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Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification

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Summary

Repetitive DNA sequences present in the grapevine genome were investigated as probes for distinguishing species and cultivars. Microsatellite sequences, minisatellite sequences, tandemly arrayed genes and highly repetitive grapevine sequences were studied. The relative abundance of microsatellite and minisatellite DNA in the genome varied with the repeat sequence and determined their usefulness in detecting RFLPs. Cloned Vitis ribosomal repeat units were characterised and showed length heterogeneity (9.14–12.15 kb) between and within species. A highly repetitive DNA sequence isolated from V. vinifera was found to be specific only to those species classified as Euvitis. DNA polymorphisms were found between Vitis species and between cultivars of V. vinifera with all classes of repeat DNA sequences studied. DNA sequences suitable for DNA fingerprinting gave genotype-specific patterns for all of the cultivars and species examined. The DNA polymorphisms detected indicates a moderate to high level of heterozygosity in grapevine cultivars.

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Authors and Affiliations

  1. Division of Horticulture, CSIRO, GPO Box 350, 5001, Adelaide, SA, Australia

    M. R. Thomas, S. Matsumoto, P. Cain & N. S. Scott

Authors
  1. M. R. Thomas
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  2. S. Matsumoto
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  3. P. Cain
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  4. N. S. Scott
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Additional information

Communicated by J.S. Beckmann

On leave from the Biochemical Research Institute, Nippon Menard Cosmetic Co, Ltd, Ogaki Gifuken, 503 Japan

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Thomas, M.R., Matsumoto, S., Cain, P. et al. Repetitive DNA of grapevine: classes present and sequences suitable for cultivar identification. Theoret. Appl. Genetics 86, 173–180 (1993). https://doi.org/10.1007/BF00222076

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  • DOI: https://doi.org/10.1007/BF00222076

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