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Cryopreservation of somatic embryos and embryonic axes of Camellia japonica L.

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Summary

Cryopreservation in liquid nitrogen was attempted with both somatic embryos and zygotic embryonic axes of the ornamental Camellia japonica L. Several protective measures were applied to somatic embryos (desiccation, chemical protectors, hardening by culture at low temperatures, encapsulation in alginate beads), but none allowed somatic embryos cultures to survive after 24 h in liquid nitrogen. Embryonic axes, however, were easily cryopreserved by means of the simplest technique: desiccation in a laminar flow hood and direct immersion in liquid nitrogen. Although the causes of the difference in cryopreservability between the two types of material are not known, one might be the difference between their degrees of differentiation and water content.

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Abbreviations

ANOVA:

analysis of variance

BA:

N6-benzyladenine

DMSO:

dimethyl sulfoxide

IBA:

indole-3-butyric acid

LN:

liquid nitrogen

MS:

Murashige and Skoog mineral solution

fwt:

fresh weight

LSD:

Least Significant Difference

References

  • Bercetche J, Galerne M, Dereuddre J (1990) C.R. Acad. Sci. Paris, 310 Série III: 357–363

    Google Scholar 

  • Chaudhury R, Radhamani J, Chandel KPS (1991) Cryo-Letters 12: 31–36

    Google Scholar 

  • de Boucaud MT, Brison M, Negier P (1994) Cryo-Letters 15: 151–160

    Google Scholar 

  • Dumet D, Engelmann F, Chabrillange N, Duval Y (1993) Plant Cell Reports 12: 352–355

    Google Scholar 

  • González-Benito ME, Pérez-Ruíz C (1994) Cryo-Letters 15: 41–46

    Google Scholar 

  • González-Benito ME, Pérez C (1994) Cryobiology 29: 685–690

    Google Scholar 

  • González-Río F, Gurriaran MJ, González E, Revilla MA (1994) Cryo-Letters 15: 337–342

    Google Scholar 

  • Hatanaka T, Yasuda T, Yamaguchi T, Sakai A (1994) Cryo-Letters 15: 47–52

    Google Scholar 

  • Kohmura H, Sakai A, Chokyu S, Yakuwa T (1992) Plant Cell Reports 11: 433–437

    Google Scholar 

  • Marin ML, Gogorcena Y, Ortiz J, Durán-Villa N (1993) Plant Cell Tissue Organ Culture 34:27–33

    Google Scholar 

  • Murashige T, Skoog F (1962) Physiol. Plant. 15: 473–497

    Google Scholar 

  • Niino T, Sakai A, Yakuwa H (1992) Cryo-Letters 13: 51–58

    Google Scholar 

  • Normah MN, Chin HF, Hor YL (1986) Pertanika 9:299–303

    Google Scholar 

  • Norman MN, Vengadasalam M (1992) Cryo-Letters 13: 199–208

    Google Scholar 

  • Pence VC (1992) Cryobiology 29: 391–399

    Google Scholar 

  • Tessereau H, Florin B, Meschine MC, Thierry C, Pétiard V (1994) Ann. Bot. 74: 547–555

    Google Scholar 

  • Vieitez AM, Bardela J (1990) Plant Cell Tissue Organ Culture 21: 267–274

    Google Scholar 

  • Vieitez, AM, San-José MC, Vieitez FJ, Ballester A (1991) J. Amer. Soc. Hort. Sci. 116:753–757

    Google Scholar 

  • Yamada T, Sakai A, Matsumura T, Higuchi S (1991) Plant Sci. 78: 81–87

    Google Scholar 

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Authors and Affiliations

  1. Instituto de Investigaciones Agrobiológicas de Galicia, CSIC, Apartado 122, 15080, Santiago de Compostela, Spain

    Laura V. Janeiro, Ana M. Vieitez & Antonio Ballester

Authors
  1. Laura V. Janeiro
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  2. Ana M. Vieitez
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  3. Antonio Ballester
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Communicated by H. Lörz

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Janeiro, L.V., Vieitez, A.M. & Ballester, A. Cryopreservation of somatic embryos and embryonic axes of Camellia japonica L.. Plant Cell Reports 15, 699–703 (1996). https://doi.org/10.1007/BF00231928

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  • DOI: https://doi.org/10.1007/BF00231928

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