Skip to main content
SpringerLink
Log in

Immunocytochemical evidence for the presence of oxytocin in rat testis

  • Short Communications
  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The presence of oxytocin, vasopressin and neurophysin in the testis of adult Wistar and Brattleboro rats has been examined immunocytochemically. After fixation in modified Bouin's solution, or Bouin's sublimate fixative, immunostaining was accomplished with the peroxidase-antiperoxidase method. The presence of immunoreactive oxytocin was demonstrated in 80% of the interstitial cell population of both rat strains while no staining was observed for vasopressin or neurophysin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Guldenaar SEF, Wathes DC, Pickering BT (1984) Immunocytochemical evidence for the presence of oxytocin and neurophysin in the large cells of the bovine corpus luteum. Cell Tissue Res 237:349–352

    Google Scholar 

  • Knorr DW, Vanha-Pertulla T, Lipsett MB (1970) Structure and function of rat testis through pubescence. Endocrinology 86:1298–1304

    Google Scholar 

  • Nicholson HD, Swann RW, Burford GD, Wathes DC, Porter DG, Pickering BT (1984) More steroidogenic tissues containing oxytocin and vasopressin: human testis and fetal adrenal. Regul Pept 8:141–146

    Google Scholar 

  • Sofroniew MV, Schrell U (1982) Long-term storage and regular repeated use of diluted antisera in glass staining jars for increased sensitivity, reproducibility, and convenience of singleand two-color light microscopic immunocytochemistry. JHistochem Cytochem 30:504–511

    Google Scholar 

  • Sternberger L (1979) Immunocytochemistry, 2nd ed. John Wiley and Sons, New York, Chichester, Brisbane, Toronto

    Google Scholar 

  • Swaab DF, Pool CW (1975) Specificity of oxytocin and vasopressin immunofluorescence. J Endocrinol 66:263–272

    Google Scholar 

  • Swann RW, O'Shaughnessy PJ, Birkett SD, Wathes DC, Porter DG, Pickering BT (1984) Biosynthesis of oxytocin in the corpus luteum. FEBS Lett 174:262–266

    Google Scholar 

  • Wathes DC, Swann RW (1982) Is oxytocin an ovarian hormone? Nature 297:225–227

    Google Scholar 

  • Watkins WB (1983) Immunohistochemical localization of neurophysin and oxytocin in the sheep corpora lutea. Neuropeptides 7:51–54

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Anatomy, The Medical School, University of Bristol, Bristol, UK

    S. E. F. Guldenaar & B. T. Pickering

Authors
  1. S. E. F. Guldenaar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. T. Pickering
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Guldenaar, S.E.F., Pickering, B.T. Immunocytochemical evidence for the presence of oxytocin in rat testis. Cell Tissue Res. 240, 485–487 (1985). https://doi.org/10.1007/BF00222364

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00222364

Key words

Search

Navigation