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Grain protein variability among populations of wild barley (Hordeum spontaneum C. Koch.) from Jordan

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Summary

Protein content, kernel weight, and genetic diversity in the storage protein hordein, encoded by the Hor 1 and Hor 2 loci, were assessed in 12 populations of wild barley (Hordeum spontaneum C. Koch.) collected from central, peripheral, and marginal areas of its distribution in Jordan. Protein content ranged from 106.3 to 239.1 g kg-1, and kernel weight ranged from 21.17 to 31.8 mg. Populations with high protein content and heavy kernels have been identified. Electrophoretic analysis of the storage protein hordein showed that the two hordein loci, Hor 1 and Hor 2, are highly polymorphic, having 34 and 38 alleles, respectively. Polymorphism (He) was highest in central populations (He Hor 1=0.859, He Hor 2=0.782), intermediate in peripheral populations (He Hor 1=0.566, He Hor 2=0.509), and lowest in marginal populations (He Hor 1=0.392, He Hor 2=0.349). Geographical distances between populations were not indicative of Nei's genetic similarity (NI). NI values averaged 0.209 and ranged from 0.0 to 0.83, supporting the hypothesis of an island population model for the species. The high proportion of allelic diversity, apportioned among populations for Hor 1 (0.584) and Hor 2 (0.495) loci, indicates that these natural populations are a rich reserve of genetic variability for protein. This variability is readily exploitable in breeding.

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References

  • Ahokas H (1982) Variation of kernel protein and lysine in the wild progenitor of barley. Hereditas 96:29–37

    Google Scholar 

  • Britting PK, Goodman MM (1989) Genetic variation in crop plants and management of germplasm collections. In: Stalker HT, Chapman C (eds) Scientific management of germplasm: characterization, evaluation and enhancement. IBPGR and North Carolina State University, Rome, pp 41–53

    Google Scholar 

  • Brown AHD, Zohary D, Nevo E (1978) Outcrossing rates and heterozygosity in natural populations of Hordeum spontaneum Koch from Israel. Heredity 41:49–62

    Google Scholar 

  • Corke H, Atsmon D (1990) Wild barley (Hordeum spontaneum C. Koch.) and its potential utilization in barley protein improvement. Isr J Bot 39:271–286

    Google Scholar 

  • Doll H, Andersen B (1981) Preparation of barley storage protein, hordein, for the analytical sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal Biochem 115:61–66

    Google Scholar 

  • Doll H, Brown AHD (1979) Hordein variation in wild (Hordeum spontaneum) and cultivated (Hordeum vulgare L.) barley. Can J Genet Cytol 21:391–404

    Google Scholar 

  • Jana S, Pietrzak LN, Morris MI, Srivastava JP, Holwerda BC, Thai KM (1987a) Genetic diversity in wild barley (Hordeum spontaneum) populations of the Fertile Crescent. In: Yasuda S, Konishi T (eds) Barley genetics V. Proc 5th Int Barley Genet Symp, Okayama, 1986, pp 63–73

  • Jana S, Pietrzak LN, Jaradat AA (1987b) Assessment of diversity in wild and cultivated barleys of the Fertile Crescent: a new outlook. In: Yasuda S, Konishi T (eds) Barley genetics V. Proc 5th Int Barley Genet Symp, Okayama, 1986, pp 103–107

  • Jaradat AA, Jaradat TT, Jana S, Srivastava JP (1987) Diversity for quantitative characters in Jordanian landraces of barley. In: Yasuda S, Konishi T (eds) Barley genetics V. Proc 5th Int Barley Genet Symp, Okayama, 1986, pp 109–117

  • Kreis M, Shewry PR, Forde BG, Rahman S, Miflin BJ (1983) Molecular analysis of mutations conferring the high-lysine phenotype on the grain of barley (Hordeum vulgare L.) Cell 34:161–167

    Google Scholar 

  • Levi AA, Feldman M (1988) Ecogeographical distribution of HMW glutenin alleles in populations of the wild tetraploid wheat Triticum turgidum var. dicoccoides. Theor Appl Genet 75:651–658

