Summary
Total seed storage protein of the cultivated chickpea, C. arietinum L., and eight other wild annual Cicer species (all 2n = 16) was separated and compared by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The seed-protein profile was a conservative and species-specific trait. Relative interspecific similarities of protein patterns were estimated using Jaccard's similarity index, and a cluster analysis was performed. The resultant dendrogram generally agreed with the limited data already available on interspecific relationships in Cicer based on morphological characteristics, crossability, genome pairing in hybrids, karyotypes and isozyme analysis. The difference between the profiles of C. judaicum and C. pinnatifidum supported the idea that they are indeed two separate species. The closest relative of C. arietinum was C. reticulatum, followed by C. echinospermum and other species, while C. cuneatum was the farthest relative. In general, C. cuneatum was also genetically the farthest removed from any other species. The suggestion that C. reticulatum is the wild progenitor of the cultivated chickpea was therefore further supported.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ahmad F (1988) Interspecific hybridization and genetic relationships among the annual Cicer L. species. Ph.D thesis, University of Saskatchewan, Saskatoon, Canada.
Ahmad F, Slinkard AE, Scoles GJ (1987a) Karyotypic analysis of the annual Cicer L. species. Genet Soc Can Bull 18 [Suppl 1]: 130.
Ahmad F, Slinkard AE, Scoles GJ (1987b) The cytogenetic relationship between Cicer judaicum Boiss. and Cicer chorassanicum (Bge.) M. Pop. Genome 29:883–886.
Ahmad F, Gaur PM, Slinkard AE (1988a) Isozyme based phylogeny of the annual Cicer L. species. Genome 30 [Suppl 1]:404.
Ahmad F, Slinkard AE, Scoles GJ (1988b) Investigations into the barrier (s) to interspecific hybridization between Cicer arietinum L. and eight other annual Cicer species. Plant Breed 100:193–198.
Boulter D (1981) Proteins of legumes. In: Raven PH (ed) Advances in legume systematics, part 2. Royal Botanic Gardens, Kew, UK, pp 501–512.
DeJong WW, Zweers A, Cohen LH (1978) Influence of single amino acid substitutions or deletions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes. Biochem Biophys Res Commun 82:532–539.
Duke EJ, Glassman E (1968) Evolution of xanthine dehydrogenase in Drosophila. Genetics 58:101–112.
Jackson P, Milton JM, Boulter D (1967) Fingerprinting patterns of the globulin fraction obtained from seeds of various species of Fabaceae. New Phytol 66:47–56.
Kimber G (1984) Evolutionary relationships and their influence on plant breeding. In: Gustafson JP (ed) Gene manipulation in plant improvement. Plenum Press, New York, pp 281–300.
Ladizinsky G (1975) Seed protein electrophoresis of the wild and cultivated species of the section Faba of Vicia. Euphytica 24:785–788.
Ladizinsky G (1979) Species relationships in the genus Lens as indicated by seed protein electrophoresis. Bot Gaz 140:449–451.
Ladizinsky G. Adler A (1975) The origin of chickpea as indicated by seed protein electrophoresis. Isr J Bot 24:183–189.
Ladizinsky G, Adler A (1976a) Genetic relationships among the annual species of Cicer L. Theor Appl Genet 48:197–203.
Ladizinsky G, Adler A (1976b) The origin of chickpea, Cicer arietinum L. Euphytica 25:211–217.
Ladizinsky G, Hymowitz T (1979) Seed protein electrophoresis in taxonomic and evolutionary studies. Theor Appl Genet 54:145–151.
Laemmli VK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.
Margoliash E, Fitch WM (1968) Evolutionary variability of cytochrome “c” primary structures. Ann NY Acad Sci 151:359–381.
Panda RC, Kumar OA, Rao KGR (1986) The use of seed protein electrophoresis in the study of phylogenetic relationships in chili pepper (Capsicum L.). Theor Appl Genet 72:665–670.
Pundir RPS, van der Maesen LJG (1983) Interspecific hybridization in Cicer. Int Chickpea Newsl 8:4–5.
Sammour RH (1989) Electrophoresis of the seed proteins of Vicia faba L. and its immediate progenitors — A reappraisal. Plant Breed 104:196–201.
Siddiq EA, Nerkar YS, Mehta SL (1972) Intra and inter-specific variation in soluble proteins of Oryza sativa L. Theor Appl Genet 42:351–356.
Sneath PHA, Sokal RR (1973) Numerical taxonomy. WH Freeman, San-Francisco, pp 100–308.
Vairinhos F, Murray DR (1982) Variations in the size of both large and small disulphide-linked subunits of legumin in representatives of Viceae and Cicer. Z Pflanzenphysiol 107:5–32.
Vairinhos F, Murray DR (1983) The seed proteins of chickpea: comparative studies of Cicer arietinum, C. reticulatum and C. echinospermum (Leguminosae). Plant Syst Evol 142:11–22.
van der Maesen LJG (1972) Cicer L.: a monograph of the genus, with special reference to the chickpea (Cicer arietinum L.), its ecology and cultivation. Ph.D thesis. Agricultural University of Wageningen Agricultural college information bulletin Wageningen 72–10.
van der Maesen LJG (1987) Origin, history and taxonomy of chickpea. In: Saxena MC, Singh KB (eds) The chickpea. CAB Int Publ UK, pp 11–34.
van der Maesen LJG, Pundir RPS (1984) Availability and use of wild Cicer germ plasm. Plant Genet Res Newsl 57:19–24.
Wolf FG (1980) Investigations on the relations within the family Papilionaceae on the basis of electrophoretic banding patterns. Theor Appl Genet 57:225–232.
Author information
Authors and Affiliations
Additional information
Communicated by G. S. Khush
Rights and permissions
About this article
Cite this article
Ahmad, F., Slinkard, A.E. Genetic relationships in the genus Cicer L. as revealed by polyacrylamide gel electrophoresis of seed storage proteins. Theoret. Appl. Genetics 84, 688–692 (1992). https://doi.org/10.1007/BF00224169
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224169