Skip to main content
SpringerLink
Log in

Compartments in the organum vasculosum laminae terminalis of the rat and their delineation against the outer cerebrospinal fluid-containing space

  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Summary

Using intravenously injected horseradish peroxidase (HRP) as tracer, we demonstrate, that — in contrast to other neurohemal regions — the organum vasculosum laminae terminalis (OVLT) is composed of two functionally different divisions. Both parts of the OVLT are endowed with fenestrated capillaries which, however, obviously differ in their permeability for HRP. In one of these portions the neurohemal region remains unlabeled under the experimental conditions used, while the other portion, in analogy to the majority of neurohemal regions, is labeled by the tracer. The functionally different divisions of the OVLT are separated from one another by tanycytic processes and meningeal cells establishing a barrier between the two hemal compartments. The meningeal elements penetrate the organ in the form of an uninterrupted layer; they are continuous with the pia mater and produce large amounts of basal lamina-like material. Furthermore, they provide the delineation of the OVLT against the outer cerebrospinal fluid-containing compartment, a structural feature that is characteristic of both divisions of the OVLT and corresponds to the arrangement of meninges in all other portions of the brain where a blood vessel penetrates its surface.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bosler O (1977) The organum vasculosum laminae terminalis. A cytological study in the duck, Anas platyrhynchos. Cell Tissue Res 182:382–399

    Google Scholar 

  • Bouchaud C (1975) Données ultrastructurales sur la perméabilité des capillaires des organes circumventriculaires du cerveau. J Microsc Biol Cell 24:45–58

    Google Scholar 

  • Breemen VL van, Clemente CD (1955) Silver deposition in the central nervous system and the hematoencephalic barrier studied with the electron microscope. J Biophys Biochem Cytol 1:161–165

    Google Scholar 

  • Broadwell RD, Brightman MW (1976) Entry of peroxidase into neurons of the central and peripheral nervous systems from extracerebral blood. J Comp Neurol 166:257–284

    Google Scholar 

  • Clementi F, Palade GE (1969) Intestinal capillaries. I. Permeability to peroxidase and ferritin. J Cell Biol 41:33–58

    Google Scholar 

  • Dretzki J (1971) Licht- und elektronenmikroskopische Untersuchungen zum Problem der Blut-Hirn-Schranke circumventriculärer Organe der Ratte nach Behandlung mit Myofer. Z Anat Entwickl Gesch 134:278–297

    Google Scholar 

  • Duvernoy H, Koritké JG (1964) L'angioarchitectonie comparée de l'organe vasculaire de la lame terminale et de l'area postrema chez les Oiseaux. Acta Anat (Basel) 54:354–355

    Google Scholar 

  • Gähwiler BH (1981) Organotypic monolayer cultures of nervous tissue. J Neurosci Methods 4:329–342

    Google Scholar 

  • Hart TK, Pino RM (1985) Variations in capillary permeability from apex and crypt in the villus of the ileo-jejunum. Cell Tissue Res 241:305–315

    Google Scholar 

  • Hart TK, Pino RM (1986) Capillary permeability in the pancreas and colon: restriction of exogenous and endogenous molecules by fenestrated endothelia. Am J Anat 175:49–58

    Google Scholar 

  • Kanwar YS, Farquhar MG (1979) Anionic sites of the glomerular basement membrane. J Cell Biol 81:137–153

    Google Scholar 

  • Krisch B (1986) The functional and structural borders between the CSF- and blood-dominated milieus in the choroid plexuses and the area postrema of the rat. Cell Tissue Res 245:101–115

    Google Scholar 

  • Krisch B, Buchheim W (1984) Access and distribution of exogenous substances in the intercellular clefts of the rat adenohypophysis. Cell Tissue Res 236:439–452

    Google Scholar 

  • Krisch B, Leonhardt H, Buchheim W (1978a) The functional and structural border of the neurohemal region of the median eminence. Cell Tissue Res 192:327–339

