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Asymmetric somatic hybridization between tomato (Lycopersicon esculentum Mill) and gamma-irradiated potato (Solanum tuberosum L.): a quantitative analysis

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Abstract

We analyzed 110 asymmetric fusion products, obtained by fusion of hygromycin-resistant tomato protoplasts and gamma-irradiated kanamycin-resistant potato protoplasts that expressed β-glucuronidase (GUS). The fusion products were selected for resistance to both antibiotics, and were subsequently analyzed for their shoot regeneration potential, GUS activity, expression of two potato isoenzymes, chloroplast type, total genomic DNA content, and relative genomic composition. No viable plants could be obtained and the calli were highly polypoid. All hybrids expressed GUS activity, whereas they displayed a large variation with respect to the other traits.

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References

  • Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9:208–218

    CAS  Google Scholar 

  • Bates GW (1990) Asymmetric hybridization between Nicotiana tabacum and N. repanda by donor recipient protoplast fusion: transfer of TMV resistance. Theor Appl Genet 80:481–487

    Google Scholar 

  • Bonnema AB, Melzer JM, Murray LW, O'Connell MA (1992) Non-random inheritance of organellar genomes in symmetric and asymmetric somatic hybrids between Lycopersicon esculentum and L. pennellii. Theor Appl Genet 84:435–442

    Google Scholar 

  • Derks FHM, Wijbrandi J, Koornneef M, Colijn-Hooymans CM (1991) Organelle analysis of symmetric and asymmetric hybrids between Lycopersicon peruvianum and Lycopersicon esculentum. Theor Appl Genet 81:199–204

    Google Scholar 

  • Derks FHM, Hakkert JC, Verbeek WHJ, Colijn-Hooymans CM (1992) Genome composition of asymmetric hybrids in relation to the phylogenetic distance between the parents. Nucleus-chloroplast interaction. Theor Appl Genet 84:930–940

    Google Scholar 

  • Dudits D, Fejér O, Hadlaczky G, Koncz C, Lázár GB, Horváth G (1980) Intergeneric gene transfer mediated by plant protoplast fusion. Mol Gen Genet 179:283–288

    Google Scholar 

  • Dudits D, Maroy E, Praznovsky T, Olah Z, Gyorgyey J, Cella R (1987) Transfer of resistance traits from carrot into tobacco by asymmetric somatic hybridization: regeneration of fertile plants. Proc Natl Acad Sci USA 84:8434–8438

    Google Scholar 

  • Gleba YY, Hinnisdaels S, Sidorov VA, Kaleda VA, Parokonny AS, Boryshuk NV, Cherep NN, Negrutiu I, Jacobs M (1988) Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gammafusion”. Theor Appl Genet 76:760–766

    Google Scholar 

  • Hinnisdaels S, Bariller L, Mouras A, Sidorov V, Del-Favero J, Veuskens J, Negrutiu I, Jacobs M (1991) Highly-asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after “gamma”-fusion. Theor Appl Genet 82:609–614

    Google Scholar 

  • Imamura J, Saul MW, Potrykus I (1987) X-ray irradiation-promoted asymmetric somatic hybridisation and molecular analysis of the products. Theor Appl Genet 74:445–450

    Google Scholar 

  • Itoh K, Futsuhara Y (1983) Interspecific transfer of only part of genome by fusion between non-irradiated protoplasts of Nicotiana glauca and X-ray-irradiated protoplasts of N. langsdorffii. Jpn J Genet 58:545–553

    Google Scholar 

  • Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907

    CAS  PubMed  Google Scholar 

  • Koornneef M, Hanhart CJ, Martinelli L (1987a) A genetic analysis of cell-culture traits in tomato. Theor Appl Genet 74:633–641

    Google Scholar 

  • Koornneef M, Jongsma M, Weide R, Zabel P, Hille J (1987b) Transformation of tomato. In: Nevins DJ, Jones RA (eds) Tomato biotechnology. Alan R Liss Inc, New York, pp 169–178

    Google Scholar 

  • Koornneef M, Bade J, Hanhart C, Horsman K, Schel J, Soppe W, Verkerk R, Zabel P (1993) Characterization and mapping of a gene controlling shoot regeneration in tomato. The Plant J 3:131–141

    Google Scholar 

  • Levi A, Ridley BL, Sink KC (1988) Biased organelle transmission in somatic hybrids of Lycopersicon esculentum and Solanum lycopersicoides. Curr Genet 14:177–182

    Google Scholar 

  • Li Y, Sink KC (1992) Cell type determines plastid transmission in tomato intergeneric somatic hybrids. Curr Genet 22:167–171

    Google Scholar 

  • Melzer JM, O'Connell MA (1992) Effect of radiation dose on the production of and the extent of asymmetry in tomato asymmetric somatic hybrids. Theor Appl Genet 83:337–344

    Google Scholar 

  • Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100:487–495

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    CAS  Google Scholar 

  • O'Connell MA, Hanson MR (1985) Somatic hybridization between Lycopersicon esculentum and Solanum pennellii. Theor Appl Genet 70:1–12

    Google Scholar 

  • Parokonny AS, Kenton AY, Gleba YY, Bennett MD (1992) Genome reorganization in Nicotiana asymmetric somatic hybrids analysed by in-situ hybridization. The Plant J 2:863–874

    Google Scholar 

  • Pehu E, Karp A, Moore K, Steele S, Dunckley R, Jones MGK (1989) Molecular, cytogenetic and morphological characterization of somatic hybrids of diploid Solanum tuberosum and diploid S. brevidens. Theor Appl Genet 78:696–704

