Summary
A strategy for the production of non-inhibitory polyclonal antisera for the immobilization of glycoenzymes is described. Insoluble complexes of invertase prepared using antiinvertase glycosyl antisera were more resistant to heat and urea induced denaturation than those prepared using antiinvertase antisera from which the glycosyl specific antibodies were removed. Unfractionated antiinvertase antibodies however gave most stable immunocomplexes with invertase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Refernces
Bernfeld, P. (1955) methods Enzymol., (eds. Colowiek, S.P. and Kaplan, N.O.) Academic Press, New York, vol., 1, p. 149.
Chotia, C. and Janin, J. (1975) Nature, 256, 705–708.
Davies, D.R., Sheriff, S. and Paldan, E.A. (1988) J. Biol. Chem., 263, 10541–10544.
de Alwis, U. and Wilson, G.S. (1989) Talanta, 36, 249–253.
Dubois, M. K., Gilles, A., Hamilton, J.K., Rebers, P.A. and Smith, F. (1956) Anal Biochem., 28, 350.
Feinstein, R.N., Jaroslow, B.N., Howard, J.B. and Faulhaber, J.T. (1971) J. Immunol. 106, 1316–1322.
Fusek, M., Turkova, J., Stovickova, J. and Franck, F. (1988) Biotechnol. Lett., 10, 85–90.
Guisan, J.M., Bastida, A., Cuesta, C., Fernandez-Lafuente, R. and Rosell, M. (1991) Biotechnol. Bioeng., 38, 1144–1152.
Habeeb, A.F.S.A. (1966) Anal. Biochem., 14, 328–336.
Hsiao, H. and Royer, G.P. (1979) Arch. Biochem. Biophys., 20, 33–38.
Husain, Q., Iqbal, J. and Saleemuddin, M. (1985) Biotechnol. Bioeng., 27, 1102–1107.
Iqbal, J. and Saleemuddin, M. (1983) Biotechnol. Bioeng., 25, 3191–3195.
Jafri, F., Husain, S. and Saleemuddin, M. (1993) Biotechnol. Appl. Biochem., 18, 401–408.
Lis, H. and Sharon, N. (1993) Eur. J. Biochem., 218, 1–27.
Porath, J., Axen, R. and Ernback, S. (1967) Nature, 215, 1491.
Saleemuddin, M. and Husain, Q. (1991) Enzyme Microb. Technol., 13, 290–295.
Shami, E.Y., Rothstein, A. and Ramjeesingh, M. (1989) Trends Biotechnol, 7, 186–190.
Shami, E.Y., Ramjeesingh, M., Rothstein, A. and Zywulko, M. (1991) Enzyme Microb. Technol., 13, 424–429.
Soloman, B., Koppel, R., Pines, G. and Katenalski-katzir, E. (1986) Biotechnol. Bioeng., 28, 1213–1221.
Stovickova, J., Franck, F. and Turkova, J. (1991) Biocatalysis, 5, 121–130.
Ulbrich-Hoffman, R., Golbik, R. and Dameran, W. (1993) in Stability and Stabilization of enzymes (W.J.J. Van der Tweei, A. Harder and R.M. Buitelar editors) Elsevier Science Publishers B.V. pp 497–504.
Woodward, J. and Wiseman, A. (1978) Biochim. Biophys. Acta, 527, 8–16.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jafri, F., Husain, S. & Saleemuddin, M. Stabilization of yeast invertase by insoluble immunocomplex formation using unfractionated glycosyl-specific and peptide specific polyclonal antiinvertase antibodies. Biotechnol Tech 9, 117–122 (1995). https://doi.org/10.1007/BF00224409
Issue Date:
DOI: https://doi.org/10.1007/BF00224409