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Oxygen free radical producing activity of polymorphonuclear leukocytes in patients with Parkinson's disease

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Abstract

Oxygen free radicals (OFRs) have been suggested in the pathogenesis of Parkinson's disease (PD). These free radicals exert their cytotoxic effect by peroxidation of lipid membrane resulting in the formation of malondialdehyde (MDA). Polymorphonuclear (PMN) leukocyte is one of the major sources of OFR. However, the oxygen free radical producing activity of PMN leukocytes in patients with PD is not known. We therefore studied the oxygen free radical producing activity of polymorphonuclear leukocytes and MDA levels in the serum of healthy subjects and in patients with Parkinson's disease. The oxygen free radical producing activity of PMN leukocytes in blood and the MDA content in serum were significantly higher in patients with Parkinson's disease than in healthy subjects. These results indicate a possible role of oxygen free radicals in the pathogenesis of Parkinson's disease.

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References

  1. Calne DB, Eisen A, McGeer E et al.: Alzheimer's disease, Parkinson's disease, and motorneuron disease: abiotrophic interactions between aging and environment? Lancet ii: 1067–1070, 1986

    Google Scholar 

  2. Barbeau A: Etiology of Parkinson's disease: A research strategy. Can J Neurol Sci 11: 24–28, 1984

    Google Scholar 

  3. McGeer PL, McGeer EG, Suzuki JS: Aging and extrapyramidal function. Arch Neurol 34: 33–35, 1977

    CAS  PubMed  Google Scholar 

  4. Thiessen B, Rajput AH, Laverty W et al.: Age, environments, and the number of substantia nigra neurons. In: MB Streifler, E Melamed, AD Korczyn et al. (Eds): Advances in Neurology, Vol 53 Parkinson's disease: Anatomy, Pathology and Therapy. Raven Press, New York 1990; pp 201–206

    Google Scholar 

  5. Cohen G: The pathobiology of Parkinson's disease: biochemical aspects of dopamine neuron senescence. J Neural Transm 19(Suppl): 89–103, 1983

    CAS  PubMed  Google Scholar 

  6. Burns RS, Chiueh CC, Markey SP et al.: A primate model of parkinsonism: Selective destruction of dopaminergic neurons in the pars compacta of the substantia nigra by N-methyl-4-phenyl-1,2,3,6-tetra-hydropyridine. Proc Natl Acad Sci USA 80: 4545–4550, 1983

    Google Scholar 

  7. Forno LS, Langston JW, Delanney LE: Locus Cereuleus lesions and eosinophilic inclusions in MPTP-treated monkeys. Ann Neurol 20: 449–454, 1986

    CAS  PubMed  Google Scholar 

  8. Ballard PA, Tetrud JW, Langston JW: Permanent human parkinsonism due to 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP): Seven Cases. Neurology 35: 949–956, 1985

    Google Scholar 

  9. Kopin IJ, Markey SP, Burns RS et al.: Mechanisms of neurotoxicity of MPTP. In: S Fahn, CD Marsden, P Jenner, P Teychenne (Eds): Recent Developments in Parkinson's disease, Raven Press, New York 1985; pp 165–173

    Google Scholar 

  10. Rossetti ZL, Sotgiu A, Sharp DE et al.: 1-methyl-4-pheny1–1,2,3,6-tetrahydropyridine and free radicals in vitro. Biochem Pharmacol 37: 4573–4574, 1988

    Google Scholar 

  11. Prasad K, Kalra J: Experimental atherosclerosis and oxygen free radical. Angiology 40: 835–843, 1989

    Google Scholar 

  12. McCord JM: Oxygen-drived free radicals in post ischemic tissue injury. N Engl J Med 312: 159–163, 1985

    Google Scholar 

  13. Prasad K, Kalra J, Buchko G: Acute hemorrhage and oxygen free radicals. Angiology 39: 1005–1013, 1988

    Google Scholar 

  14. Chan PH, Schmidley JW, Fishman RA et al.: Brain injury, edema and vascular permeability changes induced by oxygen-derived free radicals. Neurology 34: 315–320, 1984

    Google Scholar 

  15. Prasad K, Kalra J, Chaudhary AK et al.: Effect of Polymorphonuclear leukocyte-derived oxygen free radicals and hypochlorous acid on cardiac function and some biochemical parameters. Am Heart J 119: 538–550, 1990

    CAS  PubMed  Google Scholar 

  16. Babior BM: The respiratory burst of phagocytes. J Clin Invest 73: 599–601, 1984

    Google Scholar 

  17. Meerson FZ, Kagon VE, Kozlov YP et al.: The role of lipid peroxidation in pathogenesis of ischemic damage and antioxidant production of the heart. Basic Res Cardiol 77: 465–485, 1982

    Google Scholar 

  18. Hochstein P, Jain SK: Association of lipid peroxidation and polymerization of membrane proteins with erythrocyte aging. Fed Proc 40: 183–188, 1981

