Skip to main content
SpringerLink
Log in

Expression of the hygromycin B phosphotransferase gene confers tolerance to the herbicide glyphosate

  • Published:
Plant Cell Reports Aims and scope Submit manuscript

Abstract

Escherichia coli cells and tobacco (cv. Xanthi) plants transformed with the hygromycin B phosphotransferase gene were able to grow in culture medium containing glyphosate at 2.0 mM. The growth of tobacco calli in media containing increasing glyphosate concentrations was measured. The ID50 for glyphosate was 1.70±0.03 mM for hygromycin-B resistant plants, and 0.45±0.02 mM for control plants. Regenerated plants and progeny selected for resistance to hygromycin B were tested for glyphosate tolerance by spraying them with Faena herbicide (formulated glyphosate with surfactant) at a dose equal to 0.24 kg/ha. This was two times the dose required to kill 100 percent of the control plants. Phosphotransferase activity was measured in the extracts of the transformed leaves by the incorporation of 32P from [γ−32P]ATP and it was observed that hygromycin B phosphotransferase was able to recognize the molecule of glyphosate as substrate.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

(Hyg):

Hygromycin

(Km):

Kanamycin

(Glp):

Glyphosate

(Sarc):

Sarcosine

(AMPA):

Aminomethylphosphonic acid

References

  • Comai L, Facciotti D, Hiatt WR, Thompson G, Rose RE, Stalker DM (1985) Nature 317: 741–744.

    Google Scholar 

  • Coruzzi GM (1991) Plant Sci. 74: 145–155.

    Google Scholar 

  • Deblaere R, Bytebier B, De Greve H, Deboeck F, Schell J, Van Montagu M, Leemans J (1985) Nucleic Acids Res. 13: 4777–4788.

    Google Scholar 

  • della-Cioppa G, Bauer SC, Taylor ML, Rochester DE, Klein BK, Shah DM, Fraley RT, Kishore GM (1987) Bio/Tech. 5: 579–584.

    Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) Plant Mol. Biol. Rep. 1: 19–22.

    Google Scholar 

  • Dyer WE, Weller SC, Bressan RA, Herrmann KM (1988) Plant Physiol. 88: 661–666.

    Google Scholar 

  • Feinberg AP, Vogelstein B (1983) Anal Biochem. 132: 6–13.

    Google Scholar 

  • Fillatti J, Kiser J, Rose R, Comai L (1987) Bio/Tech. 5: 726–730.

    Google Scholar 

  • Fitzgibbon JE, Braymer HD (1990) Appl. and Environ. Microbiol. 56: 3382–3388.

    Google Scholar 

  • Forlani G, Nielsen E, Racchi ML (1992) Plant Sci. 85: 9–15.

    Google Scholar 

  • Gabard JM, Charest PJ, Iyer VN, Miki BL (1989) Plant Physiol. 91: 574–580.

    Google Scholar 

  • Goldsbrough PB, Hatch EM, Huang B, Kosinski WG, Dyer WE, Herrmann KM, Weller SC (1990) Plant. Sci. 72: 53–62.

    Google Scholar 

  • Gritz L, Davies J (1983) Gene 25: 179–188.

    Google Scholar 

  • Haas MJ, Dowding JE (1975) 43: 611–628.

  • Hinchee MAW, Connor-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischhoff DA, Re DB, Fraley RT, Horsch RB (1988) Bio/Tech. 6: 917–922.

    Google Scholar 

  • Holländer-Czytko H, Sommer I, Amrhein N (1992) Plant Mol. Biol. 20: 1029–1036.

    Google Scholar 

  • Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT (1985) Science 227: 1229–1231.

    CAS  Google Scholar 

  • Kaster KR, Burgett SG, Nagaraja Rao R, Ingolia TD (1983) Nucleic Acids Res. 11: 6895–6911.

    Google Scholar 

  • Kishore GM, Shah DM (1988) Ann. Rev. Biochem. 57: 227–263.

    Google Scholar 

  • Klee HJ, Muskopf YM, Gasser CS (1987) Mol. Gen Genet. 210: 437–442.

    Google Scholar 

  • Moorman TB, Becerril JM, Lydon J, Duke SO (1992) J. Agri. and Food Chem. 40: 289–293.

    Google Scholar 

  • Mousdale DM, Coggins JR (1984) Planta 160: 78–83.

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant 15: 473–497.

    Google Scholar 

  • Padgette SR, Biest Re D, Gasser CS, Eichholtz DA, Frazier RB, Hironaka CM, Levine EB, Shah DM, Fraley RT, Kishore GM (1991) J. Biol. Chem. 266: 22364–22369.

    Google Scholar 

  • Peñaloza-Vázquez A, Herrera-Estrella L, Bailey AM 1994. Appl. and Environ. Microbiol. (submitted)

  • Powell HA, Kerby NW, Rowell P, Mousdale DM, Coggins JR (1992) Planta 188: 484–490.

    Google Scholar 

  • Sambrook J, Fritsch EF and Maniatis T (1989) Molecular cloning. A laboratory manual (Second ed.). New York: Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  • Schultz A, Wengenmayer F, Goodman M (1990) Critical Rev. of Plant Sci. 9: 1–15.

    Google Scholar 

  • Sellin C, Forlani G, Dubois J, Nielsen E, Vasseur J (1992) Plant. Sci. 85: 223–231.

    Google Scholar 

  • Shah DM, Horsch RB, Klee HJ, Kishore GM, Winter JA, Tumer NE, Hironaka CM, Sanders PR, Gasser CS, Aykent S, Siegel NR, Rogers SG, Fraley RT (1986) Science 233: 478–481.

    Google Scholar 

  • Smith PK, Krohn RI, Hermanson GT, Mallia AK, Gartner FH, Provenzano MD, Fujimoto EK, Goeke NM, Olson BJ, Klenk DC (1985) Anal. Biochem. 150: 76–85.

    Google Scholar 

  • Sost D, Amrhein N (1990) Arch. Biochem and Biophys. 282: 433–436.

    Google Scholar 

  • Tai Chun P, Hickok LG (1992) Can. J. Bot. 70: 1097–1099.

    Google Scholar 

  • van den Elzen PJM, Townsend J, Lee KY, Bedbrook JR (1985) Plant Mol. Biol. 5: 299–302.

    Google Scholar 

  • Velten J, Velten L, Hain R, Schell J (1984) EMBO J. 3: 2723–2730.

    Google Scholar 

  • Wang Y, Jones JD, Weller SC, Goldsbrough PB (1991) Plant Mol. Biol. 17: 1127–1138.

    Google Scholar 

  • Zemell RI, Anwar RA (1975) J. Biol. Chem. 250: 4959–4964.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Ingeniería Genética de Plantas, CINVESTAV-IPN Unidad Irapuato, Irapuato, Apdo. Postal 629, 36500, Gto., México

    Alejandro Peñaloza-Vázquez, Araceli Oropeza, Gilda L. Mena & Ana M. Bailey

Authors
  1. Alejandro Peñaloza-Vázquez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Araceli Oropeza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gilda L. Mena
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ana M. Bailey
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by I. K. Vasil

Rights and permissions

Reprints and permissions

About this article

Cite this article

Peñaloza-Vázquez, A., Oropeza, A., Mena, G.L. et al. Expression of the hygromycin B phosphotransferase gene confers tolerance to the herbicide glyphosate. Plant Cell Reports 14, 482–487 (1995). https://doi.org/10.1007/BF00232779

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00232779

Keywords

Search

Navigation