Summary
Mitochondrial DNA (mtDNA) variation in natural Beta maritima populations has been characterized by way of Southern blot hybridizations of total DNA using non-radioactive probes and chemiluminescent detection. It was found that the previously described N (“normal”) mitochondrial type could be subdivided into three subtypes. A new mitochondrial genotype (type R) was distinguished in addition to the previously described type S. Both are male-sterile cytoplasms and can produce a. segregation of sexual phenotypes in their progenies depending on the nuclear background. The populations contained at least two to four different mitochondrial genotypes.
Similar content being viewed by others
References
Allefs JJHM, Salentijn EM, Krens FA, Rouwendal GJA (1990) Optimization of non-radioactive Southern blot hybridization: single copy detection and re-use of blots. Nucleic Acids Res 18:3099–3100
Birnboim HC, Doly J (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
Boutin V, Pannenbecker G, Ecke W, Schewe G, Saumitou-Laprade P, Jean R, Vernet Ph, Michaelis G (1987) Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects. Theor Appl Genet 73:625–629
Boutin-Stadler V, Saumitou-Laprade P, Valero M, Jean R, Vernet Ph (1989) Spatio-temporal variation of male-sterile frequencies in two natural populations of Beta maritima. Heredity 63:395–400
Boutry M, Briquet M (1982) Michondrial modifications associated with cytoplasmic male sterility in faba beans. Eur J Biochem 127:129–135
Brears T, Lonsdale DM (1988) The sugar beet mitochondrial genome: a complex organization generated by homologous recombination. Mol Gen Genet 214:514–522
Dawson AJ, Jones VP, Leaver CJ (1984) The apocytochrome b gene in maize mitochondria does not contain introns and is preceded by a potential ribosome binding site. EMBO J 3:2107–2113
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21
Dewey RE, Levings CS III, Timothy DH (1985a) Nucleotide sequence of ATPase subunit 6 gene of maize mitochondria. Plant Physiol 79:914–919
Dewey RE, Schuster AM, Levings CS III, Timothy DH (1985b) Nucleotide sequence of Fo-ATPase proteolipid (subunit 9) gene of maize mitochondria. Proc Natl Acad Sci USA 82:1015–1019
Duchenne M, Lejeune B, Fouillard P, Quetier F (1989) Comparison of the organization and expression of mtDNA of fertile and male-sterile sugar beet varieties (Beta vulgaris L.). Theor Appl Genet 78:633–640
Edwardson JR (1970) Cytoplasmic male sterility. Bot Rev 36:341–420
Halldén C, Bryngelsson T, Bosemark NO (1988) Two new types of cytoplasmic male sterility found in wild Beta beets. Theor Appl Genet 75:561–568
Hanson MR (1991) Plant mitochondrial mutations and male sterility. Annu Rev Genet 25:461–486
Hanson MR, Conde MF (1985) Functioning and variation of cytoplasmic genomes: lessons from cytoplasmic-nuclear interactions affecting male fertility in plants. Int Rev Cytol 94:213–267
Hiesel R, Brennicke A (1983) Cytochrome oxidase subunit II gene in mitochondria of Oenothera has no intron. EMBO J 2:2173–2178
Hiesel R, Schobel W, Schuster W, Brennicke A (1987) The cytochrome oxidase subunit I and subunit III genes in Oenothera mitochondria are transcribed from identical promoter sequences. EMBO J 6:29–34
Isaac PG, Jones VP, Leaver CJ (1985) The maize cytochrome c oxidase subunit I gene: sequence, expression and rearrangement in cytoplasmic male-sterile plants. EMBO J 4:1617–1623
Kaul MLH (1988) Male sterility in higher plants. Springer, Berlin Heidelberg New York
Köhler RH, Horn R, Lössl A, Zetsche K (1991) Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene. Mol Gen Genet 227:369–376
Kolodner R, Tewari KK (1975) The molecular size and conformation of the chloroplast DNA from higher plants. Biochim Biophys Acta 402:372–390
Komarnitsky IK, Samoylov AM, Red'ko VV, Peretyayko VG, Gleba YY (1990) Intraspecific diversity of sugar beet (Beta Vulgaris) mitochondrial DNA. Theor Appl Genet 80:253–257
Levings CS III (1990) The Texas cytoplasm of maize: cytoplasmic male sterility and disease susceptibility. Science 250:942–947
Lonsdale DM, Hodge TP, Fauron CM-R (1984) The physical map and organisation of the mitochondrial genome from the fertile cytoplasm of maize. Nucl Acids Res 12:9249–9261
Mackenzie SA, Chase CD (1990) Fertility restoration is associated with loss of a portion of the mitochondrial genome in cytoplasmic male-sterile common bean. Plant Cell 2:905–912
Makaroff CA, Apel IJ, Palmer JD (1991) The role of coxI-associated repeated sequences in plant mitochondrial DNA rearrangements and radish cytoplasmic male sterility. Curr Genet 19:183–190
Mann V, McIntosh L, Theurer C, Hirschberg J (1989) A new cytoplasmic male-sterile genotype in the sugar beet Beta vulgaris L.: a molecular analysis. Theor Appl Genet 78:293–297
Mikami T, Kishima Y, Sugiura M, Kinoshita T (1985) Organelle genome diversity in sugar beet with normal and different sources of male sterile cytoplasms. Theor Appl Genet 71:166–171
Nivison HT, Hanson MR (1989) Identification of a mitochondrial protein associated with cytoplasmic male sterility in Petunia. Plant Cell 1:1121–1130
Owen FV (1942) Male sterility in sugar beets produced by complementary effects of cytoplasmic and Mendelian inheritance (abstr) Am J Bot 29:692
Owen FV (1952) Mendelian male sterility in sugar beet. Proc Am Sugar Beet Technol 372–376
Palmer JD, Shields CR (1984) Tripartite structure of the Brassica campestris mitochondrial genome. Nature 307:437–440
Pring DR, Lonsdale DM (1985) Molecular biology of higher plant mitochondrial DNA. Int Rev Cytol 97:1–46
Pruitt KD, Hanson MR (1991) Transcription of the Petunia mitochondrial cms-associated pcf locus in male-sterile and fertility-restored lines. Mol Gen Genet 227:348–355
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
Saumitou-Laprade P, Pannenbecker G, Maggouta F, Jean R, Michaelis G (1989) A linear 10.4-kb plasmid in the mitochondria of Beta maritima. Curr Genet 16:181–186
Schuster W, Brennicke A (1986) Pseudocopies of the ATPasesubunit gene in Oenothera mitochondria are present on different circular molecules. Mol Gen Genet 204:29–35
Van Damme JMM (1983) Gynodiocey in Plantago lanceolata L. II. Inheritance of three male sterility types. Heredity 50:253–273
Weihe A, Dudareva NA, Veprev SG, Maletsky SI, Melzer R, Salganik RI, Borner Th (1991) Molecular characterization of mitochondrial DNA of different subtypes of male-sterile cytoplasms of the sugar beet Beta vulgaris. L. Theor Appl Genet 82:11–16
Author information
Authors and Affiliations
Additional information
Communicated by G. Wenzel
Rights and permissions
About this article
Cite this article
Saumitou-Laprade, P., Rouwendal, G.J.A., Cuguen, J. et al. Different CMS sources found in Beta vulgaris ssp maritima: mitochondrial variability in wild populations revealed by a rapid screening procedure. Theoret. Appl. Genetics 85, 529–535 (1993). https://doi.org/10.1007/BF00220909
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00220909