Summary
A method for the production of somatic embryos and subsequent plant regeneration for Anthurium andraeanum Linden ex André (Monocotyledonae) hybrids is described. Whole leaf blade explants, derived from plantlets grown in vitro, formed translucent embryogénic calli at their basal ends within one month of culture in the dark. Secondary somatic embryos formed frequently and without an intervening callus on surfaces of primary embryos. Embryogenesis was induced with three genotypes using a modified half-strength Murashige and Skoog (MS) medium supplemented with 1.0 to 4.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.33 to 1.0 mg l−1 kinetin. A combination of 2% sucrose with 1% glucose in the medium favored embryogenesis over 3% sucrose alone. Whole leaf blades on medium solidified with 0.18% Gelrite produced more somatic embryos than leaves on medium with 0.7% Bacto-agar. Within two to three months after culture initiation, embryos were transferred to modified MS medium containing 0.2 mg l−1 6-benzyladenine (BA) and 2% sucrose and placed in the light for conversion into plantlets. Rooted plantlets were recovered and transferred into pots with tree fern fiber medium and grown in the greenhouse.
Similar content being viewed by others
Abbreviations
- MS:
-
Murashige and Skoog (1962)
- 2,4-D:
-
2,4 dichlorophenoxyacetic acid
- BA:
-
6-benzyladenine
References
Ammirato PV (1983) In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 1, Macmillan, New York, pp 82–123
DeWald SG, Litz RE, Moore GA (1989) J Amer Soc Hort Sci 114:712–716
Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC (1990) Plant Cell Reports 9:189–194
Geier T (1982) In: Fujiwara A (ed) Plant tissue culture 1982. Japanese Association for Plant Tissue Culture, Tokyo, pp 137–138
Geier T (1990) In: Ammirato PV, Evans DA, Sharp WR. Bajaj YPS (eds) Handbook of plant cell culture, vol 5, McGraw-Hill, New York, 228–252
Ho WJ, Vasil IK (1983) Protoplasma 118:169–180
Kuehnle AR, Sugii N (1991a) HortScience 26:919–921
Kuehnle AR, Sugii N (1991b) HortScience 26:1325–1328
Kunisaki JT (1980) HortScience 15:508–509
Levi A. Sink KC (1990) J Plant Physiol 137:184–189
McGranahan GH, Leslie CA, Uratsu SL, Martin LA, Dandekar AM (1988) Bio/Technology 6:800–804
McGranahan GH, Leslie CA, Uratsu SL, Dandekar AM (1990) Plant Cell Reports 8:512–516
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Nishijima W (1988) In: Alvarez A (ed) Proceedings of the first anthurium blight conference, HITAHR 02.04.88, University of Hawaii, Honolulu, pp 6–8
Vasil V, Vasil IK (1982) Bot Gaz 143:454–465
Vasil V, Vasil IK (1984) In: Vasil IK (ed) Cell culture and somatic cell genetics of plants. Academic Press, Orlando, pp 738–743
Author information
Authors and Affiliations
Additional information
Communicated by J. J. Finer
Rights and permissions
About this article
Cite this article
Kuehnle, A.R., Chen, FC. & Sugii, N. Somatic embryogenesis and plant regeneration in Anthurium andraeanum hybrids. Plant Cell Reports 11, 438–442 (1992). https://doi.org/10.1007/BF00232686
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232686