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On the mechanism of estrogen receptor replenishment: recycling, resynthesis and/or processing

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Summary

Estrogen receptor replenishment has been extensively studied after a single injection of estradiol-17β in the rat. Most studies indicate that replenishment, under these conditions, is due both to recycling and to resynthesis of receptor. In the case of short-acting estrogens, total replenishment occurs in the absence of protein synthesis and loss of nuclear receptor closely corresponds to an increase in cytoplasmic receptor. After estradiol-17β injection, there is a loss of nuclear receptor without a corresponding increase in cytoplasmic receptor, leading to a loss in total receptor content or `processing'. Since little processing occurs with the active, short-acting estrogen, we propose that processing is not essential for estrogen action. Evidence is accumulating to indicate that processing may be due to a reversible inactivation of the steroid binding capacity of the receptor. We discuss a model in which there are two routes for replenishment: a simple equilibrium scheme where no processing occurs and a second route where the receptor is processed to a form with low affinity for estrogen which must be reactivated before binding can occur.

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  1. Departments of Biochemistry and Animal Science, University of Wisconsin, 53706, Madison, WI, U.S.A.

    Judy A. Kassis & Jack Gorski

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  1. Judy A. Kassis
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Kassis, J.A., Gorski, J. On the mechanism of estrogen receptor replenishment: recycling, resynthesis and/or processing. Mol Cell Biochem 52, 27–36 (1983). https://doi.org/10.1007/BF00230586

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