Summary
To increase plantlet regeneration frequency, rice callus was dehydrated in a Petri dish with a single layer of filter paper prior to transfer to the regeneration medium. With a 24 h dehydration treatment, the regeneration frequency was increased to 47 %, while the regeneration frequency of the untreated control was less than 5 %. This relatively simple method provides an alternative method for improving the regeneration frequency of rice callus.
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Communicated by M. Tabata
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Tsukahara, M., Hirosawa, T. Simple dehydration treatment promotes plantlet regeneration of rice (Oryza sativa L.) callus. Plant Cell Reports 11, 550–553 (1992). https://doi.org/10.1007/BF00233090
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DOI: https://doi.org/10.1007/BF00233090