Summary
The neurophysin that is biosynthesised in association with the neurohypophysial hormone vasopressin (vasopressin-neurophysin) affects the growth and DNA synthesis of rat hypothalamic non-neuronal cells in culture. Over a narrow range of concentrations vasopressin-neurophysin stimulated growth, as assessed by increase in cell numbers, about five-fold, in conditions where fetal calf serum concentration was limiting (0.2% fetal calf serum). Maximum stimulation occurred in the presence of 20 to 30 ng vasopressin-neurophysin per ml of medium. DNA synthesis was increased by a factor of three in the presence of 30 ng vasopressin-neurophysin per ml of medium. At least two populations of non-neuronal hypothalamic cells were present in the cultures, and these were both affected by vasopressin-neurophysin.
This study allows the suggestion that neurophysin may be acting as a growth-regulating factor at its release site, playing a part in the interactions of neurones and glial cells in the hypothalamo-neurohypophysial system.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Antoniades HN, Williams LT (1983) Human platelet-derived growth factor: structure and function. Fed Proc 42:2630–2634
Breslow E (1979) Chemistry and biology of the neurophysins. Ann Rev Biochem 48:251–274
Brownstein MJ, Russell JT, Gainer H (1980) Synthesis, transport and release of posterior pituitary hormones. Science 207:373–378
Calker D van, Hamprecht B (1980) Effects of neurohormones on glial cells. Adv Cell Neurobiol 1:31–67
Eagle H (1955) Nutrition needs of mammalian cells in tissue culture. Science 122:501–504
Galabov P, Schiebler TH (1978) The ultrastructure of the developing neural lobe. Cell Tissue Res 189:313–329
González CB, Swann RW, Pickering BT (1981) Effects of tunicamycin on the hypothalamo-neurohypophysial system of the rat. Cell Tissue Res 217:199–210
Gospodarowicz D (1981) The extracellular matrix and the control of cell proliferation. In: Martin JB, Reichlin S, Bick KL (eds) Neurosecretion and brain peptides. Raven Press, New York, pp 243–261
Gospodarowicz D, Ill CR (1980) Do plasma and serum have different abilities to promote cell growth? Proc Natl Acad Sci USA 77:2726–2730
Ham RG (1981) Survival and growth requirements of nontransformed cells. In: Baserga R (ed) Handbook of experimental pharmacology Vol 57: Tissue growth factors. Springer, New York, pp 13–88
Hanson GR, Iversen PL, Partlow LM (1982a) Preparation and partial characterization of highly purified primary cultures of neurons and non-neuronal (glial) cells from embryonic chick cerebral hemispheres. Dev Brain Res 3:529–545
Hanson G, Partlow LM, Iversen PL (1982b) Neuronal stimulation of non-neuronal (glial) cell proliferation: lack of specificity between different regions of the nervous system. Dev Brain Res 3:547–555
Heldin C-H, Estermark B, Wasteson Å (1979) Platelet-derived growth factor: purification and partial characterization. Proc Natl Acad Sci USA 76:3722–3726
Higashida H, Kato T, Kano-Tanaka K, Okuya M, Miyake A, Tanaka T (1981) Proliferation and synapse formation of neuroblastoma × glioma hybrid cells: effect of glia maturation factor. Brain Res 214:287–299
Land H, Grez M, Ruppert S, Schmale H, Rehbein M, Richter D, Schütz G (1983) Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA. Nature 302:342–344
Land H, Schütz G, Schmale H, Richter D (1982) Nucleotide sequence of cloned cDNA encoding bovine arginine vasopressinneurophysin II precursor. Nature 295:299–303
Lim R, O'Connell MM (1982) Effect of glia maturation factor on glial fibrillary acidic protein and fibronectin: a comparative study on glioblasts and fibroblasts using immunofluorescence. Dev Brain Res 5:29–39
Mirsky R, Wendon LMB, Black R, Stolkin C, Bray D (1978) Tetanus toxin: a cell surface marker for neurones in culture. Brain Res 148:251–259
Morris JF, Cannata MA (1973) Ultrastructural preservation of the dense core of posterior pituitary neurosecretory granules and its implications for hormone release. J Endocrinol 57:517–529
Nicholson HD, Swann RW, Burford GD, Wathes DC, Porter DG, Pickering BT (1984) Identification of oxytocin and vasopressin in the testis and in adrenal tissue. Regul Pept 8:141–146
Norrgren G, Ebendal T, Belew M, Jacobson C-O, Porath J (1980) Release of nerve growth factor by human glial cells in culture. Exptl Cell Res 130:31–39
Palkovits M (1973) Isolated removal of hypothalamic or other brain nuclei of the rat. Brain Res 59:449–450
Pardee AB, Bubrow R, Hamlin JL, Kletzien RF (1978) Animal cell cycle. Ann Rev Biochem 47:715–750
Pickering BT (1978) The neurosecretory neurone: a model system for the study of secretion. Essays Biochem 14:45–81
Pickering BT, Swann RW (1983) Secretion in the posterior pituitary gland. In: Cantin M (ed) The secretory process. Karger, Basel, pp 247–275
Pickering BT, Jones CW, Burford GD, McPherson MA, Swann RW, Heap PF, Morris JF (1975) The rôle of neurophysin proteins: suggestions from the study of their transport and turnover. Ann New York Acad Sci 248:15–36
Pledger WJ, Hart CA, Wharton WR (1981) Mammalian cell proliferation is regulated by the synergistic actions of multiple growth factors. Adv Exp Med Biol 138:287–300
Pruss RM (1982) The potential use of cultured cells to detect nonneuronal targets of neuropeptide action. Peptides 3:231–233
Sinding C, Robinson AG, Seif SM, Schmid PG (1980) Neurohypophyseal peptides in the developing rat fetus. Brain Res 195:177–186
Sternberger LA, Hardy PH, Cuculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-anti horseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem 18:315–333
Swann RW, González CB, Birkett SD, Pickering BT (1982) Precursors in the biosynthesis of vasopressin and oxytocin in the rat. Characteristics of all the components in high performance liquid chromatography. Biochem J 208:339–349
Wallenstein S, Zucher CL, Fleiss JL (1980) Some statistical methods useful in circulation research. Circ Res 47:1–9
Whatley SA, Hall C, Lim L (1981) Hypothalamic neurons in dissociated cell culture: the mechanism of increased survival times in the presence of non-neuronal cells. J Neurochem 36:2052–2056
Worley RTS, González CB (1981) Cell culture of hypothalamic neurons from rats and amphibians. J Anat 132:459
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Worley, R.T.S., Pickering, B.T. Non-neuronal cells of rat hypothalamus in dissociated cell culture. Cell Tissue Res. 237, 161–168 (1984). https://doi.org/10.1007/BF00229212
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00229212