Abstract
Quantitative trait loci (QTLs) for resistance to pathogen populations of Scelerospora graminicola from India, Nigeria, Niger and Senegal were mapped using a resistant x susceptible pearl millet cross. An RFLP map constructed using F2 plants was used to map QTLs for traits scored on F4 families. QTL analysis was carried out using the interval mapping programme Mapmaker/QTL. Independent inheritance of resistance to pathogen populations from India, Senegal, and populations from Niger and Nigeria was shown. These results demonstrate the existence of differing virulences in the pathogen populations from within Africa and between Africa and India. QTLs of large effect, contributing towards a large porportion of the variation in resistance, were consistently detected in repeated screens. QTLs of smaller and more variable effect were also detected. There was no QTLs that were effective against all four pathogen populations, demonstrating that pathotype-specific resistance is a major mechanism of downy mildew resistance in this cross. For all but one of the QTLs, resistance was inherited from the resistant parent and the inheritance of resistance tended to be the result of dominance or over-dominance. The implications of this research for pearl millet breeding are discussed.
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Appadurai R, Parambaramani, C, Natarajan, US (1975) Note on the inheritance of susceptibility of pearl Imillet to downy-mildew. Indian J Agric Sci 45:179–180
Ball SL (1983) Pathogenic variability of downy mildew (Sclerospora graminicola) on pearl millet. I. Host cultivar reactions to infection by different pathogen isolates. Ann Appl Biol 102:257–264
Ball SL, Pike DJ (1984) Intercontinental variation of Sclerospora graminicola. Ann Appl Biol 104:41–51
Ball SL, Pike DJ, Burridge CY (1986) Characterisation of populations of Sclerospora graminicola. Ann Appl Biol 108:519–526
Basavaraju R, Safeeulla KM, Murty BR (1981a) Inheritance of resistance to downy mildew in pearl millet. Ind J Genet PI Breed 41:144–149
Basavaraju R, Safeeulla KM, Murty BR (1981b) Genetic variance and heritability for resistance to downy mildew in pearl millet. Ind J Genet PI Breed 41:137–143
Bhat S (1973) Investigations on the biology and control of Sclerospora graminicola on bajra. Ph D thesis, Department of Botany, University of Mysore, India
Bubeck DM, Goodman MM, Beavis WD, Grant D (1993) Quantitative trait loci controlling resistance to gray leaf spot in maize. Crop Sci 33:838–847
Dass S, Kapoor RL, Paroda RS, Jatasra DS (1984) Gene effects for downy mildew (Sclerospora graminicola) resistance in pearl millet. Ind J Genet 44:280–285
Dave HR (1987) Pearl millet hybrids. In:Witcombe JR, Beckerman SR (eds) Proceedings of the international pearl millet workshop, 7–11 April 1986. ICRISAT (International Crops Research Institute for the Semi-Arid Tropics), Patancheru, India, pp 121–126
Day PR (1974) Genetics of host-parasite interactions. W.H. Freeman and Company, San Fransisco
De Wit PJGM (1992) Molecular characterisation of gene-for-gene systems in plant-fungus interactions and the application of avirulence genes in the control of plant pathogens. Annu Rev Phytopathol 30:391–418
Dirlewanger E, Isacc PG, Ranade S, Belajouza M, Cousin R, de Vienne D (1994) Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and development developmental traits in Pisum sativum L. Theor Appl Genet 88:17–27
Freymark PJ, Lee M, Woodman WL, Martinson CA (1993) Quantitative and qualitative trait loci affecting host-response to Exserohilum turcicum in maize (Zea mays L.) Theor Appl Genet 87:537–544
Gill KS, Phul PS, Chahal SS, Singh NB (1978) Inheritance of resistance to downy mildew disease in pearl millet. Cereal Res Commun 6:71–74
