Skip to main content
SpringerLink
Log in

Characterization of binding and receptors for epidermal growth factor in smooth muscle

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

The mitogenic and differentiation-inducing activities of epidermal growth factor (EGF) in epithelial tissues have been well described. Since non-mitogenic effects of EGF, especially in mesenchymal tissues such as smooth muscle are not well-known (Nanney et al. 1984), we have examined EGF-binding and receptors in smooth muscle from many sites. Specific EGF binding sites were detected by incubating small pieces of tissue with 125I-EGF; immunoreactive EGF receptors were detected by immunohistochemistry. In-situ localization of 125I-EGF binding sites and immunoreactive EGF receptors of smooth muscle cells in intact mammalian tissues were identical using either 125I-EGF autoradiography or anti-EGF receptor antibody in an immunoperoxidase method. Cultured rat aortic smooth muscle also contained specific EGF receptors as detected by their biological response to EGF-binding and internalization of 125I-EGF, as well as EGF-stimulated phosphorylation of a 170K protein. The presence of EGF receptors in a well-differentiated smooth muscle cell indicates that EGF may play a physiological, but non-mitogenic role in mammalian tissues in vivo.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bahn RS, Speeg KV, Ascoli M, Rabin D (1980) Epidermal growth factor stimulates production of progesterone in cultured human choriocarcinoma cells. Endocrinology 107:2121–2123

    Google Scholar 

  • Barnes D, Colowick SP (1976) Stimulation of sugar uptake in cultured fibroblasts by epidermal growth factor (EGF) and EGF-binding arginine esterase. J Cell Physiol 89:633–640

    Google Scholar 

  • Beneviste R, Speeg KV, Carpenter G, Cohen S, Linder J, Rabinowitz D (1978) Epidermal growth factor stimulates secretion of human chorionic gonadotropin by cultured human choriocarcinoma cells. J Clin Endocrinol Metab 46:169–172

    Google Scholar 

  • Berk BC, Brock TA, Webb RC, Taubman MB, Atkinson WJ, Gimbrone MA (1985) Epidermal growth factor, a vascular smooth muscle mitogen, induces rat aortic contraction. J Clin Invest 75:1083–1086

    Google Scholar 

  • Bhargava G, Rifas L, Makman MH (1979) Presence of epidermal growth factor receptors and influence of epidermal growth factor on proliferation and aging in cultured smooth muscle cells. J Cell Physiol 100:365–374

    Google Scholar 

  • Björkerud S (1979) Mechanisms of atherosclerosis. Pathobiol Annu 9:277–289

    Google Scholar 

  • Björkerud S (1985) Cultivated human arterial smooth muscle displays heterogeneous pattern of growth and phenotypic variation. Lab Invest 53:303–310

    Google Scholar 

  • Buhrow SA, Cohen S, Staros JV (1982) Affinity labeling of the protein kinase associated with the epidermal growth factor receptor in membrane vesicles from A-431 cells. J Biol Chem 257:4019–4022

    Google Scholar 

  • Carpenter G (1981) Epidermal growth factor. Handbook of Exp Pharmacol 57:90–126

    Google Scholar 

  • Carpenter G, Cohen S (1976) 125I-Labelled human epidermal growth factor. Binding, internalization and degradation in human fibroblasts. J Cell Biol 71:159–171

    Google Scholar 

  • Carpenter G, King LE, Cohen S (1979) Rapid enhancement of protein phosphorylation in A-431 cell membrane preparations by epidermal growth factor. J Biol Chem 254:4884–4889

    Google Scholar 

  • Chamley-Campbell JH, Campbell GR, Ross R (1981) Phenotypedependent response of cultured aortic smooth muscle to serum mitogens. J Cell Biol 89:379–383

    Google Scholar 

  • Chinkers M, McKanna JA, Cohen S (1981) Rapid rounding of human epidermal carcinoma cells A-431 induced by epidermal growth factor. J Cell Biol 88:422–429

    Google Scholar 

  • Clemmons DR (1984) Interactions of circulating cell-derived and plasma growth factors in stimulating cultured smooth muscle cell replication. J Cell Physiol 121:425–430

    Google Scholar 

  • Cohen S, Ushiro H, Stoscheck CM, Chinkers M (1982) A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles. J Biol Chem 257:1523–1531

    Google Scholar 

  • Comens PG, Simmer RL, Baker JB (1982) Direct linkage of 125I-EGF to cell surface receptors: a useful artifact of chloramine-T treatment. J Biol Chem 257:42–45

    Google Scholar 

  • Diamond I, Legg A, Schneider JA, Rosengurt E (1978) Glycolysis in quiescent cultures of 3T3 cells. J Biol Chem 253:866–877

    Google Scholar 

  • Dunn WA, Connolly TP, Hubbard AL (1986) Receptor-mediated endocytosis of epidermal growth factor by rat hepatocytes: receptor pathway. J Cell Biol 102:24–36

    Google Scholar 

  • Fabricant RN, DeLarco JE, Todaro GJ (1977) Nerve growth factor receptors on human melanoma cells in culture. Proc Natl Acad Sci USA 74:565–569

    Google Scholar 

  • Fernandez-Pol JA (1981) Rapid and specific enhancement of phosphorylation of two normal rat kidney cell membrane polypeptides of Mr=170,000 and 150,000 by epidermal growth factor in vitro. J Biol Chem 256:9742–9749

