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Insect-resistant plants with improved horticultural traits from interspecific potato hybrids grown in vitro

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Summary

Plants were regenerated from petiole calli of interspecific hybrids of Solanum tuberosum x S. berthaultii, an insect-resistant wild species. Callus culture was used to generate genetic changes to overcome the restricted recombination between the two genomes. Two plants out of 58 (3.5%) from calli of hybrid J114-1 showed stable and heritable differences from the hybrid over two cycles of evaluations in the field. Replicated trials were conducted in 1987 and 1988, using two populations of plants propagated by nodal cuttings from the original regenerates maintained in vitro. One regenerate showed insect resistance and increased marketable yield (approximately two fold) in the field. The other had higher levels of phenolic exudate in one of the two types of foliar trichomes associated with the insect resistance mechanism. Some desirable changes were discernible only in sexual progeny of regenerates, not in the regenerates themselves. In a backcross to S. tuberosum, 7 of 14 (50%) regenerates from hybrid F743-4 showed more progeny (up to 15-fold) with improved trichome traits and horticultural characteristics than the original hybrid. The variations were not associated with changes in ploidy. Fifteen plants obtained from these crosses are currently being incorporated into breeding lines. These results suggest that a period of callus culture followed by plant regeneration may aid in the introgression of desirable traits from wild species into crop plants.

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References

  • Austin S, Cassells AC (1983) Variation between plants regenerated from individual calli produced from separated potato stem callus cells. Plant Sci Lett 31:107–114

    Google Scholar 

  • Avé DA, Eannetta NT, Tingey WM (1986) A modified enzymic browning assay for potato glandular trichomes. Am Potato J 63:553–557

    Google Scholar 

  • Avé DA, Gregory P, Tingey WM (1987) Aphid-repellent sesquiterpenes in glandular trichomes of Solanum berthaultii and S. tuberosum. Entomol Exp Appl 44:131–138

    Google Scholar 

  • Bonierbale MW, Plaisted RL, Tanksley SD (1988) RFLP maps based on a common set of clones reveal modes of chromosomal evolution in potato and tomato. Genetics 120:1095–1103

    Google Scholar 

  • Boodley JW, Sheldrake R Jr (1973) Cornell peat-lite mixes for commercial plant growing. NY State Coll Agric Life Sci Coop Ext Bull 43

  • berg I, Glimelius K, Eriksson T (1984) Nuclear DNA content during initiation of callus formation from isolated protoplasts of Solanum tuberosum L. Plant Sci Lett 35:225–230

    Google Scholar 

  • Creissen GP, Karp A (1985) Karyotypic changes in potato plants regenerated from protoplasts. Plant Cell Tiss Org Cult 4:171–182

    Google Scholar 

  • Evans DA, Sharp WR (1983) Single gene mutations in tomato plants regenerated from tissue culture. Science 221:949–951

    Google Scholar 

  • Evans NE, Foulger D, Farrer L, Bright SWJ (1986) Somaclonal variation in explant-derived potato clones over three tuber generations. Euphytica 35:353–361

    Google Scholar 

  • Gibson RW (1971) Glandular hairs providing resistance to aphids in certain potato species. Ann Appl Biol 68:113–119

    Google Scholar 

  • Gibson RW (1979) The geographical distribution, inheritance, and pest-resisting properties of sticky-tipped foliar hairs on plants of Solanum berthaultii and S. tarijense, and the introduction of this character to cultivated potatoes. Potato Res 22:223–236

    Google Scholar 

  • Gibson RW, Turner RH (1977) Insect trapping hairs on potato plants. PANS 22:272–277

    Google Scholar 

  • Gill BS, Kam-Morgan LNW, Shepard JF (1986) Origin of chromosomal and phenotypic variation in potato protoclones. J Hered 77:13–16

    Google Scholar 

  • Gill BS, Kam-Morgan LNW, Shepard JF (1987) Cytogenetic and phenotypic variation in mesophyll cell-derived tetraploid potatoes. J Hered 78:15–20

    Google Scholar 

  • Gregory P, Tingey WM, Avé DA, Bouthyette PY (1986) Potato glandular trichomes, a physiochemical defense mechanism against insects. In: Green MB, Hedin PA (eds) Natural resistance of plants pests. Roles of allelochemicals. ACS Symp Ser 296, Washington/DC

