Summary
Busulphan and Endoxan® inhibit the normal course of ovarian follicular growth. Older secondary as well as all antral follicles perish within a very short time.
Growing follicles, which at the beginning of the experiment exhibited only one layer, remained intact and single-layered during the entire duration of the experiment. The oocytes, however, continue growing, and the cytoplasmic structures, which are characteristic of older growing follicles, develop in them as well as in the follicle cells. Even a theca formation develops.
In some of the “growing” follicles which have remained single-layered, after 10 days of Busulphan administration, some liquor folliculi is produced and accumulates in a fissure-shaped “antrum” between the zona pellucida and the follicular epithelium.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Albertini, D.F., Anderson, E.: The appearance and structure of intercellular connections during the ontogeny of the rabbit ovarian follicle with particular reference to gap junctions. J. Cell Biol. 63, 234–250 (1974)
Anderson, E., Albertini, D.F.: Gap junctions between the oocyte and companion follicle cells in the mammalian ovary. J. Cell Biol. 71, 680–686 (1976)
Björkman, N.: A study of the ultrastructure of the granulosa cells of the rat ovary. Acta anat. (Basel) 51, 125–147 (1962)
Burkl, W., Kellner, G.: Der Einfluß von Myleran auf das Wachstum der Follikel im Rattenovar. Anat. Anz. 100, 322–325 (1953/54)
Burkl, W., Schiechl, H.: Lichtund elektronenmikroskopische Untersuchungen an den Scheinkernen fragmentierter Eizellen im Rattenovar. Z. mikr.-anat. Forsch. 90, 273–283 (1976)
Burkl, W., Thiel-Bartosch, E.: Elektronenmikroskopische Untersuchungen über die Granulosa atresierender Tertiärfollikel bei der Ratte. Arch. Gynäk. 204, 238–250 (1967)
Chiquoine, A.D.: The development of the zona pellucida of the mammalian ovum. Amer. J. Anat. 106, 149–170 (1960)
Forsberg, J.-G., Olivecrona, H.: The effect of prenatally administered Busulphan on rat gonads. Biol. Neonat. (Basel) 10, 180–192 (1966)
Hemsworth, B.N., Jackson H.: Effect of Busulphan on the developing ovary in the rat. J. Reprod. Fertil. 6, 229–233 (1963)
Knigge, K.M., Leathem, J.H.: Growth and atresia of follicles in the ovary of the hamster. Anat. Rec. 124, 679–703 (1956)
Mandl, A.M., Zuckerman, S.: The growth of the oocyte and follicle in the adult rat. J. Endocr. 8, 126–132 (1952)
Merker, H.-J.: Elektronenmikroskopische Untersuchungen über die Bildung der Zona pellucida in den Follikeln des Kaninchenovars. Z. Zellforsch. 54, 677–688 (1961)
Motta, P., Svetanca, T., Nesci, E.: Etude ultrastructurale et histochimique des rapports entre les cellules folliculaires et l'ovocyte pendant le développement du follicule ovarien chez les mammifères. Acta anat. (Basel) 80, 537–562 (1971)
Nørrevang, A.: Electron microscopic morphology of oogenesis. Int. Rev. Cytol. 23, 114–176 (1968)
Odor, D.L.: Electron microscopic studies on ovarian oocytes and unfertilized tubal ova in the rat. J. biophys. biochem. Cytol. 7, 567–574 (1960)
Odor, D.L.: The ultrastructure of unilaminar follicles of the hamster ovary. Amer. J. Anat. 116, 493–522 (1965)
Pedersen, T.: Determination of follicle growth rate in the ovary of the immature mouse. J. Reprod. Fertil. 21, 81–93 (1970a)
Pedersen, T.: Follicle kinetics in the ovary of the cyclic mouse. Acta endocr. (Kbh.) 64, 304–323 (1970b)
Peters, H., Levy, E.: Cell dynamics of the ovarian cycle. J. Reprod. Fertil. 11, 227–236 (1966)
Rateau, J.-G.: Etude de la différenciation de l'ovaire du rat après traitement par le Busulfan. C.R. Soc. Biol. (Paris) 168, 1163–1167 (1974a)
Rateau, J.-G.: Etude expérimentale de la maturation folliculaire et glandulaire des ovaires de rattes traitées par le Busulfan. C.R. Soc. Biol. (Paris) 168, 1168–1172 (1974b)
Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–229 (1963)
Schaar, H.: Funktionelle Morphologie der Theca interna im Bläschenfollikel des menchlichen Ovars. Acta anat. (Basel) 94, 283–298 (1976)
Slater, D.W., Dornfield, E.J.: Quantitative aspects of growth and oocyte production in the early prepubertal rat ovary. Amer. J. Anat. 76, 253–267 (1945)
Weakley, B.S.: Electron microscopy of the oocyte and granulosa cells in the developing ovarian follicles of the golden hamster (Mesocricetus auratus). J. Anat. (Lond.) 100, 503–534 (1966)
Weakley, B.S.: Comparison of cytoplasmic lamellae and membranous elements in the oocytes of five mammalian species. Z. Zellforsch. 85, 109–123 (1968)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Burkl, W., Schiechl, H. The growth of follicles in the rat ovary under the influence of Busulphan and Endoxan® . Cell Tissue Res. 186, 351–359 (1978). https://doi.org/10.1007/BF00225543
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225543