    Google Scholar 

  • Persson AR (1960) Physiological and genetical investigations on chlorophyll mutants. Rep Tomato Genet Coop 10:27–28

    Google Scholar 

  • Piastuch WC, Bates GW (1990) Chromosomal analysis of Nicotiana asymmetric somatic hybrids by dot blotting and insitu hybridization. Mol Gen Genet 222:97–103

    Google Scholar 

  • Sacristan MD, Gerdemann-Knörck, Schieder O (1989) Incorporation of hygromycin resistance in Brassica nigra and its transfer to B. napus through asymmetric protoplast fusion. Theor Appl Genet 78:194–200

    Google Scholar 

  • Schiller B, Herrmann RG, Melchers G (1982) Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids. Mol Gen Genet 186:453–459

    Google Scholar 

  • Schoenmakers HCH, Nobel EM, Koornneef M (1992) Somatic hybridization between nitrate-reductase-deficient mutants of tomato (Lycopersicon esculentum Mill.) and wild-type potato (Solanum tuberosum L.) Plant Cell Tissue Org Cult 31:151–154

    Google Scholar 

  • Schoenmakers HCH, Wolters AMA, Nobel EM, Klein CMJ de, Koornneef M (1993) Allotriploid somatic hybrids of diploid tomato (Lycopersicon esculentum Mill.) and monoploid potato (Solanum tuberosum L.) Theor Appl Genet (in press)

  • Sjödin C, Glimelius K (1989) Transfer of resistance against Phoma Ungarn to Brassica napus by asymmetric somatic hybridization combined with toxin selection. Theor Appl Genet 78:513–520

    Google Scholar 

  • Somers DA, Naterayan KR, Kleinhofs A, Cooper-Bland S, Cocking EC (1986) Immunological evidence for transfer of the barley nitrate reductase structural gene to Nicotiana tabacum by protoplast fusion. Mol Gen Genet 204:296–301

    Google Scholar 

  • Vallejos CE (1983) Enzyme activity staining. In: Tanksley SD, Orton TJ (eds), Isozymes in plant genetics and breeding, part A. Elsevier, Amsterdam, pp 469–515

    Google Scholar 

  • Visser RGF, Hoekstra R, Van der Leij FR, Pijnacker LP, Witholt B, Feenstra WJ (1988) In-situ hybridization to somatic metaphase chromosomes of potato. Theor Appl Genet 76:420–424

    Google Scholar 

  • Vries SE de, Ferweda MA, Loonen AEHM, Pijnacker LP, Feenstra WJ (1987) Chromosomes in somatic hybrids between Nicotiana plumbaginifolia and monoploid potato. Theor Appl Genet 75:170–176

    Google Scholar 

  • Wolters AMA, Schoenmakers HCH, van der Meulen-Muisers JJM, van der Knaap E, Derks FHM, Koornneef M, Zelcer A (1991) Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum. Theor Appl Genet 83:225–232

    Google Scholar 

  • Wolters AMA, Koornneef M, Gilissen LJW (1993) The chloroplast and the mitochondrial DNA type are correlated with the nuclear composition of somatic hybrid calli of Solanum tuberosum and Nicotiana plumbaginifolia. Curr Genet 24:260–267

    Google Scholar 

  • Wijbrandi J, Zabel P, Koornneef M (1990a) Restriction fragment length polymorphism analysis of somatic hybrids between Lycopersicon esculentum and irradiated L. Peruvianum: evidence for limited donor genome elimination and extensive chromosome rearrangements. Mol Gen Genet 222: 270–277

    Google Scholar 

  • Wijbrandi J, Wolters AMA, Koornneef M (1990b) Asymmetric somatic hybrids between Lycopersicon esculentum and irradiated Lycopersicon peruvianum. Theor Appl Genet 80: 665–672

    Google Scholar 

  • Yamashita Y, Terada R, Nishibayashi S, Shimamoto K (1989) Asymmetric somatic hybrids of Brassica: partial transfer of B. campestris genome into B. oleracea by cell fusion. Theor Appl Genet 77:189–194

    Google Scholar 

  • Zabel P, Meyer D, Van de Stolpe O, Van der Zaal B, Ramanna MS, Koornneef M, Krens F, Hille J (1985) Towards the construction of artificial chromosomes for tomato. In: van Vloten-Doting L, Groot GSP, Hall TC (eds), Molecular form and function of the plant genome. Plenum, New York, pp 609–624

    Google Scholar 

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Authors and Affiliations

  1. Department of Genetics, Agricultural University Wageningen, Dreijenlaan 2, NL-6703, HA Wageningen, the Netherlands

    H. C. H. Schoenmakers, A. -M. A. Wolters, A. de Haan, A. K. Saiedi & M. Koornneef

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  1. H. C. H. Schoenmakers
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  2. A. -M. A. Wolters
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  3. A. de Haan
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  4. A. K. Saiedi
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  5. M. Koornneef
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Communicated by I. Potrykus

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Schoenmakers, H.C.H., Wolters, A.M.A., de Haan, A. et al. Asymmetric somatic hybridization between tomato (Lycopersicon esculentum Mill) and gamma-irradiated potato (Solanum tuberosum L.): a quantitative analysis. Theoret. Appl. Genetics 87, 713–720 (1994). https://doi.org/10.1007/BF00222897

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  • DOI: https://doi.org/10.1007/BF00222897

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