Abstract
We have demonstrated that sorghum DNA sequences of mitochondrial origin can be used to distinguish different male-sterility-inducing cytoplasms. Six DNA clones containing single-copy mitochondrial sequences were hybridized on Southern blots to restriction enzyme-digested DNA of 28 sorghum lines representing sources of different cytoplasmic male-sterility (CMS) groups. Four cytoplasmic types were defined on the basis of the pattern of DNA fragments detected. Similar analyses of 50 additional diverse sorghum accessions suggested that three of the four cytoplasmic types may be diagnostic for CMS. Also, three other cytoplasmic types were discovered. These and other mitochondrial DNA clones may be useful molecular tools for “fingerprinting” sterility-inducing cytoplasms in breeding programs, determining cytoplasmic diversity among germ plasm accessions, and identifying new sources of cytoplasm that induce male sterility.
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References
Bailey-Serres J, Dixon LK, Liddell AD, Leaver CJ (1986) Nuclearmitochondrial interactions in cytoplasmic male-sterile Sorghum. Theor Appl Genet 73:252–260
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Chase DC, Pring DR (1986) Properties of the linear N1 and N2 plasmid-like DNAs from mitochondria of cytoplasmic male-sterile Sorghum bicolor. Plant Mol Biol 6:53–64
Conde MF, Pring DR, Schertz KF, Ross WM (1982) Correlation of mitochondrial DNA restriction endonuclease patterns with sterility expression in six male-sterile sorghum cytoplasms. Crop Sci 22:536–539
Feinberg AP, Vogelstein B (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13
Hussaini SH, Rao PV (1964) A note on the spontaneous occurrence of cytoplasmic male sterility in Indian sorghums. Sorghum Newsl 27:27–28
Kaul MLH (1988) Male sterility in higher plants. (Monographs on theoretical and applied genetics, vol 10). Springer, Berlin, Heidelberg, New York
Maniatis F, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Mittal SP, Swarup V, Joshi AB (1958) Cytoplasmic male sterility in Jowar (Sorghum vulgare Pers). Curr Sci 27:314
Mullen JA, Pring DR, Kempken F, Ferguson F, Ferguson J, Chase CD (1992) Sorghum mitochondrial atp6: divergent amino extensions to a conserved core polypeptide. Plant Mol Biol 20:71–79
Murray MG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
Nagur T, Menon PM (1974) Characterization of different male sterility-inducing cytoplasms in sorghum. Sorghum Newsl 17:18
Pring DR, Conde MF, Schertz KF (1982) Organelle genome diversity in sorghum: male-sterile cytoplasms. Crop Sci 22:414–421
Pring DR, Gengenbach BG, Wise RP (1988) Recombination is associated with polymorphism of the mitochondrial genomes of maize and sorghum. Philos Trans R Soc London Ser B 319: 187–198
Quinby JR (1980) Interaction of genes and cytoplasms in male sterility in sorghum. In: H. D. Loden and D. Wilkonson (eds) American seed Trade association. Proc 35th Annu Corn Sorghum Res Conf., Chicago, Ill., pp 175–184
Rao NGP (1962) Occurrence of cytoplasmic-genetic male sterility in some Indian sorghums. Indian J Genet Plant Breed 22:257–259
Rosenow DT, Schertz KF, Sotomayor A (1980) Germplasm release of three pairs (A and B) of sorghum lines with A2 cytoplasmicgenetic sterility system. Texas Agricultural Experiment Station, MP-1448:1–2
Ross WM (1965) Cytoplasmic male sterility and fertility restoration of some major sorghum groups. In: E. Hang (ed) Natural Biam Sorghum producers association, Amarillo, Texas. Proc Grain Sorg Res Util Conf. pp 57–62
Ross WM, Hackerott HL (1972) Registration of seven isocytoplasmic sorghum germ plasm lines. Crop Sci 12:720–721
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. Proc Natl Acad Sci USA 81:8014–8018
Schertz KF (1977) Registration of A2 Tx 2753 and BTx 2753 sorghum germ plasm (Reg. No. GP 30 and 31). Crop Sci 17:983
Schertz KF, Pring DR (1982) Cytoplasmic sterility systems in sorghum. In: House LR, Mughogho LK, Peacock JM (eds) Sorghum in the eighties. ICRISAT, Patancheru, India, pp 373–383
Schertz KF, Ritchey JM (1978) Cytoplasmic-genic male-sterility systems in Sorghum. Crop Sci 18:890–893
Schertz KF, Sotomayor-Rios A, Torres-Cardona S (1989) Cytoplasmic-nuclear male sterility-opportunities in breeding and genetics. In: E. Hang (Ed.) Natural Biam Sorghum producers association. Proc 16th Biennial Grain Sorghum Res Utilization Conf. Lubbock, Tex pp 175–186
Stephens JG, Holland RF (1954) Cytoplasmic male-sterility for hybrid sorghum seed production. Agron J 46:20–23
Tripathi DB, Mehta SL, Rana BS, Rao NGP (1980) Characterization of diverse cytoplasmic genetic male steriles in sorghum (Sorghum bicolor L. Moench). Sorghum Newsl 23:107–108
Webster OJ, Singh SP (1964) Breeding behavior and histological structure of a nondehiscent anther character in Sorghum vulgare Pers. Crop Sci 4:656–658
Worstell JV, Kidd HJ, Schertz KF (1984) Relationships among male sterility inducing cytoplasms of sorghum. Crop Sci 24:186–189
Xu GW, Magill CW, Schertz KF, Hart GE (1994) A RFLP linkage map of Sorghum bicolor (L.) Moench. Theor Appl Genet 89:139–145
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Xu, GW., Cui, YX., Schertz, K.F. et al. Isolation of mitochondrial DNA sequences that distinguish male-sterility-inducing cytoplasms in Sorghum bicolor (L.) Moench. Theoret. Appl. Genetics 90, 1180–1187 (1995). https://doi.org/10.1007/BF00222941
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DOI: https://doi.org/10.1007/BF00222941