Girard JC (1975) Downy mildew of pearl millet in Senegal. In: Williams RJ (ed) Proceedings of the consultants' group meetings on downy mildew and ergot of pearl millet. 1–3 October, ICRISAT (International Crops Research Institute for The SemiArid Tropics), Patancheru, India, pp 59–73
Hash CT (1986) Breeding for large grain size in pearl millet, Pennisetum americanum (L.) Leeke, Ph D thesis, Cornell University, USA
ICRISAT (1989) Virulence of Sclerospora graminicola. ICRISAT (International Crops Research Institute for the Semi-Arid Tropics). Annual Report 1988. ICRISAT, Patancheru, India, pp 30–33
Kreike CM, Koning JRA de, Vinke JH, Oijen JW van, Gebhardt C, Stiekema WJ (1993) Mapping of loci involved in quantitatively inherited resistance to the potato cyst-nematode Globodera rostochiensis pathotype Rol. Theor Appl Genet 87:464–470
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Leonards-Schippers C, Geiffers W, Schäfer-Pregl R, Ritter E, Knapp SJ, Salamini F, Gebhardt C (1994) Quantitative resistance to Phytophthora infestans in potato: A case study for QTL mapping in an allogamous plant species. Genetics 137:67–77
Liu CJ, Witcombe JR, Pittaway TS, Nash M, Hash CT, Busso CS, Gale MD (1994) An RFLP-based genetic map of pearl millet (Pennisetum glaucum). Theor Appl Genet 89:481–487
Mehta N, Dang JK (1987) Studies on the inheritance of downy mildew, ergot and smut of pearl millet. Indian J Mycol Pl Pathol 17:200–203
Michelmore R, Pawar MN, Williams RJ (1982) Heterothallism in Sclerospora graminicola. Phytopathology 72:1368–1372
Nienhuis J, Helentjaris T, Slocum M, Ruggero B, Schaefer A (1987) Restriction fragment length polymorphism analysis of loci associated with insect resistance in tomato. Crop Sci 27:797–803
Pè ME, Gianfranceschi L, Taramino G, Tarchini R, Angelini P, Dani M, Binelli G (1993) Mapping quantitative trait loci (QTLs) for resistance to Giberella zeae infection in maize. Mol Gen Genet 241:11–16
Pederson WI, Leath S (1988) Pyramiding major genes for resistance to maintain residual effects. Annu Rev Phytopathol 26:369–378
Pethani KV, Kapoor RL, Chandra S (1980) Gene action and phenotypic stability for incidence of downy mildew disease in pearl millet. Indian J Agric Res 14:217–223
Purseglove JW (1976) Millets. In:Simmonds NW (ed) Evolution of crop plants. Longman, London New York, pp 91–93
Shetty HS, Ahmad R (1981) Physiologic specialisation in Sclerospora graminicola. Indian Phytopathol 34:307–309
Shinde RB, Patil FB, Sangave RA (1984) Resistance to downy mildew in pearl millet. J Maharashtra Agric Univ 9:337–338
Singh F, Singh RK, Singh RM, Singh RB (1978) Genetic analysis of downy mildew (Sclerospora graminicola) resistance in pearl millet [Pennisetum typhoides (Burm.) S. & H.]. Z Pflanzenzüchtg 81:54–59.
Singh F, Singh RM, Singh RB, Singh RK (1980) Genetic studies of downy mildew resistance in pearl millet. In: Gupta VP, Minocha JL (eds) Trends in genetical research of pennisetums. Wesley Press, Ludhiana, pp 171–172
Singh SD, Singh G (1987) Resistance to downy mildew in pearl millet hybrid NHB-3. Indian Phytopathol 40:178–180
Thakur DP (1987) Studies on downy mildew epiphytotic in pearl millet hybrid BJ-104 in Haryana. Haryana Agric Univ J Res 17:68–74
Thakur RP, Shetty KG, King SB (1992) Selection for host-specific virulence in asexual populations of Sclerospora graminicola. Plant Pathol 41:626–632
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Jones, E.S., Liu, C.J., Gale, M.D. et al. Mapping quantitative trait loci for downy mildew resistance in pearl millet. Theoret. Appl. Genetics 91, 448–456 (1995). https://doi.org/10.1007/BF00222972
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DOI: https://doi.org/10.1007/BF00222972