Summary
Specific antisera against protein-conjugated γ-aminobutyric acid (GABA) were used in immunocytochemical staining procedures to study the distribution of the putative GABA-like immunoreactive neurons in the optic lobes of Periplaneta. GABA-like immunoreactive structures are evident in all three optic neuropil regions. Six different populations of GABAergic neurons, whose perikarya are grouped around the medulla, are found within the optic lobe. The number of these immunoreactive cells varies greatly and corresponds to the number of ommatidia of the eye. In the proximal part of the lamina, a coarse network of GABA-positive fibres is recognizable. These are the processes of large field tangential cells whose fibres pass through the distal surface of the medulla. A second fibre population of the lamina is made up of the processes of the centrifugal columnar neurons whose perikarya lie proximally to the medulla. The medulla contains 9 layers with GABAergic elements of variable immunoreactivity. Layers 1, 3, 5, 7 and 9 exhibit strong labelling, as a result of partial overlapping of the processes of centrifugal and centripetal columnar neurons, tangential fibres and/or lateral processes of perpendicular fibres and (possibly) processes of amacrines. A strong immunoreactivity is found in the proximal and distal layers of the lobula.
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Blechschmidt K, Eckert M, Penzlin H (1988) Simultaneous localization of a classical neurotransmitter (GABA) and a peptide transmitter candidate (proctolin) in the nervous system of the cockroach, Periplaneta americana. Acta Histochem Suppl 35:127–135
Butler R (1973a) The anatomy of the compound eye of Periplaneta americana L. 1. General features. J Comp Physiol 83:223–238
Butler R (1973b) The anatomy of the compound eye of Periplaneta americana L. 2. Fine structure. J Comp Physiol 83:239–262
Butler R, Horridge GA (1972) The electrophysiology of the retina of Periplaneta americana L. l. Changes in receptor acuity upon light/dark adaption. J Comp Physiol 83:263–278
Butler R, Horridge GA (1973b) The electrophysiology of the retina of Periplaneta americana L. 2. Receptor sensitivity and polarized light sensitivity. J Comp Physiol 83:279–288
Datum KH, Weiler R, Zettler F (1986) Immunocytochemical demonstration of γ-amino butyric acid and glutamic acid decarboxylase in R7 photoreceptors and C2 centrifugal fibres in the blowfly visual system. J Comp Physiol A 159:241–249
Duve H, Thorpe A (1983) Immunochemical identification of vertebrate-type brain-gut peptides in insect nerve cells. In: Strausfeld NJ (ed) Functional Neuroanatomy. Springer, Berlin Heidelberg New York, pp 250–266
Eckert M, Johansson O, Hökfelt T, Blechschmidt K (1987) Characterization of the proctolinergic and GABAergic systems in the terminal ganglion of the cockroach, Periplaneta americana, by means of immunocytochemistry using computerized image analysis. J Chem Neuroanat (in press)
Ernst A, Füller H (1987) Zur Feinstruktur der Lamina ganglionaris von Periplaneta americana (L.). Zool Jb Anat 115:393–416
Homberg U, Kingan TG, Hildebrand JG (1987) Immunocytochemistry of GABA in the brain and suboesophageal ganglion of Manduca sexta. Cell Tissue Res 248:1–24
Hoskins SG, Homberg U, Kingan TG, Hildebrand JG (1985) Neurochcmical anatomy of the brain of the sphinx moth Manduca sexta. In: Neuropharmacology and pesticide action (Neurotox 85). Soc Chem Industry, London, pp 84–87
Hoskins SG, Homberg U, Kingan TG, Christensen TA, Hildebrand JG (1986) Immunocytochemistry of GABA in the antennal lobes of the sphinx moth Manduca sexta. Cell Tissue Res 244:243–252
Hüsing JO, Nitschmann J (1987) Lexikon der Bienenkunde. Edition Leipzig
Klemm N (1983) Detection of serotonin-containing neurons in the insect nervous system by antibodies to 5-HT. In: Strausfeld NJ (ed) Functional Neuroanatomy. Springer, Berlin Heidelberg New York, pp 302–316
Klemm N, Steinbusch HWM, Sundler F (1984) Distribution of serotonin containing neurons and their pathways in the supraoesophageal ganglion of the cockroach Periplaneta americana (L.) as revealed by immunocytochemistry. J Comp Neurol 225:387–395
Meyer EP, Matute C, Streit P, Nässel DR (1986) Insect optic lobe neurons identifiable with monoclonal antibodies to GABA. Histochemistry 84:207–216
Nässel DR, Klemm N (1983) Serotonin-like immunoreactivity in the optic lobes of three insect species. Cell Tissue Res 232:129–140
Nässel DR, Meyer EP, Klemm N (1985) Mapping the ultrastructure of serotonin-immunoreactive neurons in the optic lobe of three insect species. J Comp Neurol 232:190–204
Novel MS (1981) Postembryonic growth of the compound eye of the cockroach. J Embryol Exp Morphol 62:259–275
Nowikoff M (1932) Über den Bau der Komplexaugen von Periplaneta (Stylopyga) orientalis L. Jena Z Naturw 67:58–69
Ribi W (1977) Fine structure of the first optic ganglion (lamina) of the cockroach, Periplaneta americana. Tissue Cell 9:57–72
Schäfer S, Bicker G (1986) Distribution of GABA-like immunoreactivity in the brain of the honeybee. J Comp Neurol 246:287–300
Schürmann FW, Klemm N (1984) Serotonin-immunoreactive neurons in the brain of the honey bee. J Comp Neurol 225:570–580
Storm-Mathisen J, Leknes AK, Bore AT, Vaaland JL, Edminson P, Haug FS, Ottersen OP (1983) First visualization of glutamate and GABA in neurons by immunohistochemistry. Nature 301:517–520
Strausfeld NJ (1976) Atlas of an insect brain. Springer, Berlin Heidelberg New York
Trujillo-Cenóz O, Melamed J (1971) Spatial distribution of photoreceptor cells in the ommatidia of Periplaneta americana. J Ultrastruct Res 34:397–400
Tyrer NM, Turner JD, Altmann JS (1984) Identifiable neurons in the locust central nervous system that react with antibodies to serotonin. J Comp Neurol 227:313–330
Wolken JJ, Gupta PT (1961) Photoreceptor structures. The retinal cells of the cockroach eye. IV. Periplaneta americana and Blaberus giganteus. J Biophys Biochem Cytol 9:720–724
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Füller, H., Eckert, M. & Blechschmidt, K. Distribution of GABA-like immunoreactive neurons in the optic lobes of Periplaneta americana . Cell Tissue Res. 255, 225–233 (1989). https://doi.org/10.1007/BF00229085
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DOI: https://doi.org/10.1007/BF00229085