Summary
The increased efficiency of embryogenic callus formation and regeneration of plantlets was the result of using immature induced polyembryos of wheat rather than single embryos as primary explants. Plant regeneration was effected via embryogenesis, organogenesis, and a combined process beginning as embryogenesis and proceeding as organogenesis.
Similar content being viewed by others
Abbreviations
- 2,4-D:
-
2,4 -dichlorophenoxyacetic acid
- MS:
-
Murashige and Skoog's medium (1962)
- PAR:
-
photosynthetically active radiation
References
Donovan GR, Lee JW (1977) Plant Sci Lett 9: 107–113
Fergusson JD, McEvan JM, Gard KA (1979) Physiol Plant 45: 470–474
He DG, Yang YM, Scott KJ (1988) Plant Sci 57: 225–233
He DG, Yang YM, Bertram J, Scott KJ (1990) Plant Sci 68: 103–111
Lhotová M, Kučera L (1990) Genet a šlecht 26: 257–269
Linacero R, Vazquez AM (1992) Plant Cell Rep 12: 26–28
Lörz H, Göbel E, Brown P (1988) Plant Breeding 100: 1–25
Maddock SE (1985) in: Bright SWJ and Jones MGK (eds) Cereal tissue and cell culture, Martinus Nijhoff, Dr W JunkPublishers, Dodrecht, pp 131–174
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Němec B (1962) Botanická mikrotechnika, Nakladatelství ČSAV, Praha, pp 482
Ozias-Akins P, Vasil JK (1982) Protoplasma 110: 95–105
Singh SK, Jenner CF (1983) Austr J Plant Physiol 10: 227–236
Vasil JK (1987) J Plant Physiol 128: 193–218
Author information
Authors and Affiliations
Additional information
Communicated by N. Amrhein
Rights and permissions
About this article
Cite this article
Erdelská, O., Vidovencová, Z. & Erdelský, K. Cleavage polyembryos as explants for plant regeneration in wheat. Plant Cell Reports 15, 342–344 (1996). https://doi.org/10.1007/BF00232368
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF00232368