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Clonal propagation of Virginia Pine (Pinus virginiana Mill.) by organogenesis

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Abstract

Clonal propagation of Virginia Pine (Pinus virginiana Mill.) was achieved by organogenesis on cotyledon explants. The influence of several cytokinins and abscisic acid on adventitious shoot production from cotyledon explants was investigated. Benzyladenine was more effective in shoot induction than three other cytokinins tested. Benzyladenine (22.2 μM) in combination with naphthaleneacetic acid(0.05 μM) in a Gresshoff and Doy (1972) medium was found to increase shoot bud production. Abscisic acid (7.6 μM) in combination with benzyladenine and naphthaleneacetic acid enhanced shoot formation by an additional 65%. Root initiation was achieved with 0.5 strength Gresshoff and Doy media amended with naphthaleneacetic acid (1.3 μM), indole-3-butyric acid (1.2 μM) and benzyladenine (0.4 μM). Over 2400 plantlets from 2 families survived and were transferred to a greenhouse in preparation for field planting. After ten months, the maximum number of surviving plantlets/seed explant from these two sources was 57 for family ALPV-38 and 41 for family ALPV-78, respectively.

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Abbreviations

ABA:

Abscisic acid

BA:

N6-Benzyladenine

GD:

Gresshoff and Doy (1972) nutrient media

IBA:

Indole-3-butyric acid

KN:

Kinetin

NAA:

α-Naphthaleneacetic acid

2iP:

2-isopentenyl adenine

ZN:

Zeatin

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Authors and Affiliations

  1. Department of Forest Science, Texas Agricultural Experiment Station, Texas A&M University System, 77843-2135, College Station, TX, USA

    S. Chang, S. Sen, J. Aimers-Halliday & R. J. Newton

  2. Department of Forest Science, Texas Forest Service, Texas A&M University System, 77843-2135, College Station, TX, USA

    C. R. McKinley

Authors
  1. S. Chang
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  2. S. Sen
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  3. C. R. McKinley
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  4. J. Aimers-Halliday
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  5. R. J. Newton
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Additional information

Communicated by G. C. Phillips

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Chang, S., Sen, S., McKinley, C.R. et al. Clonal propagation of Virginia Pine (Pinus virginiana Mill.) by organogenesis. Plant Cell Reports 10, 131–134 (1991). https://doi.org/10.1007/BF00232043

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  • DOI: https://doi.org/10.1007/BF00232043

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