Swennen
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  5. Guido Volckaert
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Additional information

Communicated by H. Lörz

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Sagi, L., Remy, S., Panis, B. et al. Transient gene expression in electroporated banana (Musa spp., cv. ‘Bluggoe’, ABB group) protoplasts isolated from regenerable embryogenetic cell suspensions. Plant Cell Reports 13, 262–266 (1994). https://doi.org/10.1007/BF00233316

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  • DOI: https://doi.org/10.1007/BF00233316

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