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Effects of phospholipases, proteases and neuraminidase on γ-hydroxybutyrate binding sites

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Summary

γ-Hydroxybutyric acid (GHB) is a natural compound of mammalian brain synthesized from GABA. The characteristics of its synthesis, transport, release, distribution and turnover, in addition to the presence of a high affinity binding site for this substance in brain are in favor of a modulator role for GHB. The effects of hydrolytic enzymes on the specific binding capacity of GHB have been studied in the present work. Phospholipases A2 and C, neuraminidase and Pronase markedly decrease GHB binding to crude synaptosomal membranes from rat brain. This effect is time and enzyme concentration dependent. Trypsin, under the conditions employed, is less active. The inhibitory effects of phospholipases is correlated with phospholipid hydrolysis. Lysophospholipids, in the absence of bovine fatty acid free serum albumin partially inhibit GHB binding. The action of neuraminidase has been followed by sialic acid release and modifications of the ganglioside profile. The effects of phospholipase C and of neuraminidase are completely different to those on GABA binding sites. These results represent further data concerning the molecular existence of specific GHB binding sites on rat brain membranes.

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Abbreviations

GHB:

γ-hydroxybutyrate

LPC:

L-α-lysophosphatidylcholine

LPE:

Lysophosphatidylethanolamine

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

BSA:

Bovine Serum Albumin

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Authors and Affiliations

  1. Centre de Neurochimie du CNRS and INSERM U44, 5, rue Blaise Pascal, 67084, Strasbourg cedex, France

    Viviane Hechler, Marcel Mersel, Henri Dreyfus & Michel Maitre

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  1. Viviane Hechler
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  2. Marcel Mersel
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  3. Henri Dreyfus
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  4. Michel Maitre
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Hechler, V., Mersel, M., Dreyfus, H. et al. Effects of phospholipases, proteases and neuraminidase on γ-hydroxybutyrate binding sites. Mol Cell Biochem 93, 87–94 (1990). https://doi.org/10.1007/BF00223496

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  • DOI: https://doi.org/10.1007/BF00223496

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