Summary
The distribution and structural features of tyrosine hydroxylase-like immunoreactive (TH-LI) neurons were studied in the olfactory bulb of a snake, Elaphe quadrivirgata, by using pre-and post-embedding immunocytochemistry at the light microscopic level. In contrast to rodent olfactory bulbs previously reported, many TH-LI neurons were seen not only in the main olfactory bulb (MOB) but also in the accessory olfactory bulb (AOB). With regard to the TH-like immunoreactivity, there appeared no appreciable differences between MOB and AOB. As in mammalian MOB, the majority of TH-LI neurons were clustered in the periglomerular region and appeared to send their dendritic branches into glomeruli, which as a whole make an intense TH-LI band in the glomerular layer (GML). In the external plexiform/mitral cell layer (EPL/ML) of MOB and AOB as well as in the outer sublamina of the internal plexiform layer (OSL) of AOB, an appreciable number of TH-LI neurons were scattered, extending dendritic processes which appeared to make a loose meshwork. TH-LI neurons in EPL/ML (including OSL) appeared to consist of at least two morphologically different types. The first had a small perikaryon and one or two smooth dendrites which usually extended to GML and were frequently confirmed to enter into glomeruli. The second had a larger perikaryon and 2–3 dendrites which branched into several varicose processes extending in EPL/ML/OSL but appeared not to enter into glomeruli. The TH-like immunoreactivity was rarely seen in the internal plexiform layer and internal granule cell layer. The colocalization of GABA-like and TH-like immunoreactivities was further studied. Almost all TH-LI neurons in both EPL/ ML/OSL and GML contained GABA-like immunoreactivity irrespectively of the type of TH-LI cells.
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Abbreviations
- AOB:
-
accessory olfactory bulb
- MOB:
-
main olfactory bulb
- Hem:
-
hemisphere
- ON:
-
olfactory nerve layer
- VN:
-
vomeronasal nerve layer
- GM:
-
glomerular layer
- EP/M:
-
external plexiform layer/Mitral cell layer
- IP:
-
internal plexiform layer
- IG:
-
internal granular layer
- OS:
-
outer sublamina of the IPL of AOB
- MS:
-
middle sublamina of the IPL of AOB
- IS:
-
inner sublamina of the IPL of AOB
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Kosaka, T., Kosaka, K. & Nagatsu, I. Tyrosine hydroxylase-like immunoreactive neurons in the olfactory bulb of the snake, Elaphe quadrivirgata, with special reference to the colocalization of tyrosine hydroxylase- and GABA-like immunoreactivities. Exp Brain Res 87, 353–362 (1991). https://doi.org/10.1007/BF00231852
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DOI: https://doi.org/10.1007/BF00231852