Abstract
This report describes rapid regeneration of green plants from microspores of the barley cultivar Igri. Use of 0.3 M mannitol during maceration and isolation was essential for response from mechanically isolated microspores of barley cv. Igri grown under our conditions. A shed microspore culture system proved to be simple and gave a fast response; plants were obtained as early as 25 days after the material was taken from the donor plant. A 28-day cold-pretreatment of spikes can also be replaced with a 3–4 day pretreatment of anthers in mannitol. Shed microspores from 100 anthers produced an average of 292 plants with 91% of them green. Approximately 80% of the regenerated plants were spontaneously doubled-haploids.
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Abbreviations
- IAA:
-
Indole-3-acetic acid
- FHG:
-
Hunter's media (1988)
- MS:
-
Murashige and Skoog
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Communicated by E. D. Earle
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Ziauddin, A., Simion, E. & Kasha, K.J. Improved plant regeneration from shed microspore culture in barley (Hordeum vulgare L.) cv. igri. Plant Cell Reports 9, 69–72 (1990). https://doi.org/10.1007/BF00231551
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DOI: https://doi.org/10.1007/BF00231551