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Hypoglycemia-induced AP-1 transcription factor and basic fibroblast growth factor gene expression in multidrug resistant human breast carcinoma MCF-7/ADR cells

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Abstract

We investigated the effect of hypoglycemic treatment on the activation of the AP-1 transcription factors and the regulation of basic fibroblast growth factor (bFGF) gene expression in multidrug resistant human breast carcinoma MCF-7/ADR cells. Northern blot and gel mobility shift assays showed that hypoglycemic treatment induced c-jun and c-fos gene expression, AP-1 binding activity, as well as bFGF gene expression. Moreover, transfected cells expressing high levels of abnormal c-Jun protein exhibited a reduction in the bFGF protein levels compared to parental cells. A potent protein kinase C (PKC) inhibitor, H-7 (60 μg/ml) suppressed the stress-induced bFGF gene expression. Our study also demonstrated that H-7 did not facilitate the decay of bFGF mRNA. Thus, the suppression of bFGF gene expression by treatment with H-7 was due to the effect of the drug on the synthesis of bFGF mRNA rather than the stability of bFGF mRNA. Our data suggest that hypoglycemia-induced bFGF gene expression is mediated through the activation of PKC and the AP-1 transcription factors. (Mol Cell Biochem 155: 163–171, 1996)

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Authors and Affiliations

  1. Department of Radiation Oncology Research Laboratories, William Beaumont Hospital, 3601 W. Thirteen Mile Road, 48073, Royal Oak, MI, USA

    Sandra S. Galoforo, Christine M. Berns, Geza Erdos, Peter M. Corry & Yong J. Lee

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  1. Sandra S. Galoforo
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  2. Christine M. Berns
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  3. Geza Erdos
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  4. Peter M. Corry
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Galoforo, S.S., Berns, C.M., Erdos, G. et al. Hypoglycemia-induced AP-1 transcription factor and basic fibroblast growth factor gene expression in multidrug resistant human breast carcinoma MCF-7/ADR cells. Mol Cell Biochem 155, 163–171 (1996). https://doi.org/10.1007/BF00229313

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