    Google Scholar 

  • Krisch B, Leonhardt H, Buchheim W (1978b) The functional and structural border between the CSF- and blood-milieu in the circumventricular organs (organum vasculosum laminae terminalis, subfornical organ, area postrema) of the rat. Cell Tissue Res 195:485–497

    Google Scholar 

  • Krisch B, Leonhardt H, Oksche A (1983) The meningeal compartments of the median eminence and the cortex. A comparative analysis in the rat. Cell Tissue Res 228:597–640

    Google Scholar 

  • Krisch B, Leonhardt H, Oksche A (1984) Compartments and perivascular arrangement of the meninges covering the cerebral cortex of the rat. Cell Tissue Res 238:459–474

    Google Scholar 

  • Leduc EH, Wislocki GB (1952) Vital staining of hemato-encephalic barrier and cytological comparison of the neurohypophysis, pineal body, area postrema, intercolumnar tubercle and supraoptic crest. J Comp Neurol 96:371–398

    Google Scholar 

  • Leonhardt H (1980) Ependym und circumventrikuläre Organe. In: Oksche A, Vollrath L (eds) Handbuch der mikroskopischen Anatomie des Menschen. Neuroglia I. Nervensystem, 10. Teil. Springer, Berlin Heidelberg New York, pp 177–666

    Google Scholar 

  • Leonhardt H, Krisch B, Hartwig H-G (1983) Circumventricular organs as targets and release sites for peptide hormones and monoamines. In: Sano Y, Ibata Y, Zimmerman EA (eds) Structure and function of peptidergic and aminergic neurons. Japan Scientific Societies Press, Tokyo, VNU Science Press BV, Utrecht, The Netherlands, pp 55–71

    Google Scholar 

  • Luft JH (1971) Ruthenium red and violett. II. Fine structural localization in animal tissues. Anat Rec 771:369–416

    Google Scholar 

  • McDermott JR, Smith AI, Biggins JA, Hardy JA, Dodd PR, Edwardson JA (1981) Degradation of luteinizing hormone-releasing hormone by serum and plasma in vitro. Regul Pept 2:69–70

    Google Scholar 

  • Mergner H (1959) Untersuchungen am Organon vasculosum laminae terminalis (Crista supraoptica) im Gehirn einiger Nagetiere. Zool Jb Abt Anat Ontog 77:290–356

    Google Scholar 

  • Patel YG, Wheatley T (1983) In vivo and in vitro plasma disappearance and metabolism of somatostatin-28 and somatostatin-14 in the rat. Endocrinology 112:220–225

    Google Scholar 

  • Pearse AGE (1968) Histochemistry — theoretical and applied 3rd ed Vol 1. Little, Brown and Co., Boston

    Google Scholar 

  • Peters GE (1982) Distribution and metabolism of exogenous somatostatin in the rat. Regul Pept 3:213–226

    Google Scholar 

  • Pino RM (1986a) Restriction of exogenous transthyretin (prealbumin) by the endothelium of the rat choriocapillaris. Am J Anat 177:63–70

    Google Scholar 

  • Pino RM (1986b) The cell surface of a restrictive fenestrated endothelium. II. Dynamics of cationic ferritin binding and the identification of heparin and heparan sulfate domains on the choriocapillaris. Cell Tissue Res 243:157–164

    Google Scholar 

  • Pino RM, Essner E (1980) Structure and permeability to ferritin of the choriocapillary endothelium of the rat eye. Cell Tissue Res 208:21–27

    Google Scholar 

  • Pino RM, Essner E (1981) Permeability of rat choriocapillaris to hemeproteins. Restriction of tracers by a fenestrated endothelium. J Histochem Cytochem 29:281–290

    Google Scholar 

  • Pino RM, Thouron CL (1983) Vascular permeability in the rat eye to endogeneous albumin and immunoglobulin G (IgG) examined by immunohistochemical methods. J Histochem Cytochem 31:411–416

    Google Scholar 

  • Röhlich P, Wenger T (1969) Elektronenmikroskopische Untersuchungen am Organon vasculosum laminae terminalis der Ratte. Z Zellforsch 102:483–506

