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Random chloroplast segregation and frequent mtDNA rearrangements in fertile somatic hybrids between Nicotiana tabacum L. and N. glutinosa L.

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Abstract

Patterns of organelle inheritance were examined among fertile somatic hybrids between allotetraploid Nicotiana tabacum L. (2n=4x=48) and a diploid wild relative N. glutinosa L. (2n=2x=24). Seventy somatic hybrids resistant to methotrexate and kanamycin were recovered following fusion of leaf mesophyll protoplasts of transgenic methotrexate-resistant N. tabacum and kanamycin-resistant N. glutinosa. Evidence for hybridization of nuclear genomes was obtained by analysis of glutamate oxaloacetate transaminase and peroxidase isoenzymes and by restriction fragment length polymorphism (RFLP) analysis using a heterologous nuclear ribosomal DNA probe. Analysis of chloroplast genomes in a population of 41 hybrids revealed a random segregation of chloroplasts since 25 possessed N. glutinosa chloroplasts and 16 possessed N. tabacum chloroplasts. This contrasts with the markedly non-random segregation of plastids in N. tabacum (+)N. rustica and N. tabacum (+) N. debneyi somatic hybrids which we described previously and which were recovered using the same conditions for fusion and selection. The organization of the mitochondrial DNA (mtDNA) in 40 individuals was examined by RFLP analysis with a heterologous cytochrome B gene. Thirty-eight somatic hybrids possessed mitochondrial genomes which were rearranged with respect to the parental genomes, two carried mtDNA similar to N. tabacum, while none had mtDNA identical to N. glutinosa. The somatic hybrids were self-fertile and fertile in backcrosses with the tobacco parent.

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Author notes

  1. P. A. Donaldson & E. E. Bevis

    Present address: Plant Research Centre, Agriculture Canada, K1A OC6, Ottawa, Ontario, Canada

Authors and Affiliations

  1. Imperial Tobacco Ltd., P.O. Box 6500, H3C 3L6, Montréal, Quebec, Canada

    P. A. Donaldson & E. E. Bevis

  2. Plant Research Centre, Agriculture Canada, K1A OC6, Ottawa, Ontario, Canada

    R. S. Pandeya & S. C. Gleddie

Authors
  1. P. A. Donaldson
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  2. E. E. Bevis
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  3. R. S. Pandeya
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  4. S. C. Gleddie
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Additional information

Communicated by P. Maliga

Contribution No. 1487 Plant Research Centre

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Donaldson, P.A., Bevis, E.E., Pandeya, R.S. et al. Random chloroplast segregation and frequent mtDNA rearrangements in fertile somatic hybrids between Nicotiana tabacum L. and N. glutinosa L.. Theoret. Appl. Genetics 87, 900–908 (1994). https://doi.org/10.1007/BF00225783

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  • DOI: https://doi.org/10.1007/BF00225783

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