Abstract
Random Amplified Polymorphic DNA (RAPD) was used to generate molecular markers to trace the origin of the fodder legume tagasaste (Chamaecytisus proliferus (L. fil.) Link ssp. palmensis (H. Christ) Kunkel) in the Canary Islands. Results from multivariate analyses of data through “Two Way Indicator Species Analysis” (TWINSPAN) and “Detrended Correspondence Analysis” (DECORANA) showed that genotypes collected on the island of La Palma exhibited a wider range of variation than those from the other islands. This supports the existing hypothesis that tagasaste originated on La Palma and emphasizes the importance of conserving and evaluating germ plasm from this island.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Acebes-Ginovés JR, Del Arco-Aguilar M, Wildpret de la Torre W (1991) Revision taxonómica del género Chamaecytisus Link en Canarias. Vieraea 20:191–202
Francisco-Ortega J (1992) An ecogeographical study within Chamaecytisus proliferus (L. fil.) Link complex (Fabaceae: Genisteae) in the Canary Islands. PhD thesis, The University of Birmingham, UK
Francisco-Ortega J, Jackson MT, Santos-Guerra A, Fernández-Galván M (1990) Genetic resources of the fodder legumes tagasaste and escobon (Chamaecytisus proliferus (L. fil.) Link sensu lato) in the Canary Islands. FAO/IBPGR Plant Genet Resources Newsl 81/82:27–32
Francisco-Ortega J, Jackson MT, Santos-Guerra A, Fernández-Galván M (1991) Historical aspects of the origin and distribution of tagasaste (Chamaecytisus proliferus (L.fil.) Link ssp. palmensis (Christ) Kunkel), a fodder tree from the Canary Islands. J Adelaide Bot Gard 14:67–76
Francisco-Ortega J, Jackson MT, Socorro-Monzón AR, Ford-Lloyd BV (1992a) Ecogeographical characterization of germ plasm of tagasaste and escobon (Chamaecytisus proliferus (L. fil.) Link sensu lato) from the Canary Islands: soil, climatological and geographical features. Invest Agric Prod Veg (in press)
Francisco-Ortega J, Jackson MT, Santos-Guerra A, Fernández-Galván M, Ford-Lloyd, BV (1992b) The phytogeography of the Chamaecytisus proliferus (L. fil.) Link (Fabaceae: Genisteae) complex in the Canary Islands: a multivariate analysis. Vegetatio (in press)
Francisco-Ortega J, Newbury, HJ, Ford-Lloyd BV, Soriano O (1992c) Tagasaste in the Canary Islands in the 17th century. J Adelaide Bot Gard 7(3):377–388
Gauch GG (1982) Multivariate analysis in community ecology. Cambridge University Press, Cambridge
Gawel NJ, Jarret RL (1991) A modified CTAB DMA extraction procedure for Musa and Ipomea. Plant Mol Biol Rep 9:262–266
Harlan JR (1983) The scope for collection and improvement of forage plants. In: McIvor JG, Bray RA (eds) Genetic resources of forage plants. Commonwealth Science and Industrial Research Organization, East Melbourne, pp 3–14
Hill MO (1979a) Twinspan a fortran program for arranging multivariate data in an ordered two-way table by classification of the individuals and attributes. Section of Ecology and Systematics, Cornell University, New York
Hill MO (1979b) Decorana a fortran program for detrended correspondence analysis and reciprocal averaging. Section of Ecology and Systematics, Cornell University, New York
Jackson DA, Somers KM (1991) Putting things in order; the ups and downs of detrended correspondence analysis. Am Nat 137:704–712
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Mohler CL (1987) Cornell ecology programs ms-dos microcomputer package. Microcomputer Power, Ithaca, New York
Morris D (1893) Tagasaste (Cytisus prolifer L. var ‘palmensis’ Chr.) Kew Gard Bull Misc Inf 1893:115–117
Newbury HJ, Ford-Lloyd BV (1993) The use of RAPD for assessing variation in plants. Plant Growth Reg 12:43–51
Pérez V (1862a) Agricultura. El Guanche (Santa Cruz de Tenerife) 294
Pérez V (1862b) Tagasaste o escobón de La Palma. El Guanche (Santa Cruz de Tenerife) 299
Pérez-Ventoso JV (1892) Le tagasaste (Cytisus proliferus varietas) fourrage important. Semaine Medicale, Paris
Powell W, Phillips MS, McNicol JW, Waugh R (1991) The use of DNA markers to estimate the extent and nature of genetic variability in Solatium tuberosum cultivars. Ann Appl Biol 118:423–432
Reghunath BR, Francisco-Ortega J, Newbury HJ, Ford-Lloyd BV (1993) Methods for increasing the efficiency of seed germination in the fodder legumes tagasaste and escobon (Chamaecytisus proliferus (L. fil.) Link sensu lato). Seed Sci Technol 21:225–235
Wilde J, Waugh R, Powell W (1992) Genetic fingerprinting of Theobroma clones using randomly amplified polymorphic DNA markers. Theor Appl Genet 83:871–877
Williams JG, Kubelik AR, Livak KJ, Rafalski LA, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535
Wishart D (1987) CLUSTAN, user manual, 4th edn. University of St. Andrews, St. Andrews, Scotland
Author information
Authors and Affiliations
Additional information
Communicated by J. W Snape
Rights and permissions
About this article
Cite this article
Francisco-Ortega, J., Newbury, H.J. & Ford-Lloyd, B.V. Numerical analyses of RAPD data highlight the origin of cultivated tagasaste (Chamaecytisus proliferus ssp. palmensis) in the Canary Islands. Theoret. Appl. Genetics 87, 264–270 (1993). https://doi.org/10.1007/BF00223775
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223775