    Google Scholar 

  • Margiotta B, Lafiandra D, Tomassini C, Perrino P, Porceddu E (1988) Variation in high molecular weight glutenin subunits in a hexaploid wheat collection from Nepal. In: Miller TE, Koebner RMD (eds) Proc 7th Int Wheat Genet Symp, Cambridge, Institute of Plant Scientific Research (IPSR), Cambridge, pp 975–980

    Google Scholar 

  • National Atlas of Jordan (1984) Climate and agroclimatology. Part I. Jordan National Geographic Center, Amman, pp 135

    Google Scholar 

  • Nei M (1972) Genetic distances between populations. Am Nat 106:283–292

    Article  Google Scholar 

  • Nei M (1973) Analysis of genetic diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323

    Google Scholar 

  • Nevo E, Payne PI (1987) Wheat storage proteins: diversity of HMW glutenin subunits in wild emmer from Israel. 1. Geographical patterns and ecological predictability. Theor Appl Genet 74:827–836

    Google Scholar 

  • Nevo E, Atsmon D, Beiles A (1985) Protein resources in wild barley (Hordeum spontaneum) in Israel: predictive method by ecology and allozyme markers. Plant Syst Evol 150:205–222

    Google Scholar 

  • Nevo E, Beiles A, Storch N, Doll H, Andersen B (1983) Microgeographic edaphic differentiation in hordein polymorphism of wild barley. Theor Appl Genet 68:123–132

    Google Scholar 

  • Nevo E, Grama A, Golenberg EM (1986) Protein resources in wild wheat, Triticum dicoccoides, in Israel: predictive method by ecology and allozyme markers. Genetica 68:215–228

    Google Scholar 

  • Newman CW, McGuire CF (1985) Nutritional quality. In: Rasmusson (ed) Barley. ASA, CSSA, SSSA, Madison, WI, pp 403–456

    Google Scholar 

  • Schonewald-Cox C, Chambers S, MacBryde B, Thomas L (1983) Genetics and conservation. Benjamin-Cumings, Menlo Park/CA, 722 pp

    Google Scholar 

  • Shewry PR, Williamson MS, Parmer S, Burgess SR, Buxton B, Kreis M (1987) The biochemical and molecular genetics of barley seed proteins. In: Yasuda S, Konishi T (eds) Barley genetics V. Proc 5th Int Barley Genet Symp, Okayama, pp 433–443

  • Smith-Huerta NL, Huerta AJ, Barnhart D, Waines JG (1989) Genetic diversity in wild diploid wheats Triticum monococcu var. boeoticum and Triticum urartu (Poaceae). Theor Appl Genet 78:260–264

    Google Scholar 

  • Soave C, Salamini F (1983) Genetic organization and regulation of maize storage proteins. In: Daussand J, Mosse J, Vaughan J (eds) Seed proteins. Academic Press, London, pp 205–218

    Google Scholar 

  • Sozinov AA, Pomortsev AA, Netsvetaev VP (1987) Blocks of hordein components as genetic markers. In: Yasuda S, Konishi T (eds) Barley genetics V. Proc 5th Int Barley Genet Symp, Okayama, pp 887–894

  • Spagnolleti-Zeuli PL, DePace C, Porceddu E (1984) Variation in durum wheat populations from three geographical origins. I. Material and spike characteristics. Euphytica 33:563–575

    Google Scholar 

  • Tsuchiya T (1987) Gene analysis and linkage studies in barley. In: Yasuda S, Konishi T (eds) Barley genetics V. Proc 5th Int Barley Genet Symp, Okayama, 1986, pp 189–199

  • Wilkinson L (1988) SYGRAPH. SYSTAT, Inc., Evanston/IL

    Google Scholar 

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  1. Department of Agricultural Sciences, Jordan University of Science and Technology, P.O. Box 3030, Irbid, Jordan

    A. A. Jaradat

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  1. A. A. Jaradat
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Communicated by J. Mac Key

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Jaradat, A.A. Grain protein variability among populations of wild barley (Hordeum spontaneum C. Koch.) from Jordan. Theoret. Appl. Genetics 83, 164–168 (1991). https://doi.org/10.1007/BF00226246

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  • DOI: https://doi.org/10.1007/BF00226246

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