  • Haberlach GT, Cohen BA, Reichert NA, Baer MA, Towill LE, Helgeson JP (1985) Isolation, culture, and regeneration of protoplasts from potato and several related Solanum species. Plant Sci 39:67–74

    Google Scholar 

  • Harborne JB (1973) Phytochemical methods. A guide to modern techniques of plant analysis. Chapman and Hall, London, pp 33–38

    Google Scholar 

  • Hermsen JGT, Ramanna MS, Roest S, Bokelmann GS (1981) Chromosome doubling through adventitious shoot formation on in vitro-cultivated leaf explants from diploid inter-specific potato hybrids. Euphytica 30:239–246

    Google Scholar 

  • Hussey G, Stacey NJ (1981) In vitro propagation of potato (Solanum tuberosum L.). Ann Bot 48:787–796

    Google Scholar 

  • Kalazich JC (1989) Introgression of the trichome characteristics of Solanum berthaultii (Hawkes) into S. tuberosum (L.). PhD Thesis, Cornell University, Ithaca/NY

    Google Scholar 

  • Karp A, Bright SWJ (1985) On the causes and origin of somaclonal variation. Oxford Surv Plant Mol Cell Biol 2:199–234

    Google Scholar 

  • Karp A, Nelson RS, Thomas E, Bright S (1982) Chromosome variation in protoplast derived potato plants. Theor Appl Genet 63:265–272

    Google Scholar 

  • King RB, Pelletier Y, Singh RP, Calhoum LA (1986) 3,4-Di-Oisobutyryl-6-O-caprylsucrose: the major component of a novel sucrose ester complex from the type B glandular trichome of Solanum berthaultii Hawkes (PI 473340). J Chem Soc Chem Commun 14:1078–1079

    Google Scholar 

  • Kovacs MIP, Howes NK, Kerber ER (1986) D-genome suppression of gliadin electrophoretic band 45 in hexaploid wheat and viscoelastic properties of cooked gluten. Ann Wheat Newslett 32:50–51

    Google Scholar 

  • Kowalski SP (1989) Insect resistance in potato: purification and characterization of a polyphenol oxidase from the type A glandular trichomes of Solanum berthaultii Hawkes. PhD Thesis, Cornell University, Ithaca/NY

    Google Scholar 

  • Landsmann J, Uhrig H (1985) Somaclonal variation in Solanum tuberosum detected at the molecular level. Theor Appl Genet 71:500–505

    Google Scholar 

  • Lapitan NLV, Sears RG, Gill BS (1984) Translocations and other karyotypic structural changes in wheat x rye hybrids regenerated from tissue culture. Theor Appl Genet 68:547–554

    Google Scholar 

  • Lapitan NLV, Sears RG, Rayburn AL, Gill BS (1986) Wheat rye translocations: detection of chromosome breakpoints by in situ hybridization with a biotin-labeled DNA probe. J Hered 77:415–419

    Google Scholar 

  • Lapitan NLV, Sears RG, Gill BS (1988) Amplification of repeated DNA sequences in wheat x rye hybrids regenerated from tissue culture. Theor Appl Genet 75:381–388

    Google Scholar 

  • Larkin P, Scowcroft W (1981) Somaclonal variation — a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–214

    Google Scholar 

  • Lazin MB (1980) Screening for heat tolerance and critical photoperiod in the potato (Solanum tuberosum L.). PhD Thesis, Cornell University, Ithaca/NY

    Google Scholar 

  • Lee M, Phillips R (1987) Genomic rearrangements in maize induced by tissue culture. Genome 29:122–128

    Google Scholar 

  • Lentini Z (1989) Somaclonal variation of insect-resistant Solanum berthaultii (Hawkes) x Solanum tuberosum L. hybrids and assessment of earliness for tuber set in vitro. PhD Thesis, Cornell University, Ithaca/NY

    Google Scholar 

  • Mehlenbacher SA, Plaisted RL, Tingey WM (1983) Inheritance of glandular trichomes in crosses with Solanum berthaultii. Am Potato J 60:699–708

    Google Scholar 

  • Mehlenbacher SA, Plaisted RL, Tingey WM (1984) Heritability of trichome density and droplet size in interspecific potato hybrids and relationship to aphid resistance. Crop Sci 24: 320–322

