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The influence of the rye genome on expression of heat shock proteins in triticale

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Summary

The heat shock protein profiles from Secale cereale L. cv Imperial, Triticum aestivum L. cv Chinese Spring, S. cereale x T. aestivum amphiploid, and the seven disomic S. cereale addition lines to T. aestivum were used to compare the wheat, rye, and triticale Heat Shock Protein profiles and to study the influence of the rye genome on heat shock protein expression in triticale. Three-day-old seedlings were heat shocked for 2 h at 40 °C in the presence of 35S-methionine, and polypeptides from root tissues were subjected to one- or two-dimensional gel electrophoresis. The wheat and rye heat shock protein profiles each consisted of > 150 heat shock proteins, of which 94 were sufficiently reproducible to construct a standard map. There were 11 unique rye heat shock proteins compared to 22 unique wheat heat shock proteins. The triticale heat shock protein profile resembled the rye parent more than the wheat parent. There were 22 heat shock proteins expressed uniquely by wheat that were not expressed in triticale. Rye chromosomes 1 and 3 exhibited a substantial repressive influence on the expression of 95% of the unique wheat heat shock proteins in triticale, while rye chromosome 4 appeared to have the least repressive influence on expression of the unique wheat heat shock proteins in triticale.

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Authors and Affiliations

  1. J Tuzo Wilson Research Laboratories, Department of Botany, Erindale Campus, University of Toronto, 3359 Mississauga Rd. N., L5L 1C6, Mississauga, Ontario, Canada

    D. J. Somers & W. G. Filion

  2. United States Department of Agriculture, Agricultural Research Service and Department of Agronomy, University of Missouri, 65211, Columbia, MO, USA

    J. P. Gustafson

Authors
  1. D. J. Somers
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  2. J. P. Gustafson
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  3. W. G. Filion
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Communicated by J.W. Snape

Mention of a trade name or proprietary product does not constitute a guarantee, warranty, or recommendation of the product by the United States Department of Agriculture or the University of Missouri and does not imply its approval to the exclusion of other products that may be suitable

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Somers, D.J., Gustafson, J.P. & Filion, W.G. The influence of the rye genome on expression of heat shock proteins in triticale. Theoret. Appl. Genetics 83, 987–993 (1992). https://doi.org/10.1007/BF00232961

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  • DOI: https://doi.org/10.1007/BF00232961

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