Summary
The differentiation of the spermatid, especially in reference to the formation of the flagellum, and transformation of the shape of the nucleus was investigated in the domestic fowl.
In the early stage of the spermatid, a prominent Golgi apparatus appears around the centrioles. The Golgi vesicles then surround the axial-filament complex which develops from the distal centriole. These vesicles fuse to form continuous membrane at the earliest stage of flagellar formation, and in the succeeding stage Golgi lamellae are attached to the plasma membrane of the developing flagellum. From these observations, it is assumed that Golgi apparatus may be a source of the membrane system of the flagellum.
The microtubules distributed around the nucleus form the circular manchette. The anterior region of the nucleus with the manchette is cylindrical in shape and the posterior region without it remains irregular in shape. When the circular manchette has been completed, the whole nucleus acquires a slender cylindrical shape. The circular manchette then changes into the longitudinal manchette. The nuclei of spermatids without a longitudinal manchette are abnormal in shape. In view of these observations it is assumed that the nuclear shaping of the spermatid may be accomplished by circular manchette and the maintenance of shape of the elongated nucleus by longitudinal manchette.
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Amsterdam, A., Ohad, I., Schramm, M.: Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle. J. Cell Biol. 41, 753–773 (1969)
Burgos, M.H., Fawcett, D.W.: Studies on the fine structure of the mammalian testis. I. Differentiation of the spermatids in the cat (Felis domestica). J. biophs. biochem. Cytol. 1, 287–300 (1955)
Fawcett, D.W.: The anatomy of the mammalian spermatozoon with particular reference to the guinea pig. Z. Zellforsch. 67, 279–296 (1965)
Fawcett, D.W., Anderson, W.A., Phillips, D.M.: Morphogenetic factors influencing the shape of the sperm head. Develop. Biol. 26, 220–251 (1971)
Grigg, G.W., Hodge, A.J.: Electron microscopic studies of spermatozoa. I. The morphology of the spermatozoon of the common domestic fowl (Gallus domesticus). Aust. J. Sci. Res. B 2, 271–286 (1949)
Karnovsky, M.J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27, 137A-138A (1965)
Kessel, R.G.: Spermiogenesis in the dragonfly with special reference to a consideration of the mechanisms involved in the development of cellular asymmetry. In: Comparative spermatology, pp. 531–552 (B. Baccetti, ed.). New York-London: Academic Press 1970
Lake, P.E., Smith, W., Young, D.: The ultrastructure of the ejaculated fowl spermatozoon. Quart. J. exp. Physiol. 53, 356–366 (1968)
Leblond, C.P., Clermont, Y.: Spermiogenesis of rat, mouse, hamster and guinea pig as revealed by the “periodic acid-fuchsin sulfurous acid” technique. Amer. J. Anat. 90, 167–215 (1952)
McIntosh, J.R., Porter, K.R.: Microtubules in the spermatids of the domestic fowl. J. Cell Biol. 35, 153–173(1967)
Nagano, T.: Fine structure of the sperm tail of the domestic fowl (Gallus domesticus). J. appl. Phys. 31, 1844 (1960)
Nagano, T.: Observations on the fine structure of the developing spermatid in the domestic chicken. J. Cell Biol. 14, 193–205 (1962)
Nicander, L., Hellström, B.: Increased thickness of the inner mitochondrial membrane during sperm maturation in the domestic rooster. Exp. Cell Res. 48, 622–625 (1967)
Pehlemann, F.-W.: Die amitotische Zellteilung. Eine elektronenmikroskopische Untersuchung an Interrenalzellen von Rana temporaria L. Z. Zellforsch. 84, 516–548 (1968)
Phillips, D.M.: Development of spermatozoa in the wooly opossum with special reference to the shaping of the sperm head. J. Ultrastruct. Res. 33, 369–380 (1970)
Phillips, D.M.: Spermiogenesis. New York-London: Academic Press 1974a
Phillips, D.M.: Nuclear shaping in the absence of microtubules in scorpion spermatids. J. Cell Biol. 62, 911–917 (1974b)
Rattner, J.B.: Nuclear shaping in marsupial spermatids. J. Ultrastruct. Res. 40, 498–512 (1972)
Rattner, J.B., Brinkley, B.R.: Ultrastructure of mammalian spermiogenesis. I. A tubular complex in developing sperm of the cottontop marmoset Sequinus oedipus. J. Ultrastruct. Res. 32, 316–322 (1970)
Rattner, J.B., Brinkley, B.R.: Ultrastructure of mammalian spermiogenesis. III. The organization and morphogenesis of the manchette during rodent spermiogenesis. J. Ultrastruct. Res. 41, 209–218 (1972)
Rattner, J.B., Olson, G.: Observations on the fine structure of the nuclear ring of the mammalian spermatid. J. Ultrastruct. Res. 43, 438–444 (1973)
Tingari, M.D.: Observations on the fine structure of spermatozoa in the testis and excurrent ducts of the male fowl, Gallus domesticus. J. Reprod. Fertil. 34, 255–265 (1973)
Uchida, T.A., Mori, T.: Electron microscope studies on the fine structure of germ cells in Chiroptera. I. Spermiogenesis in some bats and notes on its phylogenetic significance. Sci. Bull. Fac. Agr., Kyushu Univ. 26, 399–418 (1972)
Whaley, W.G., Mollenhauer, H.H.: The Golgi apparatus and cell plate formation — A postulate. J. Cell Biol. 17, 216–221 (1963)
Zlotnik, I.: A nuclear ring in the developing male germ cells of dog and cat. Nature (Lond.) 151, 670 (1943)
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The authors wish to thank Mr. Takayuki Mori for his helpful suggestions and technical advices
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Okamura, F., Nishiyama, H. The early development of the tail and the transformation of the shape of the nucleus of the spermatid of the domestic fowl, Gallus gallus . Cell Tissue Res. 169, 345–359 (1976). https://doi.org/10.1007/BF00219607
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DOI: https://doi.org/10.1007/BF00219607