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Plant regeneration from protoplasts of peppermint (Mentha piperita L.)

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Summary

Enzymatically isolated leaf-derived protoplasts of peppermint (Mentha piperita L.) were cultured in modified B5 medium containing 1 mg/l NAA, 0.4 mg/l BA, 0.5% sucrose, 0.5 M mannitol and 0.1% Gelrite (first medium). After 30 d culture at 25°C in the dark, protoplasts formed colonies consisting of about 100 cells. Gelrite medium blocks were transferred into liquid medium to promote further growth. Colonies of 0.5 mm transferred to 0.2% Gelrite solidified medium (same components as first medium) formed green calli (1–2 mm) under incubation in the light. Green calli transferred to differentiation medium (B5, 0.1 mg/l NAA, 5 mg/l BA, 2% sucrose, 0.2 M mannitol, 0.2% Gelrite) developed shoot buds after 3–4 weeks. Whole plants were recovered following rooting of shoots in B5 medium without hormones.

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Abbreviations

BA:

6-benzylaminopurine

NAA:

α-naphthaleneacetic acid

KIN:

kinetin

ZEA:

zeatin

CPW:

cell and protoplast wash solution

B5:

Gamborg et al. (1968) mineral elements

MS:

Murashige and Skoog (1962) mineral elements

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Authors and Affiliations

  1. Lotte Central Laboratory Co., Ltd., 3-1-1, Numakage, Urawa, 336, Saitama, Japan

    Hiroshi Sato, Kazuo Hosomi & Yoshio Ito

  2. National Institute of Agrobiological Resources, 2-1-2, Kannondai, Tsukuba, 305, Ibaraki, Japan

    Sueo Enomoto & Seibi Oka

Authors
  1. Hiroshi Sato
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  2. Sueo Enomoto
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  3. Seibi Oka
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  4. Kazuo Hosomi
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  5. Yoshio Ito
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Additional information

Communicated by G. C. Phillips

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Sato, H., Enomoto, S., Oka, S. et al. Plant regeneration from protoplasts of peppermint (Mentha piperita L.). Plant Cell Reports 12, 546–550 (1993). https://doi.org/10.1007/BF00233057

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  • DOI: https://doi.org/10.1007/BF00233057

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