    Google Scholar 

  • Gallis B, Edelman AM, Casnellie JE, Krebs EG (1983) Epidermal growth factor stimulates tyrosine phosphorylation of the myosin regulatory light chain from smooth muscle. J Biol Chem 258:13089–13093

    Google Scholar 

  • Gospodarowicz D, Hirabayashi K, Giguere L, Tauber J-P (1981) Factors controlling the proliferative rate, final cell density, and life span of bovine vascular smooth muscle cells in culture. J Cell Biol 89:568–578

    Google Scholar 

  • Huot RI, Foidart JM, Nardone RM, Stromberg K (1981) Differential modulation of human chorionic gonadotropin secretion by epidermal growth factor in normal and malignant placental cultures. J Clin Endocrinol Metab 53:1059–1063

    Google Scholar 

  • King LE, Carpenter GF (1983) Epidermal growth factor. In: Goldsmith L (ed) Biochemistry and physiology of the skin. Oxford University Press, New York

    Google Scholar 

  • King LE, Carpenter G, Cohen S (1980) Characterization by electrophoresis of EGF stimulated phosphorylation using A-431 cells. Biochemistry 19:1524–1528

    Article  Google Scholar 

  • Kruth HS (1985) Platelet-mediated cholesterol accumulation in cultured aortic smooth muscle cells. Science 227:1243–1244

    Google Scholar 

  • Lai WH, Guyda HJ (1984) Characterization and regulation of epidermal growth factor receptors in human placental cultures. J Clin Endocrinol Metab 58:344–352

    Google Scholar 

  • Magid M, Nanney LB, Stoscheck CM, King LE (1986) Epidermal growth factor binding and receptor distribution in term human placenta. Placenta 6:519–526

    Google Scholar 

  • Muramatsu I, Hollenberg MD, Lederis K (1985) Vascular actions of epidermal growth factor-urogastrone: possible relationship to prostaglandin production. Can J Physiol Pharmacol 63:994–999

    Google Scholar 

  • Nanney LB, Magid M, Stoscheck CM, King LE (1984a) Comparison of epidermal growth factor binding and receptor distribution in normal human epidermis and epidermal appendages. J Invest Dermatol 83:385–393

    Google Scholar 

  • Nanney LB, McKanna JA, Stoscheck CM, Carpenter G, King LE (1984b) Visualization of epidermal growth factor receptors in human epidermis. J Invest Dermatol 82:165–169

    Google Scholar 

  • Nanney LB, Stoscheck CM, King LE (1984c) Are epidermal growth factor (EGF) receptors markers for smooth muscle cells? J Cell Biol 99:414a

    Google Scholar 

  • Nanney LB, Stoscheck CM, Magid M, King LE (1986) Altered 125I-epidermal growth factor binding and receptor distribution in psoriasis. J Invest Dermatol 86:260–265

    Google Scholar 

  • Oka Y, Orth DN (1983) Human epidermal growth factor-β-urogastrone is associated with blood platelets. J Clin Invest 72:249–259

    Google Scholar 

  • Owen NE (1984) Regulation of Na+/K + /Cl- cotransport in vascular smooth muscle cells. Biochem Biophys Res Commun 125:500–508

    Google Scholar 

  • Owen N (1985) Effect of TPA on ion fluxes and DNA synthesis in vascular smooth muscle cells. J Cell Biol 101:454–459

    Google Scholar 

  • Ross R, Kariya B (1980) Morphogenesis of vascular smooth muscle in atherosclerosis and cell culture. In: Bohr DF, Somlyo AP, Sparks HY (eds) Handbook of Physiology, The Cardiovascular System, Vascular Smooth Muscle, Vol. 2. American Physiology Society, Bethesda, MD

    Google Scholar 

  • Sefton BM, Hunter T, Beemon K, Eckhart W (1980) Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus. Cell 20:807–816

    Article  Google Scholar 

  • Starkey RH, Orth DN (1977) Radioimmunoassay of human epidermal growth factor (urogastrone). J Clin Endocrinol Metab 45:1144–1153

    Google Scholar 

  • Stoscheck CM, Carpenter G (1983a) Characteristics and uses of EGF receptor kinase. Cell Biol Int Rep 7:529–530

    Google Scholar 

  • Stoscheck CM, Carpenter G (1983b) Characteristics of antibodies to the epidermal growth factor receptor-kinase. Arch Biochem Biophys 227:457–468

    Google Scholar 

  • Stoscheck CM, King LE (1986a) The role of epidermal growth factor in carcinogenesis. Cancer Res 46:1030–1037

    Google Scholar 

  • Stoscheck CM, King LE (1986b) Functional and structural characteristics of EGF and its receptor and their relationship to transforming proteins. J Cell Biochem (in press)

  • Wrann MM, Fox CF (1979) Identification of epidermal growth factor receptors in a hyperproducing epidermal carcinoma cell line. J Biol Chem 254:8083–8086

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departments of Plastic Surgery and Cell Biology, Department of Medicine (Dermatology), Vanderbilt University, and Veterans Administration Medical Centers, Nashville, Tennessee, USA

    Lillian B. Nanney Ph.D., Christa M. Stoscheck & Lloyd E. King

Authors
  1. Lillian B. Nanney Ph.D.
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Christa M. Stoscheck
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Lloyd E. King
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nanney, L.B., Stoscheck, C.M. & King, L.E. Characterization of binding and receptors for epidermal growth factor in smooth muscle. Cell Tissue Res. 254, 125–132 (1988). https://doi.org/10.1007/BF00220025

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00220025

Key words

Search

Navigation