    Google Scholar 

  • Mendoza HA, Haynes FL (1977) Inheritance of tuber initiation in tuber bearing Solanum as influenced by photoperiod. Am Potato J 54:243–252

    Google Scholar 

  • Multani DS, Dhaliwal HS, Singh P, Gill KS (1988) Synthetic amphiploids of wheat as a source of resistance to kernel bunt (Neovossia indica). Plant Breed 101:122–125

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–479

    CAS  Google Scholar 

  • Orton TJ (1980) Haploid barley regenerated from callus cultures of Hordeum vulgare x H. jubatum. J Hered 71:280–282

    Google Scholar 

  • Orton TJ, Steidl RP (1980) Cytogenetic analysis of plants regenerated from colchicine-treated callus cultures of an inter-specific Hordeum hybrid. Theor Appl Genet 57:89–95

    Google Scholar 

  • Pijnacker LP, Ferwerda MA (1987) Karyotype variation in amino-ethylcysteine resistant cell and callus cultures and regenerated plants of a dihaploid potato (Solanum tuberosum). Plant Cell Rep 6:385–388

    Google Scholar 

  • Ryan JD, Gregory P, Tingey WM (1982) Phenolic oxidase activities in glandular trichomes of Solanum berthaultii. Phytochemistry 21:1885–1887

    Google Scholar 

  • Scowcroft WR, Brettell RIS, Ryan SA, Davies PA, Pallota MA (1987) Somaclonal variation and genomic flux. In: Green CE, Somers DA, Hackett WP, Biesboer DD (eds) Plant tissue and cell culture. Plant biology series, Vol. 3. Alan R. Liss, New York, pp 275–286

    Google Scholar 

  • Sibi M, Biglary M, Demarly Y (1984) Increase in the rate of recombinants in tomato (Lycopersicon esculentum L.) after in vitro regeneration. Theor Appl Genet 68:317–321

    Google Scholar 

  • Sree Ramulu K, Dijkhuis P, Roest S (1983) Phenotypic variation and ploidy level of plants regenerated from protoplast of tetraploid potato (Solanum tuberosum L. cv “Bintje”). Theor Appl Genet 65:329–338

    Google Scholar 

  • Sree Ramulu K, Dijkhuis P, Roest S (1984) Genetic instability in protoclones of potato (Solanum tuberosum L. cv “Bintje”): new types of variation after propagation. Theor Appl Genet 68:515–519

    Google Scholar 

  • Sree Ramulu K, Dijkhuis P, Hanisch Ten Cate ChH, De Groot B (1985) Patterns of DNA and chromosome variation during in vitro growth in various genotypes of potato. Plant Sci 41:69–78

    Google Scholar 

  • Swaminathan MS, Howard HW (1953) The cytology and genetics of the potato (Solanum tuberosum) and related species. Bibliogr Genet 16:1–192

    Google Scholar 

  • Tanksley SD, Young ND, Paterson AH, Bonierbale MW (1989) RFLP mapping in plant breeding. New tools for an old science. Biotechnology 7:257–264

    CAS  Google Scholar 

  • Tingey WM, Gibson RW (1978) Feeding and mobility of the potato leafhopper impaired by glandular trichomes. J Econ Entomol 71:856–858

    Google Scholar 

  • Tingey WM, Laubengayer JE (1981) Aspects of defense against the green peach aphid and potato leafhopper by glandular trichomes of Solanum berthaultii. J Econ Entom 74:721–725

    Google Scholar 

  • Wright RJ, Dimock MB, Tingey WM, Plaisted RL (1985) Colorado potato beetle (Coleoptera Chysomelidae): Expression of resistance in Solanum berthaultii and interspecific potato hybrids. J Econ Entomol 78:576–582

    Google Scholar 

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Authors and Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 252 Emerson Hall, 14853-1902, Ithaca, NY, USA

    Z. Lentini, E. D. Earle & R. L. Plaisted

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  1. Z. Lentini
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  2. E. D. Earle
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  3. R. L. Plaisted
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Communicated by A. R. Hallauer

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Lentini, Z., Earle, E.D. & Plaisted, R.L. Insect-resistant plants with improved horticultural traits from interspecific potato hybrids grown in vitro. Theoret. Appl. Genetics 80, 95–104 (1990). https://doi.org/10.1007/BF00224021

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  • DOI: https://doi.org/10.1007/BF00224021

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