Summary
The presence of an S-100-like immunoreactivity was investigated in the planarian Dugesia gonocephala. By microcomplement fixation assay, measurable amounts of S-100-like immunoreactive material (0.11μg/mg soluble protein) were detected in planarian high-speed supernatants. The index of immunological dissimilarity between ox S-100 and planarian S-100-like immunoreactive material was higher than that previously calculated between ox S-100 and all the vertebrates tested. By the immunohistochemical PAP method, S-100-like immunoreactivity was only detectable in the cilia of the epidermal cells. Although the biological meaning of S-100-like immunoreactivity in planarian remains to be clarified, the present data introduce new perspectives into the investigation of S-100.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Ballal NR, Goldberg DA, Busch H (1975) Dissociation and reconstitution of chromatin appreciable degradation of proteins. Biochem Biophys Res Commun 62:972–982
Bock E (1978) Nervous system specific proteins. J Neurochem 30:7–14
Bustin M, Hopkins RB, Isenberg I (1978) Immunological relatedness of high mobility group chromosomal proteins from calf thymus. J Biol Chem 253:1694–1699
Cocchia D (1981) Immunocytochemical localization of S-100 protein in the brain of adult rat. Anultrastructural study. Cell Tissue Res 214:529–540
Cocchia D, Miani N (1980) Immunocytochemical localization of the brain-specific S-100 protein in the pituitary gland of adult rat. J Neurocytol 9:771–782
Cocchia D, Michetti F (1981) S-100 antigen in satellite cells of the adrenal medulla and the superior cervical ganglion of the rat. An immunochemical and immunocytochemical study. Cell Tissue Res 215:103–112
Cocchia D, Michetti F, Donato R (1981) Immunochemical and immunocytochemical localization of S-100 antigen in normal human skin. Nature (Lond) 294:85–87
Davis BJ (1964) Disc-electrophoresis. II. Method and application to human serum proteins. Ann NY Acad Sci 121:404–427
Gaynor R, Irie R, Morton D, Herschman HR (1980) S-100 protein is present in cultured human malignant melanomas. Nature (Lond) 286:400–401
Gremigni V, Domenici L (1974) Electron microscopical and cytochemical study of vitelline cells in the fresh water triclad Dugesia lugubris s. l. I. Origin and morphogenesis of cocoon-shell globules. Cell Tissue Res 150:261–270
Harboe N, Ingild A (1973) Immunization, isolation of immunoglobulins, estimation of antibody titre. Scand J Immunol 2 (Suppl 1):161–164
Kessler D, Levine L, Fasman G (1968) Some conformational and immunological properties of a bovine brain acidic protein (S-100). Biochemistry 7:758–764
Larrabee MG, Klingman JD, Leicht WS (1963) Effects of temperature, calcium and activity on phospholipid metabolism in a sympathetic ganglion. J Neurochem 10:549–570
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biochem 193:265–275
Møller M, Ingild A, Bock E (1978) Immunohistochemical demonstration of S-100 protein and GFA protein in interstitial cells of rat pineal gland. Brain Res 140:1–13
Moore BW (1965) A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun 19:739–744
Moore BW (1969) Acidic proteins. In: Lajtha A (ed) Chemical architecture of the nervous system. Plenum Press, New York, pp 93–99
Moore BW, Perez VJ (1966) Complement fixation for antigens at a picogram level. J Immunol 96:1000–1005
Nakajima T, Yamaguchi H, Takahashi K (1980) S-100 protein in folliculostellate cells of the rat pituitary lobe. Brain Res 191:523–531
Nonno L, Herschman H, Levine L (1969) Serologic comparisons of the carbonic anhydrases of primateerythrocytes. Arch Biochem Biophys 136:361–367
Ornstein L (1964) Disc-electrophoresis. I. Background and theory. Ann NY Acad Sci 121:321–349
Sarich VM, Wilson AC (1966) Quantitative immunochemistry and the evolution of primate albumins: Microcomplement fixation. Science 154:1563–1566
Sternberger LA, Hardy PH Jr, Cuculis JJ, Meyer HG (1970) The unlabelled antibody enzyme method of immunohistochemistry. Preparation and properties of soluble antigen-antibody complex (horse-radish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. JHistochem Cytochem 18:315–333
Wilson AC, Kaplan NO, Levine L, Pesce A, Reichlin M, Allison WS (1964) Evolution of lacticdehydrogenases. Federation Proc 23:1258–1266
Zomzely-Neurath CE, Walker WA (1980) Nervous system-specific proteins: 14–3–2 protein, neuron-specific enolase, and S-100 protein. In: Bradshaw RA, Schneider DM (eds) Proteins of the nervous system. Raven Press, New York, pp 1–57
Zuckerman JE, Herschman HR, Levine L (1970) Appearance of a brain-specific antigen (the S-100 protein) during human foetal development. J Neurochem 17:247–251
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Michetti, F., Cocchia, D. S-100-like immunoreactivity in a planarian. Cell Tissue Res. 223, 575–582 (1982). https://doi.org/10.1007/BF00218477
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218477