Abstract
In vitro plant regeneration via embryogenesis was obtained in suspension cultures of Datura innoxia Mill. Embryogenesis was induced in suspension cultures raised from callus of androgenetic origin, using LS liquid medium supplemented with 0.22 mg/l 2,4-D. The total number of embryos formed was variable over time in culture. Embryos differentiated and matured in the liquid medium itself as also evidenced by histological observation. Embryos germinated to form plantlets on semisolid MS medium without growth substances. The regenerated plants had haploid number of chromosomes.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- Kn:
-
Kinetin
- LS:
-
Linsmaier and Skoog (1965)
- MS:
-
Murashige and Skoog (1962)
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Communicated by G. C. Phillips
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Sharma, V.K., Jethwani, V. & Kothari, S.L. Embryogenesis in suspension cultures of Datura innoxia Mill.. Plant Cell Reports 12, 581–584 (1993). https://doi.org/10.1007/BF00233065
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DOI: https://doi.org/10.1007/BF00233065