Skip to main content
SpringerLink
Log in

Construction of a high-resolution genetic map and YAC-contigs in the tomato Tm-2a region

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

With the ultimate goal of cloning the Tobacco Mosaic Virus (TMV) resistance gene Tm-2a from tomato by means of positional cloning, a high-resolution map of a 4.3-cM region surrounding the Tm-2a gene has been constructed. In total, 13 RFLP and RAPD markers were mapped in close proximity to Tm-2a using 2112 individuals from an intraspecific Lycopersicon peruvianum backcross. The closest flanking markers were separated from Tm-2a by 0.05 cM on each side. Only one marker, the cDNA clone R12, co-segregated with Tm-2a. In order to physically cover the Tm-2a region, R12 and the flanking DNA marker TG207 were used to select homologous YAC clones. To-date, two YAC-contigs spanning approximately 340 kb and 360 kb have been constructed. The data obtained from these experiments indicate that recombination around the centromere of chromosome 9 is extremely suppressed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alpert KB, Grandillo S, Tanksley SD (1995) fw2.2: a major QTL controlling fruit weight is common to both red -and green-fruited tomato species. Theor Appl Genet 91:994–1000

    Google Scholar 

  • Alexander LJ (1971) Host-pathogen dynamics of tobacco mosaic virus on tomato. Phytopathology 61:611–617

    Google Scholar 

  • Balint-Kurti PJ, Dixon MS, Jones DA, Norcott KA, Jones JDG. (1994) RFLP linkage analysis of the Cf-4 and Cf-9 genes for resistance to Cladosporium fulvum in tomato. Theor Appl Genet 88:691–700

    Google Scholar 

  • Bent AF, Kunkel BN, Dahlbeck D, Brown KL, Schmidt R, Giraudat J, Leung J, Staskawicz BJ (1994) Rps2 of Arabldopsis thaliana: a leucine-rich repeat class of plant disease resistance genes. Science 265:1856–1860

    Google Scholar 

  • Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898

    Google Scholar 

  • Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet 32:314–331

    CAS  PubMed  Google Scholar 

  • Brommonschenkel SH, Cho JJ, Custer D, Tanksley SD (1996) Genetics and high-resolution RFLP linkage mapping of tomato spotted wilt tospovirus (TSWV) resistance derived from Lycopersicon peruvianum. Mol Plant-Microbe Interact (in press)

  • Broun P, Tanksley SD (1996) Characterization and genetic mapping of simple repeat sequences in the tomato genome. Mol Gen Genet 250:39–49

    Google Scholar 

  • Chunwongse J, Bunn TB, Crossman C, Jiang J, Tanksley SD (1994) Chromosomal localization and molecular-marker tagging of the powdery mildew resistance gene (Lv) in tomato. Theor Appl Genet 89:76–79

    Google Scholar 

  • Cirulli M, Alexander LJ (1969) Influence of temperature and strain of tobacco mosaic virus on resistance in a tomato breeding line derived from Lycopersicon peruvianum. Phytopathology 59: 1287–1297

    Google Scholar 

  • Citovsky V, McLean BG, Zupan JR, Zambryski P (1993) Phosphorylation of tobacco mosaic virus cell-to-cell movement protein by a developmentally regulated plant cell wall-associated protein kinase. Genes Dev 7:904–910

    Google Scholar 

  • Clayberg CD (1961) Additional data on the Tm2a-nv linkage. Tomato Genet Coop Rep 11:10

    Google Scholar 

  • Collins FS (1995) Positional cloning moves from perditional to traditional. Nature Genetics 9:347–350

    Google Scholar 

  • Dixon MS, Jones DA, Hatzixanthis K, Ganal MW, Tanksley SD, Jones JDG (1995) High-resolution mapping of the physical location of the tomato Cf-2 gene. Mol Plant-Microbe Interact 8:200–206

    Google Scholar 

  • Frary A, Presting GG, Tanksley SD (1996) Molecular mapping of the centromeres of tomato chromosomes 7 and 9. Mol Gen Genet 250:295–304

    Google Scholar 

  • Fulton TM, Chunwongse J, Tanksley SD (1995) Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol Biol Rep 13:207–209

