Summary
Examination of the surface topography of canine gallbladder epithelium using the scanning electron microscope reveals subtle changes in the morphology of the cellular components between the full and empty states. In the full state large bays exist between relatively low, ridge-like crests or folds of mucosa which gradually increase in height as the gallbladder empties causing a subsequent reduction in size and volume of the bays. The relaxed gallbladder exhibits an irregular arrangement of tall, closely apposed mucosal folds with intervening deep valleys. The degree of apical convexity of individual epithelial cells will increase as the mucosal folds begin to relax. Other mucosal features most easily observed in the full state were small pit-like intraepithelial glands. The apical surfaces of the epithelial cells exhibit a densely uniform microvillus border and occasional membrane-bounded cytoplasmic protrusions (apical bullae) extending out into the lumen of the organ. The lateral cell membranes and basal lamina were also examined. Correlative methacrylate and transmission electron microscopic findings are included. The functional aspects of these morphological alterations and apical protrusions are discussed.
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Bader G (1965a) Die submikroskopische Struktur des Gallenblasenepithels und seine Regeneration. I. Mitt. Karpfen (Cyprinus carpio) und Frosch (Rana esculenta L.) Z mikrosk anat Forsch 74:92–107
Bader G (1965b) Die submikroskopische Structur des Gallenblasenepithels. III. Mitt. Das Epithel der Steingallenblase des Menschen. Frankfurt Z Pathol 74:502–511
Bryn DHP de (1964) The fine structure of the megakaryocyte of the bone marrow of the guinea pig. Z Zellforsch mikrosk Anat 64:111–118
Castellucci M, Caggiati A (1980) Surface aspects of the rabbit gallbladder mucosa and their functional implications. J Submicrosc Cytol 12:375–390
Dietschy JM (1966) Recent developments in solute and water transport across the gallbladder epithelium. Gastroenterology 50:692–707
Elfving G (1960) Crypt and ducts in the gallbladder wall. Acta Pathol Microbiol Scand 49 (suppl 135): 1–45
Elfving G, Teir H, Degert H, Makela V (1969) Mucosal hyperplasia in the gallbladder demonstrated by plastic models. Acta Pathol Microbiol Scand 77:384–388
Halpert B (1927) Morphological studies on the gallbladder. I. A note on the development and microscopic structure of the normal human gallbladder. Bull Johns Hopkins Hosp 40:117–124
Hayward AF (1965) The fine structure of the gallbladder epithelium of the sheep. Z Zellforsch 65:331–339
Hayward AF (1966) An electron microscopic study of developing gall bladder epithelium in the rabbit. J Anat 100:245–259
Hayward AF (1968) The structure of gallbladder epithelium. Int Rev Gen Exp Zool 3:205–239
Highison GJ, Low FN (1982) Microdissection by ultrasonification after prolonged OsO4 fixation: a technique for scanning electron microscopy. J Submicrosc Cytol 14:161–170
Johnson FR, McMinn RMH, Birchenough RF (1962) The ultrastructure of the gallbladder epithelium of the dog. J Anat 96:477–487
Kaye GI, Maenza RM, Lane N (1966a) Cell replication in rabbit gallbladder. An autoradiographic study of epithelial and associated fibroblast renewal in vivo and in vitro. Gastroenterology 51:670–680
Kaye GI, Wheeler HO, Whitlock RT, Lane N (1966b) Fluid transport in the rabbit gallbladder. A combined physiological and electron microscopic study. J Cell Biol 30:237–268
Kurosumi K (1961) Electron microscopic analysis of the secretion mechanism. Int Rev Cytol 11:1–124
Laitio M, Nevalainen T (1972a) Scanning and transmission electron microscope observations on gallbladder epithelium. I. Adult structure. Z Anat Entwickl Gesch 136:319–325
Laitio M, Nevalainen T (1972b) Scanning and transmission electron microscope observations on human gallbladder epithelium. II. Foetal development. Z Anat Entwickl Gesch 136:326–335
Mathis J (1927) Über Sekretionserscheinungen in Gallengängen. Z mikrosk anat Forsch 10:527–582
McCluggage SG, Low FN (1982) Porosity of the intestinal basal lamina demonstrated by microdissection. J Cell Biol 95:113
McCluggage SG, Low FN (1983) Regional differences in the porosity of basal lamina demonstrated by microdissection. Anat Rec 205:116
Meenan NM, Schiebler TH (1978) Zur Entwicklung des Gallenblasenepithels der Maus. Anat Anz 144:407–428
Mueller JC, Jones AI, Long SA (1972) Topographic and subcellular anatomy of the guinea pig gallbladder. Gastroenterology 63:856–868
Myllarniemi H, Nickels JI (1977) Observations by scanning electron microscopy of normal and pathological human gallbladder epithelium. Acta Pathol Microbiol Scand Sect A 85:42–48
Ott H (1937) Das Gallengangsystem des Schweins. Arch Anat Physiol 107:7–17
Ralph PH (1950) Surface structure of gallbladder and intestinal epithelium of man and monkey. Anat Rec 108:217–220
Simpson LO (1980) Basement membranes and biological thixotropy: a new hypothesis. Pathology 12:377–389
Togari C, Okada T (1954) Cytological observations on the epithelium of the gallbladder in urodele amphibian (Triturus pyrrhogaster). Okajimas Folia Anat Jpn 26:327–334
Tormey J McD, Diamond JM (1967) The ultrastructural route of fluid transport in rabbit gallbladder. J Gen Physiol 50:2031–2060
Tusques J, Senelar R, Gingue Y (1964) Etude histochimique sur les vesicules biliaires de mouton et de cobaye: les mucopolysaccharides. Ann Histochim 9:269–276
Viehberger G (1982) Apical surface of the epithelial cells in the gallbladder of the rainbow trout and the tench. Cell Tissue Res 224:449–454
Wahlin T, Schiebler TH (1975) Zur Entwicklung des Gallenblasenepithels des Meerschweinchens. II. Elektronenmikroskopische und enzymhistochemische Untersuchungen. Histochemistry 44:353–375
Wheeler HO (1971) Concentrating function of the gallbladder. Am J Med 51:588–595
Yamada K (1960) A unique mode of apocrine secretion in the gall bladder epithelium of Clemmys japonica with a note on the histochemical quality of the secretory materai. Okajimas Folia Anat Jpn 35:47–76
Yamada E (1968) Some observations on the fine structure of light and dark cells in the gallbladder epithelium of the mouse. Z Zellforsch 84:463–472
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Support for this study was provided by the Medical Research Council of Canada and a major equipment grant from the Alberta Heritage Foundation for Medical Research. The authors wish to acknowledge the technical assistance provided by Edith Schwaldt and Gary Higgs of the Surgical-Medical Research Institute as well as Greg Morrison, Department of Anatomy
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MacPherson, B.R., Scott, G.W. & Lennon, F. Morphology of canine gallbladder. Cell Tissue Res. 233, 161–174 (1983). https://doi.org/10.1007/BF00222240
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DOI: https://doi.org/10.1007/BF00222240