Summary
The distribution and abundance of simple repetitive sequences complementary to the synthetic oligonucleotides (GACA)4, (GATA)4, (GTG)5 and (CA)8 in the genomes of several cultivars of Beta vulgaris and in the wild beet B. vulgaris ssp. maritima were investigated. Hybridization experiments revealed that all four motifs were present, though at different abundances, in the genomes of all of the investigated beet cultivars. Considerable intraspecific variation of the resulting DNA fingerprints was observed. The extent of polymorphism depends on the oligonucleotide probe. The most informative banding patterns were obtained with the (GATA)4 probe hybridized to HinfI-, HaeIII-, or RsaI-restricted DNA, respectively. DNA fingerprinting with (GATA)4 allowed a clear differentiation of double-haploid breeding lines (DH lines). We demonstrated that the application of oligonucleotide probes for DNA fingerprinting is a sensitive tool for genome diagnosis in cultivated beet.
Similar content being viewed by others
References
Ali S, Müller CR, Epplen JT (1986) DNA fingerprinting by oligonucleotide probes specific for simple repeats. Hum Genet 74:239–243
Barocka KH (1985) Zucker- und Futterrüben. In: Hoffmann W, Mudra A, Plarre W (eds) Lehrbuch der Züchtung landwirtschaftlicher Kulturpflanzen, vol 2: Spezieller Teil. Parey, Berlin, Hamburg, pp 245–287
Bossoutrot D, Hosemans D (1985) Gynogenesis in Beta vulgaris L.: from in vitro culture of unpollinated ovules to the production of double-haploid plants in soil. Plant Cell Rep 4:300–303
Condit R, Hubbell SP (1991) Abundance and DNA sequence of two-base repeat regions in tropical tree genomes. Genome 34:66–71
Epplen JT, McCarrey JR, Sutou S, Ohno S (1982) Base sequence of a cloned snake W-chromosome DNA fragment and identification of a male-specific putative mRNA in the mouse. Proc Natl Acad Sci USA 79:3798–3802
Nagamine T, Todd GA, McCann KP, Newbury HJ, Ford-Lloyd BV (1989) Use of restriction fragment length polymorphism to fingerprint beets at the genotype and species levels. Theor Appl Genet 78:847–851
Opstelten RJG, Clement JMF, Wanka F (1989) Direct repeats at nuclear-matrix associated DNA regions and their putative control function in the replicating eukaryotic genome. Chromosoma 98:422–427
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Schmidt T, Metzlaff M (1991) Cloning and characterization of a Beta vulgaris satellite DNA family. Gene 101:247–250
Schmidt T, Junghans H, Metzlaff M (1990) Construction of Beta procumbens-specific DNA probes and their application for the screening of B.vulgaris x B. Procumbens (2n = 19) addition lines. Theor Appl Genet 79:177–181
Schmidt T, Jung C, Metzlaff M (1991) Distribution and evolution of satellite DNA families in the genus Beta. Theor Appl Genet 82:793–799
Schweizer G, Ganal M, Ninnemann H, Hemleben V (1988) Species-specific DNA sequences for identification of somatic hybrids between Lycopersicon esculentum and Solanum acaule. Theor Appl Genet 75:679–684
Studer R, Kammerbauer C, Zischler H, Hinkkanen A (1991) Highly unstable (GATA)n-containing sequences of the mouse during the cloning process. Electrophoresis 12:153–158
Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding: new tools for an old science. Biotechnology 7:257–264
Thein SL, Wallace RB (1986) The use of synthetic oligonucleotides as specific hybridization probes in the diagnosis of genetic disorders. In: Davies KE (ed) Human genetic diseases — a practical approach. IRL Press, Oxford, pp 33–50
Weising K, Beyermann B, Ramser J, Kahl G (1991a) Plant DNA fingerprinting with radioactive and digoxygenated oligonucleotide probes complementary to simple repetitive DNA sequences. Electrophoresis 12:159–169
Weising K, Ramser J, Kaemmer D, Kahl G, Epplen JT (1991b) Oligonucleotide fingerprinting in plants and fungi. In: Burke T, Dolf G, Jeffreys AJ, Wolff R (eds) DNA Fingerprinting: approaches and applications. Birkhäuser, Basel, pp 312–329
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Schmidt, T., Boblenz, K., Metzlaff, M. et al. DNA fingerprinting in sugar beet (Beta vulgaris) — identification of double-haploid breeding lines. Theoret. Appl. Genetics 85, 653–657 (1993). https://doi.org/10.1007/BF00225001
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00225001