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Histone complements of human tissues, carcinomas, and carcinoma-derived cell lines

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Summary

The pattern of subtypes of the nucleosomal histories and of historic Hl was investigated in human cells from adult and fetal lung and liver, from carcinoma tissues and from carcinoma-derived cell lines, with the object of comparing these patterns, and their relationship to cell growth rate, with those in cells of other species. The subtype pattern of the nucleosomal histories H2A and H3 shows a correlation with replication rate. In adult tissues, subtype H3-3 predominates over H3-2 and H3-1, and the subtype H2A-1 and H2A-2 are approximately equally abundant. In fetal tissues, lung carcinoma and cultured carcinoma-derived cell lines, the subtype H3-1 is predominant and H2A-1 is more abundant than H2A-2. The subtype pattern of H 1 also differs between normal and carcinoma cells, among different tissues, and in different cell lines derived from the same type of carcinoma. In particular, the relative level of H1° differs in several cell lines showing relatively high rates of replication, and in some cases represents more than 25% of the total H1, similar to the level in slowly replicating normal adult liver and lung tissues. The relative level of H1° does not therefore appear to be correlated in a simple manner with cell growth rate in these human cells.

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Abbreviations

PhMeS02 :

phenylmethylsulfonyl fluoride

SDS:

sodium dodecyl sulphate

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Authors and Affiliations

  1. Istituto di Chimica Biologica, University degli Studi di Pisa, Scuola Medica, Via Roma 55, 56100, Pisa, Italy

    Franco Gabriellia

  2. The Wistar Institute of Anatomy and Biology, 36th Street at Spruce, 19104, Philadelphia, PA, USA

    David P. Aden

  3. Institut Ludwig de Recherches sur le Cancer, CH-1066, Epalinges/Lausanne, Switzerland

    Stefan C. Carrele

  4. Department of Clinical Pharmacology, Karolinska Institute, S-141, 86 Huddinge, Sweden

    Christer von Bahrd & Anders Raned

  5. Clinica Chirurgica Generale, University degli Studi di Pisa, Via Roma 67, 56100, Pisa, Italy

    Carlo A. Angelettie

  6. Institut Suisse de Recherches Expérimentales sur le Cancer, CH-1066, Epalingesl Lausanne, Switzerland

    Ronald Hancockf

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  1. Franco Gabriellia
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  2. David P. Aden
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  3. Stefan C. Carrele
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  4. Christer von Bahrd
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  5. Anders Raned
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  6. Carlo A. Angelettie
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  7. Ronald Hancockf
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Gabriellia, F., Aden, D.P., Carrele, S.C. et al. Histone complements of human tissues, carcinomas, and carcinoma-derived cell lines. Mol Cell Biochem 65, 57–66 (1984). https://doi.org/10.1007/BF00226019

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  • DOI: https://doi.org/10.1007/BF00226019

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