    Google Scholar 

  • Simionescu M, Simionescu N, Palade GE (1976) Structural-functional correlates in the transendothelial exchange of water-soluble macromolecules. Thromb Res 8:247–256

    Google Scholar 

  • Simionescu N (1983) Cellular aspects of transcapillary exchange. Physiol Rev 63:1536–1579

    Google Scholar 

  • Wartenberg H, Hadziselimovic F, Seguchi H (1973) Experimentelle Untersuchungen über die Passage der Blut-Gewebs-Schranke in den zirkumventrikulären Organen des Meerschweinchengehirns. Anat Anz 130:345–355

    Google Scholar 

  • Weindl A (1974) Structural and functional investigations on the mammalian organum vasculosum of the lamina terminalis. Thesis, University of Rochester

  • Weindl A, Joynt RJ (1972) The median eminence as a circumventricular organ. In: Knigge KM, Scott DE, Weindl A (eds) Brain-endocrine interaction. Median eminence, structure and function. Karger, Basel, pp 190–200

    Google Scholar 

  • Weindl A, Schinko I (1975) Vascular and ventricular neurosecretion in the organum vasculosum of the lamina terminalis of the golden hamster. In: Knigge KM, Scott DE, Kobayashi H, Ishii S (eds) Brain-endocrine interaction II. The ventricular system in neuroendocrine mechanisms. Karger, Basel, pp 190–200

    Google Scholar 

  • Weindl A, Schwink A, Wetzstein R (1967a) Der Feinbau des Gefäßorgans der Lamina terminalis beim Kaninchen. I Die Gefäße. Z Zellforsch 79:1–48

    Google Scholar 

  • Weindl A, Schwink A, Wetzstein R (1967b) Elektronenmikroskopische Untersuchungen am Gefäßorgan der Lamina terminalis des Kaninchens. Anat Anz 120 Erg.-H:189–901

    Google Scholar 

  • Wenger T, Röhlich P (1969) Electron microscopical investigation of the organon vasculosum laminae terminalis in the rat and in the rhesus monkey. In: Sterba G (ed) Circumventricular Organs and Cerebrospinal Fluid. VEB G Fischer, Jena, pp 167–171

    Google Scholar 

  • Wenger T, Törö I (1971) Studies on the organon vasculosum laminae terminalis. IV. Fine structure of the organon vasculosum laminae terminalis in man. Acta Biol Acad Sci Hung 22:(3) 331–342

    Google Scholar 

  • Westergaard E (1975) Enhanced vesicular transport of exogenous peroxidase across cerebral vessels, induced by serotonin. Acta Neuropathol 32:27–42

    Google Scholar 

  • Wislocki GB, King LS (1936) The permeability of the hypophysis and hypothalamus to vital dyes, with a study of the hypophyseal vascular supply. Am J Anat 58:421–472

    Google Scholar 

  • Wislocki GB, Leduc EH (1952) Vital staining of the hematoencephalic barrier by silver nitrate and trypan blue and cytological comparison of the neurohypophysis, pineal body, area postrema, intercolumnar tubercle and supraoptic crest. J Comp Neurol 96:371–413

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Anatomisches Institut der Universität Kiel, Kiel, Bundesrepublik Deutschland

    Brigitte Krisch & Helmut Leonhardt

  2. Institut für Anatomie und Zytobiologie der Universität Giessen, Giessen, Bundesrepublik Deutschland

    Andreas Oksche

Authors
  1. Brigitte Krisch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Helmut Leonhardt
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Andreas Oksche
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by the Deutsche Forschungsgemeinschaft (Grant Nr. Kr 569/5-2) and the Stiftung Volkswagenwerk

Rights and permissions

Reprints and permissions

About this article

Cite this article

Krisch, B., Leonhardt, H. & Oksche, A. Compartments in the organum vasculosum laminae terminalis of the rat and their delineation against the outer cerebrospinal fluid-containing space. Cell Tissue Res. 250, 331–347 (1987). https://doi.org/10.1007/BF00219078

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00219078

Key words

Search

Navigation