Summary
Results on the anatomy of ventromedial hypothalamic outputs, together with the temporal aspects of electrophysiological studies, predict that for reproductive behavior control sex steroids alter hypothalamic protein synthesis and transport of proteins to the dorsal midbrain. We have studied labeled proteins arriving in the dorsal midbrain after local microinjection of tritiated amino acids to the ventromedial hypothalamus. Estrogen treated and control ovariectomized female rats are significantly different in this respect, and in particular some proteins appear to be synthesized or transported in greater amounts in the estrogen treated animals. Physical characterization of these proteins and comparisons under a variety of endocrine conditions will suggest whether their synthesis could be part of the mechanism by which ovarian steroids affect behavior.
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References
Barnea A, Gorski J (1970) Estrogen-induced protein. Time course of synthesis. Biochemistry 9: 1899–1904
Cohen RS, Chung SK, Pfaff DW (1984) Estradiol causes nucleolar changes in hypothalamic nerve cells. Cell Tissue Res (in press)
Cohen R, Pfaff DW (1981) Ultrastructure of neurons in the ventromedial nucleus of the hypothalamus in ovariectomized rats with or without estrogen treatment. Cell Tissue Res 217: 451–470
DeAngelo AB, Gorski J (1970) Role of RNA synthesis in the estrogen induction of a specific uterine protein. Natl Acad Sci 66: 693–700
Finlay TH, Katz J, Kadner S, Levitz M (1984) Properties of an estrogen-induced hydrolytic enzyme from mouse uterus. J Steroid Biochem (in press)
Finlay TH, Katz J, Levitz M (1982) Purification and properties of an estrogen-stimulated hydrolase from mouse uterus. J Biol Chem 257: 10914–10919
Harlan RE, Shivers BD, Row L-M, Pfaff DW (1982) Intrahy-: pothalamic chochicine infusions disrupt lordotic responsiveness in estrogen-trated female rats. Brain Res 238: 153–167
Harlan R, Shivers B, Pfaff DW (1983) Midbrain microinfusions of prolactin increase the estrogen-dependent behavior, lordosis. Science 219: 1451–1453
Krieger MS, Conrad LCA, Pfaff DW (1979) An autoradiographic study of the efferent connections of the ventromedial nucleus of the hypothalamus. J Comp Neurol 183: 785–816
McEwen BS, Biegon A, Fischette C, Luine V, Parsons B, Rainbow T (1983) Toward a neurochemical basis of steroid hormone action. In: Martini L, Ganong WF (eds) Frontiers in neuroendocrinology. Raven Press, New York
O'Malley BW, McGuire WL, Kohler PO, Koreman SG (1969) Recent Prog Horm Res 25: 105
Pfaff DW (1980) Estrogens and brain function: neural analysis of a hormone-controlled mammalian reproductive behavior. Springer, Berlin Heidelberg New York
Pfaff DW (1984) Impact of steroid hormones on hypothalamic neurons: chemical, electrical and ultrastructural studies. Recent Prog Horm Res (in press)
Quadagno D, Ho G (1975) Horm Behav 6: 19
Schacter B, Shivers B, Harlan R, Pfaff D (1984) Prolactin gene is expressed in hypothalamic neurons. Endocrinology (in press)
Terkel A, Shryne J, Gorski R (1973) Horm Behav 4: 377
Vertes M, Kornyei J, Nagy L, Vertes Z, Kovacs S (1981) Stimulation by oestradiol of soluble-protein synthesis in rat hypothalamus. Mol Cell Endocrinol 22: 329–338
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Pfaff, D., Rosello, L. & Blackburn, P. Proteins synthesized in medial hypothalamus and transported to midbrain in estrogen-treated female rats. Exp Brain Res 57, 204–207 (1984). https://doi.org/10.1007/BF00231148
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DOI: https://doi.org/10.1007/BF00231148