Summary
In untreated adult male albino rats nuclear volume and the percentage of binucleate cells were determined in the first layer of hepatocytes adjacent to hepatic venous branches of varying diameters (<40 μm, 40 μm–80 μm, 80 μm–120μm, 120 μm–160 μm, >160 μm), and in the third and fourth layer of hepatocytes in the remainder of the perivenous parenchyma. In the first layer of hepatocytes adjacent to the vascular structures means of nuclear volume are significantly lower and percentage of binucleate cells significantly higher than in the cells of the remainder of the perivenous parenchyma. Within each area measured distribution curves of nuclear volume classes were homogeneous but showed heterogeneity in comparison with each other. The morphometric data presented in this study strongly support the opinion of the heterogeneity of liver cells in the perivenous zone, as previously postulated on the basis of histochemical investigations.
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References
Armitage P (1980) Statistical methods in medical research. Blackwell, Oxford London Edinburgh Boston Melbourne
Eichner D (1963) Zur Rationalisierung der Vermessung und Registrierung von Zellkernen. Z Mikrosk Anat Forsch 69:381–387
Elias H, Sherrick JC (1969) Morphology of the liver. Academic Press, New York London
Glaser WR (1978) Varianzanalyse. Fischer, Stuttgart, New York
Gumucio JJ, Miller DL, Krauss MD, Zanolli CC (1981) Transport of fluorescent compounds into hepatocytes and the resultant zonal labeling of the hepatic acinus in the rat. Gastroenterology 80:639–646
Hildebrand R (1980) Nuclear volume and cellular metabolism. Adv Anat Embryol Cell Biol 60:1–54
Hildebrand R, Wegener E (1980) Beitrag zu rationellen Vermessung von Zellkernvolumina. Gegenbaurs morph Jahrb 126:965–968
Inke G, Palkovits M, Gyarfas J (1958) Über methodische Fragen der Kernvariationsstatistik. VI. Bewertung der Gruppierungsmethoden. Acta Morphol Acad Sci Hung 8:263–271
Jerusalem Ch, Eling W, Jap P (1970) Histochemische und elektronenmikroskopische Veränderungen der Leberzelle im Tagesrhythmus und unter experimentellen Bedingungen. Acta Histochem (Jena) 36:168–206
Jungermann K, Katz N (1982) Functional hepatocellular heterogeneity. Hepatology 2:385–395
Köpf-Maier P, Wüstenfeld E (1975) Über das rhythmische Wachstum adäquat gereizter Ganglienzellen der Nuclei acustici (Ratte). Anat Anz 137:345–356
Le Bouton AV (1976) DNA-synthesis and cell proliferation in the simple liver acinus of 10 to 20-day-old rats: Evidence for cell fusion. Anat Rec 184:679–688
Nadal C, Zajdela F (1966) Polyploïdie somatique dans le foie de rat. I. Le rôle des cellules binnucléées dans la genèse des cellules polyploïdes. Exp Cell Res 42:99–116
Palkovits M (1961) Angaben und Hilfsmittel zur Auswertung von Kernvariationsuntersuchungen. Z mikr anat Forsch 67:343–355
Palkovits M, Fischer J (1968) Karyometric investigations. Akadémiai Kiadó, Budapest
Pearse AGE (1968) Histochemistry, theoretical and applied, Vol 1. Churchill, London
Rappaport AM, Borowy ZJ, Lougheed WM, Lotto WN (1954) Subdivision of hexagonal liver lobules into a structural and functional unit. Anat Rec 119:11–33
Rastl P, Fasching W (1974) Auftrennung von zweigipfeligen Zellkerngrößen-Verteilungskurven am Beispiel der Rattenleber. Z Anat Entwickl-Gesch 145:219–222
Rieder H (1981) NADP-dependent dehydrogenases in rat liver parenchyma. III. The description of a liponeogenic area on the basis of histochemically demonstrated enzyme activities and the neutral fat content during fasting and refeeding. Histochemistry 72:579–615
Schultz M, Hildebrand R (1983) Karyometric investigation on circadian rhythmic changes in the periportal and perivenous zone of the acinus of the rat liver. Cell Tissue Res 231:643–654
Teutsch HF (1981) Chemomorphology of liver parenchyma. Progr Histochem Cytochem 14:1–92
Wheatly DN (1972) Binucleation in mammalian liver. Studies on the control of cytokinesis in vivo. Exp Cell Res 74:455–465
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Supported by the Deutsche Forschungsgemeinschaft (Hi 318/2-1)
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Hildebrand, R., Karcher, B. Karyometry of nuclei in liver cells. Cell Tissue Res. 235, 669–673 (1984). https://doi.org/10.1007/BF00226967
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DOI: https://doi.org/10.1007/BF00226967