    Google Scholar 

  19. Wallace LKB: 1251 Luminometer Manual 1985

  20. Prasad K, Kalra J, Bharadwaj B: Increased chemiluminescence of polymorphonuclear leukocytes in dogs with volume overload heart failure. Brit J Exp Pathol 70: 463–468, 1989

    Google Scholar 

  21. Tono-Oko T, Veno N, Matsumoto T: Chemiluminescence of whole blood. A simple and rapid method for the estimation of phagocytic function of granulocytes and opsonic activity in whole blood. Clin Immun Immunopath 26: 66–75, 1983

    Google Scholar 

  22. Yagi K: A simple fluorimetric assay for lipid peroxides in blood plasma. Biochem Med 15: 212–216, 1976

    Google Scholar 

  23. Hastings MJG, Pretricevic I, Williams AJ et al.: The effect of culture media on the production and measurement of luminol-dependent chemiluminescence. Brit J Exp Path 63: 147–153, 1982

    Google Scholar 

  24. Holt ME, Ryall MET, Campbell AK: Albumin inhibits human polymorphonuclear leukocyte luminol-dependent chemiluminescence: evidence for oxygen free radical scavenging. Brit J Exp Path 65: 231–241, 1984

    Google Scholar 

  25. Nelson RD, Herron MJ, Schmidtke JR et al.: Chemiluminescence response of human leukocytes: Influence of medium components on light production. Infection Immun 17: 513–520, 1977

    Google Scholar 

  26. Dechatelet CR, Chirley PS: Evaluation of chronic granulomatous diseases by a chemiluminescent assay of microlitre quantities of whole blood. Clin Chem 27: 1739–1741, 1981

    Google Scholar 

  27. Selvaraj RJ, Sbarra AJ, Thomas GB et al.: A microtechnique for studying chemiluminescence response of phagocytes using whole blood and its application to the evaluation of phagocytes in pregnancy. J Reticuloendothel Soc 31: 3–16, 1982

    Google Scholar 

  28. Kalra J, Cunningham TA, Prasad K: Superoxide anion production by human phagocytes in blood. Variations with Aging [Abstract]. Clin Biochem 22: 257, 1989

    Google Scholar 

  29. Kalra J, Chaudhary AK, Cunningham TA et al.: Effect of aging on oxygen free radicals producing activity of polymorphonuclear leukocytes [Abstract]. Age 11: 176, 1988

    Google Scholar 

  30. Ford-Hutchinson AW, Bray MA, Doig MV et al.: Leukotriene B4, a potent chemokinetic and aggregating substance released from polymorphonuclear leukocytes. Nature 286: 264–267, 1980

    Google Scholar 

  31. Fantone JC, Ward PA: Role of oxygen-derived free radicals and metabolites in leukocyte dependent inflammatory reactions. Am J Pathol 107: 397–418, 1982

    Google Scholar 

  32. Spina MB, Cohen G: Dopamine turnover and glutathione oxidation: Implications for Parkinson's disease. Proc Natl Acad Sci USA 86: 1398–1400, 1989

    CAS  PubMed  Google Scholar 

  33. Dexter D, Carter C, Agid F et al.: Lipid peroxidation as cause of nigral cell death in Parkinson's disease. Lancet i 639–640, 1986

    Google Scholar 

  34. Mullane KM, Salmon JA, Kraemer R: Leukocyte-derived metabolites of arachidonic acid in ischemia-induced myocardial injury. Fed Proc 46: 2422–2433, 1987

    Google Scholar 

  35. Hernandez LA, Grisham MB, Twohig B et al.: Role of neutrophils in ischemia-reperfusion-induced microvascular injury. Am J Physiol 253: H699-H703, 1987

    Google Scholar 

  36. Ross R: The pathogenesis of atherosclerosis; an update. N Engl J Med 314: 488–500, 1986

    Google Scholar 

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Authors and Affiliations

  1. Departments of Pathology, Medicine (Neurology) and Physiology, College of Medicine, University of Saskatchewan and Royal University Hospital, S7N 0X0, Saskatoon, Saskatchewan,, Canada

    Jawahar Kalra, Ali H. Rajput, Subrahmanyam V. Mantha, Ajai K. Chaudhary & Kailash Prasad

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  1. Jawahar Kalra
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  2. Ali H. Rajput
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  3. Subrahmanyam V. Mantha
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  4. Ajai K. Chaudhary
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  5. Kailash Prasad
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Kalra, J., Rajput, A.H., Mantha, S.V. et al. Oxygen free radical producing activity of polymorphonuclear leukocytes in patients with Parkinson's disease. Mol Cell Biochem 112, 181–186 (1992). https://doi.org/10.1007/BF00227575

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  • DOI: https://doi.org/10.1007/BF00227575

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