    CAS  Google Scholar 

  • Ganal MW, Tanksley SD (1996) Recombination around the Tm-2a and Mi resistance genes in different crosses of Lycopersicon peruvianum. Theor Appl Genet 92:101–108

    Article  CAS  Google Scholar 

  • Ganal MW, Young ND, Tanksley SD (1989) Pulsed-field gel electrophoresis and physical mapping of large DNA fragments in the Tm-2a region of chromosome 9 in tomato. Mol Gen Genet 215:395–400

    Google Scholar 

  • Giovannoni JJ, Noensie EN, Ruezinsky DM Lu X, Tracy SL, Ganal MW, Martin GB, Pillen K, Alpert kb, Tanksley SD (1995) Molecular genetic analysis of the ripening-inhibitor and non-ripening loci of tomato: a first step in genetic map-based cloning of fruit-ripening genes. Mol Gen Genet 248:195–206

    Google Scholar 

  • Hall TJ (1980) Resistance at the Tm-2 locus in the tomato to tomato mosaic virus. Euphytica 29:189–197

    Google Scholar 

  • Hannappel U, Balzer HJ, Ganal MW (1995) Direct isolation of cDNA sequences from specific chromosomal regions of the tomato genome by the differential display technique. Mol Gen Genet 249:19–24

    Google Scholar 

  • Jones DA, Thomas CM, Hammond-Kosack KE, Balint-Kurti PJ, Jones JDG (1994) Isolation of the tomato Cf-9 gene for resistance to Cladosporium fulvum by transposon tagging. Science 266:789–793

    Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Khush GS, Rick CM (1968) Cytogenetic analysis of the tomato genome by means of induced deficiencies. Chromosoma 23:452–484

    Google Scholar 

  • Khush GS, Rick CM, Robinson RW (1964) Genetic activity in a heterochromatic chromosome segment of the tomato. Science 145:1432–1434

    Google Scholar 

  • Liang P, Pardee AB (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967–971

    CAS  PubMed  Google Scholar 

  • Martin GB, Ganal MW, Tanksley SD (1992) Construction of a yeast artificial chromosome library of tomato and identification of cloned segments linked to two disease-resistance loci. Mol Gen Genet 233:25–32

    Google Scholar 

  • Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432–1436

    CAS  PubMed  Google Scholar 

  • Meshi T, Motoyoshi F, Adachi A, Watanabe Y, Takamatsu N, Okada Y (1988) Two concomitant base substitutions in the putative replicase genes of tobacco mosaic virus confer the ability to overcome the effects of a tomato resistance gene, Tm-1. EMBO J 7:1575–1581

    Google Scholar 

  • Meshi T, Motoyoshi F, Maeda T, Yoshiwoka S, Watanabe Y, Okada Y (1989) Mutations in the tobacco mosaic virus 30-kDa protein gene overcome Tm-2 resistance in tomato. Plant Cell 1:515–522

    Google Scholar 

  • Messeguer R, Ganal MW, DeVicente MC Young ND, Bolkan H, Tanksley SD (1991) High-resolution RFLP map around the root knot nematode resistance gene Mi in tomato. Theor Appl Genet 82:529–536

    Google Scholar 

  • Ochman H., Gerber AS, Hartl DL (1988) Genetic applications of an inverse polymerase chain reaction. Genetics 120:621–624

    CAS  PubMed  Google Scholar 

  • Paterson AH, Lander ES, Hewitt JD, Paterson S, Lincoln SE, Tanksley SD (1988) Resolution of quantitative traits into Mendelian factors, using a complete linkage map of restriction fragment length polymorphism. Nature 335:721–726

    Article  CAS  PubMed  Google Scholar 

  • Pillen K, Alpert KB, Giovannoni JJ, Ganal MW, Tanksley SD (1996) Rapid and reliable screening of a tomato YAC library exclusively based on PCR. Plant Mol Biol Rep 14:58–67

    Google Scholar 

  • Rafalski JA. Tingey SV (1993) Genetic diagnostics in plant breeding: RAPDs, microsatellites and machines. Trends Genet 9:275–280

    Article  CAS  PubMed  Google Scholar 

  • Rick CM (1969) Controlled introgression of chromosomes of Solanum pennellii into Lycopersicon esculentum: Segregation and recombination. Genetics 128:1–5

