Summary
The gastrointestinal tract of the alligator Alligator mississipiensis has been investigated for the presence of immunoreactivity to fourteen regulatory peptides all known to occur in the mammalian gut system.
Mucosal endocrine cells reacting specifically with the antisera to neurotensin, C-terminal gastrin, somatostatin, bombesin, secretin, pancreatic glucagon and enteroglucagon were detectable, the distribution of these cells being, in general, similar to the mammalian pattern. Peripheral nerve cell bodies and nerve fibres were detected with the antisera to vasoactive intestinal polypeptide, substance P, bombesin and somatostatin again with a distribution similar to that seen in mammals.
No immunoreactivity was observed with the available antisera to glicentin, motilin, gastric inhibitory polypeptide, gastrin 34, cholecystokinin 9–20 and met-enkephalin.
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References
Buchan AMJ, Polak JM, Pearse AGE (1980) Gut hormones in Salamandra salamandra: An immunocytochemical and electron microscopic investigation. Cell Tissue Res 211:331–343
Buchan AMJ, Lance V, Polak JM (1982a) The endocrine pancreas of Alligator mississipiensis — an immunocytochemical study. Cell Tissue Res 224:117–128
Buchan AMJ, Bryant MG, Stein BA, Gregor M, Ghatei MA, Bloom SR, Polak JM (1982b) Pancreatic glucagon in human foetal stomach. Histochemistry 74:515–520
Carraway R, Bhatnagar YM (1980) Immunochemical characterisation of neurotensin-like peptides in chicken. Peptides 1:159–165
Carraway R, Ruane SE, Kim HR (1982) Distribution and immunochemical character of neurotensin-like material in representative vertebrates and invertebrates — Apparent conservation of the COOH-terminal region during evolution. Peptides 3:115–124
Creagh T, Cannon D, Skranbanek P, Powell D (1980) Phytogeny of substance P. Irish J Med Sci 148:109–110
Dimaline R (1982) Purification and characterisation of a peptide related to gastrin and CCK from the stomach of a reptile Crocodilus niloticus. Presented at the 4th International Symposium on Gastrointestinal Hormones Stockholm Sweden
El-Salhy M, Grimelius L (1981) The endocrine cells of the gastrointestinal mucosa of a squamate reptile, the grass lizard (Mabuya quinquetaeniata). A histological and immunohistochemical study. Biomed Res 2:639–658
Falkmer S, Ebert R, Arnold R, Creutzfeldt W (1980) Some phylogenetic aspects on the enteroinsular axis with particular regard to the appearance of the gastric inhibitory polypeptide. Front Horm Res 7:1–6
Gabe M (1973) Contribution à l'histologie des cellules endocrines duodénales des sauropsides. Acta Anat 85:434–449
Gabe M, Saint Girons H (1972) Contribution à l'histologie de l'estomac des lepidosauriens (Reptiles). Zool Jb 89:579–599
Grimelius L, Polak JM, Solcia E, Pearse AGE (1978) The enteroglucagon cell. In: Bloom SR (ed) Gut hormones Churchill Livingstone Edinburgh, pp 365–367
Larsson LI, Rehfeld JF (1977) Evidence for a common evolutionary origin of gastrin and cholecystokinin. Nature 269:335–338
Larsson LI, Rehfeld JF (1978) Evolution of CCK-like hormones. In: Bloom SR (ed) Gut hormones Churchill Livingstone Edinburgh, pp 68–73
Lechago J, Hohnquist AL, Rosenquist GL, Walsh JH (1978) Localization of bombesin-like peptides in frog gastric mucosa. Gen Comp Endocrinol 36:553–558
Moody AJ, Thim L (1981) The relationship between glucagon and gut glucagon-like immunoreactants (Gut GLIs). In: Polak JM, Bloom SR (eds) Gut hormones 2nd edn Churchill Livingstone Edinburgh, pp 312–319
Pearse AGE, Polak JM (1975) Bifunctional reagents as vapour and liquid phase fixatives for immunohistochemistry. Histochem J 7:179–186
Polak JM, Bloom SR (1979) The neuroendocrine design of the gut. Clin Endocrinol Metabol 1979:313–330
Polak JM, Bloom SR (1982) Localization of regulatory peptides in the gut. Brit Med Bull 38:303–307
Ravazzola M, Unger RH, Orci L (1981) Demonstration of glucagon in the stomach of human fetuses. Diabetes 30:879–882
Reinecke M, Almasan K, Carraway R, Helmstaedter V, Forssmann WG (1980) Distribution patterns of neurotensin-like immunoreactive cells in the gastro-intestinal tract of higher vertebrates. Cell Tissue Res 205:383–395
Reinecke M, Falkmer S, Ryan U, Forssmann WG (1982) Localisation of somatostatin-immunoreactivity (SOM-IR) in enteroendocrine cells and enteric nerves of the vertebrate gut. Presented at the 4th International Symposium on Gastrointestinal Hormones Stockholm Sweden
Said SI (1981) VIP overview. In: Polak JM, Bloom SR (eds) Gut hormones 2nd edn Churchill Livingstone Edinburgh, pp 379–384
Schultzberg M, Hökfelt T, Nilsson G, Terenius L, Rehfeld JF, Brown M, Elde R, Goldstein M, Said S (1980) Distribution of peptide- and catecholamine-containing neurons in the gastro-intestinal tract of rat and guinea-pig: Immunohistochemical studies with antisera to substance P, vasoactive intestinal polypeptide, enkephalins, somatostatin, gastrin/chole-cystokinin, neurotensin and dopamine-hydroxylase. Neuroscience 5:689–774
Sternberger L (1979) Immunocytochemistry 2nd edn Wiley & Sons NY
Timson CM, Polak JM, Wharton J, Ghatei MA, Bloom SR, Usellini L, Capella C, Solcia E, Brown MR, Pearse AGE (1979) Bombesin-like immunoreactivity in the avian gut and its localisation to a distinct cell type. Histochemistry 61:213–221
Van Noorden S, Falkmer S (1980) Gut-islet endocrinology — some evolutionary aspects. Invest Cell Pathol 3:21–36
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Buchan, A.M.J., Lance, V. & Polak, J.M. Regulatory peptides in the gastrointestinal tract of Alligator mississipiensis . Cell Tissue Res. 231, 439–449 (1983). https://doi.org/10.1007/BF00222193
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DOI: https://doi.org/10.1007/BF00222193