Summary
Alpha-1-antitrypsin immunoreactivity was demonstrated by immunofluorescence technique in the peripheral islet cells of all ten normal adult human pancreata examined; normal adult human liver was negative. The specificity of the reactions was confirmed by applying various control tests including absorption of the specific antisera with purified alpha-1-antitryspin, inhibition and blocking tests and by ensuring the monospecificity of the antisera used. The findings suggest that the pancreatic islet may be an additional source of alpha-1-antitryspin.
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The authors acknowledge the valuable help of Dr. P. Goddeeris and thank Bernadette Smets for technical assistance. Mr.M. Rooseleers for preparing photographs and Mrs. M. Vandenreyken for typing the manuscript. We are grateful to Professor S. Eriksson and Dr. R.E. Chance for providing antialpha-1-antitrypsin and pancreatic polypeptide respectively
This study is supported by grants from the Nationaal Fonds voor Wetenschappelijk onderzoek of Belgium
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Ray, M.B., Desmet, V.J. & Gepts, W. Alpha-1-antitrypsin immunoreactivity in islet cells of adult human pancreas. Cell Tissue Res. 185, 63–68 (1977). https://doi.org/10.1007/BF00226668
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DOI: https://doi.org/10.1007/BF00226668