    Google Scholar 

  • Schroeder WT, Providenti R, Robinson RW (1967) Incubation temperature and virus strains important in evaluating tomato genotypes for tobacco mosaic virus reactions. Tomato Genet Coop Rep 17:47–48

    Google Scholar 

  • Schumacher K, Ganal M, Theres K (1995) Genetic and physical mapping of the lateral suppressor (ls) locus in tomato. Mol Gen Genet 246:761–766

    Google Scholar 

  • Segal G, Sarfatti M, Schaffer MA, Ori N, Zamir D, Fluhr R (1992) Correlation of genetic and physical structure in the region surrounding the I-2 Fusarium oxysporum resistance locus in tomato. Mol Gen Genet 231:179–185

    Google Scholar 

  • Siegel A, Wildman SG (1954) Some natural relationships among strains of tobacco mosaic virus. Phytopathology 44:277–282

    Google Scholar 

  • Suiter K, Wendel J, Case J (1983) LINKAGE-1: A pascal computer program for detection and analysis of genetic linkage. J Hered 74:203–204

    Google Scholar 

  • Tanksley SD, Ganal MW, Prince JP, DeVicente MC, Bonierbale MW, Broun P, Fulton TM, Giovannoni JJ, Grandillo S, Martin GB, Messeguer R, Miller JC, Miller L, Paterson AH, Pineda O, Röder MS, Wing RA, Wu W, Young ND (1992) High-density molecular linkage maps of the tomato and potato genomes. Genetics 132:9

    Google Scholar 

  • Tanksley SD, Ganal MW, Martin GB (1995) Chromosome landing: a paradigm for map-based gene cloning in plant species with large genomes. Trends Genet 11:63–68

    Google Scholar 

  • Weber H, Schultze S, Pfitzner AJP (1993) Two amino-acid substitutions in the tomato mosaic virus 30-kDa movement protein confer the ability to overcome the Tm-2 2 resistance gene in the tomato. J Virol 67:6432–6438

    Google Scholar 

  • Weissenbach J, Gyapay G, Dib C, Vignal A, Morisette J, Millasseau P, Vaysseix G, Lathrop M (1992) A second-generation linkage map of the human genome. Nature 359:794–801

    Google Scholar 

  • Whitham S, Dinesh-Kumar SP, Choi D, Hehl R, Corr C, Baker B (1994) The product of the tobacco mosaic virus resistance gene N: similarity to Toll and the interleukin-1 receptor. Cell 78:1101–1115

    Google Scholar 

  • Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segments retained around the Tm-2a locus of tomato during backcross breeding. Theor Appl Genet 77:353–359

    Google Scholar 

  • Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to the Tm-2a gene in tomato. Genetics 120:579–586

    Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Fritjers A, Pot J, Peleman J, Kuiper M, Zabeau M (1996) AFLP: a new concept for DNA fingerprinting. Nucleic Acids Res (in press)

  • Zhang HB, Martin GB, Tanksley SD, Wing RA (1994) Map-based cloning in crop plants: tomato as a model system II. Isolation and characterization of a set of overlapping yeast artificial chromosomes encompassing the jointless locus. Mol Gen Genet 244:613–621

    Google Scholar 

Download references

Author information

Author notes

  1. K. Pillen

    Present address: Institute of Agronomy, University of Bonn, Katzenburgweg 5, 53115, Bonn, Germany

Authors and Affiliations

  1. Department of Plant Breeding and Biometry, 252 Emerson Hall, Cornell University, 14853, Ithaca, NY, USA

    K. Pillen & S. D. Tanksley

  2. Institute of Plant Genetics and Crop Plant Research, Corrensstr. 3, 06466, Gatersleben, Germany

    M. W. Ganal

Authors
  1. K. Pillen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. W. Ganal
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. D. Tanksley
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pillen, K., Ganal, M.W. & Tanksley, S.D. Construction of a high-resolution genetic map and YAC-contigs in the tomato Tm-2a region. Theoret. Appl. Genetics 93, 228–233 (1996). https://doi.org/10.1007/BF00225750

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00225750

Key words

Search

Navigation