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Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition

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Summary

Mutants of Arabidopsis thaliana were identified by screening pedigreed M3 seed collections from EMS-treated plants for changes in fatty acid (FA) composition. The FA phenotypes of the most dramatic mutants are as follows: G30 and 1E5 (allelic) lack linolenic acid (18∶3) and are elevated in linoleic acid (18∶2); 4A5 is deficient in 18∶2 and 18∶3 and fourfold increased in oleic acid (18∶1); 9A1 lacks all FAs > C18 and is twofold increased in 18∶1; 1A9 is twofold increased in palmitic acid (16∶0) and decreased by one-half in 18∶1; 2A11 is two-to threefold increased in stearic acid (18∶0) and decreased by one-half in 18∶1. Based on segregation of F2 selfed plants derived from crosses to wild type, all of these phenotypes are the result of single gene mutations.

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Authors and Affiliations

  1. DNA Plant Technology, 6701 San Pablo Ave, 94608, Oakland, CA, USA

    D. W. James Jr. & H. K. Dooner

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  1. D. W. James Jr.
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  2. H. K. Dooner
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Communicated by P. Maliga

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James, D.W., Dooner, H.K. Isolation of EMS-induced mutants in Arabidopsis altered in seed fatty acid composition. Theoret. Appl. Genetics 80, 241–245 (1990). https://doi.org/10.1007/BF00224393

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  • DOI: https://doi.org/10.1007/BF00224393

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