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Effect of prolactin on DNA methylation in the liver and kidney of rat

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Abstract

Prolactin is an important growth modulatory hormone in fetal and adult tissues. It stimulates DNA synthesis and enzymatic markers of the G1 phase of cell cycle in rat liver and other tissues. In this study the effects of prolactin on 5-methyl cytosine content in liver and kidney of rats was studied using HPLC. Prolactin treatment caused hypomethylation of DNA in the liver and kidney of immature rats at 48 h after treatment and the effect remained even at 72 h. Prolactin also caused hypomethylation of DNA in the kidney and liver of adult rats at 48 h after treatment. These results indicate that prolactin probably regulates DNA methylation in the liver and kidney of immature and adult rats.

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References

  1. Riggs AD, Jones PA: 5-Methylcytosine, gene regulation and cancer. Adv Cancer Res 40: 1–30, 1983

    Google Scholar 

  2. Razin A, Riggs AD: DNA methylation and gene function. Science 210: 604–610, 1980

    Google Scholar 

  3. Doerfler W: DNA methylation and gene activity. Ann Rev Biochem 52: 93–124, 1983

    Google Scholar 

  4. Holliday R, Pugh JE: DNA modification mechanism and gene activity during development. Science 187: 226–232, 1975

    Google Scholar 

  5. Razin A, Syzf M: DNA methylation patterns. Biochim Biophys Acta 782: 331–342, 1984

    Google Scholar 

  6. Ehrlich M, Wang RYH: 5-Methylcytosine in eukaryotic DNA. Science 212: 1350–1357, 1981

    Google Scholar 

  7. Jaenisch R, Jahner D: Methylation, expression and chromosomal position of genes in mammals. Biochim Biophys Acta 782: 1–9, 1984

    Google Scholar 

  8. Wilks AF, Cozens PJ, Mattaj IW, Jost JP: Estrogen induces a demethylation at the 5′-end region of the chicken vitellogenin gene. Proc Natl SciAcad USA 79: 4252–4255, 1982

    Google Scholar 

  9. Romanov GA, Vanyushin BF: Methylation of reiterated sequences in mammalian DNAs. Effect of the tissue type age, malignancy and hormonal induction. Biochim Biophys Acta 653: 204–218, 1981

    Google Scholar 

  10. White R, Parker M: Developmental changes in DNA methylation around prostatic steroid-binding protein genes. J Biol Chem 258: 8943–8948, 1983

    Google Scholar 

  11. Lu L-JW, Liehr JG, Sirbasku DA, Randerath E, Randerath K: Hypomethylation of DNA in estrogen-induced and -dependent hamster kidney tumors. Carcinogenesis 9: 925–929,1988

    Google Scholar 

  12. Nicoll CS: in: Progress in prolactin physiology and pathology (Robin C. Harter M. eds.), Elsevier/North Holland Biomedical Press, Amsterdam-New York, 1978, pp 175–188

  13. Russell DH, Larson DF, Cardon SB, Copeland JG: Cyclosporine inhibits prolactin induction of ODC in rat tissues. Molec Cell Endocrinol 35: 159–166, 1984

    Google Scholar 

  14. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning, A Laboratory manual, Cold Spring Harbor Laboratory Press, New York, 1982

    Google Scholar 

  15. Buckley AR, Putnam CW, Montgomery DW, Russell DH: Prolactin administration stimulates rat hepatic DNA synthesis. Biochem Biophys Res Commun 138: 1138–1145, 1986

    Google Scholar 

  16. Rosa AAM, Djiane J, Houdebine LM, Kelly PA: Stimulatory effects of prolactin and anti-prolactin receptor serum on prolactin binding sites in rat liver cells in suspension culture. Biochem Biophys Res Commun 106, 243–249,1982

    Google Scholar 

  17. Roy AK, Chatterjee B, Desphande AK: Hormone dependent expression of α 2 u globulin gene in rat liver. In: Gene regulation by steroid hormones (Roy AK and Clark JH Eds.) Springer-Verlag, 1980, pp 230–246.

  18. Mourelle M, Rubalcava B: Regeneration of the liver after carbon tetrachloride: Differences in adenylate cyclase and pancreatic hormone receptors. J Biol Chem 256: 1656–1660,1981

    Google Scholar 

  19. Buckley AR, Putnam CW, Russell DH: Prolactin is a tumor promoter in rat liver. Life Sciences 37: 2569–2575,1985

    Google Scholar 

  20. Gout PW, Beer CT, Noble RL: Prolactin-stimulated growth of cell cultures established from malignant Nb rat lymphomas. Cancer Res 40: 2433–2436, 1980

    Google Scholar 

  21. Noble RL, Beer CT, Gout PW: Evidence in vivo and in vitro of a role for the pituitary in the growth of malignant lymphomas in Nb rats. Cancer Res 40: 2437–2440, 1980

    Google Scholar 

  22. O'Brien TG: The effect of colchine on the induction of ODC by 12-O-tetradecoyl-phorbol-13 acetate. Cancer Res. 36:2644–2653,1976

    Google Scholar 

  23. Buckley AR, Putnam CW, Russell DH: Rapid elevation of plasminogen activator activity in rat tissues by prolactin. Biochem Biophys Res Commun 122: 1005–1011, 1984

    Google Scholar 

  24. Jones PA: DNA methylation and cancer. Cancer Res 46: 461–466,1986

    Google Scholar 

  25. Feinberg AP, Vogelstein B: Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature 301: 89–92, 1983

    Google Scholar 

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Authors and Affiliations

  1. School of Life Sciences, University of Hyderabad, 500 134, Hyderabad, India

    P. M. S. Reddy & P. R. K. Reddy

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  1. P. M. S. Reddy
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  2. P. R. K. Reddy
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Reddy, P.M.S., Reddy, P.R.K. Effect of prolactin on DNA methylation in the liver and kidney of rat. Mol Cell Biochem 95, 43–47 (1990). https://doi.org/10.1007/BF00219528

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  • DOI: https://doi.org/10.1007/BF00219528

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